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121.
Chitin, chitosan and Dowex A 1 chelating polymers powders, 100 to 200 mesh, were used in column chromatography to remove methyl mercury acetate from various waters, including sea water and a dilute acetic acid - acetaldehyde solution. Methyl mercury acetate was labeled with203Hg and chromatography was followed by γ-ray spectrometry. Results show that chitosan and Dowex A 1 are particularly effective in collecting Hg, even though the metalorganic compounds are not fixed so strongly as the simple ions. Elution can be performed with hot water. A good volume reduction factor can be obtained and the results might be applied in Hg pollution prevention. 相似文献
122.
Rinaldo Botondi Matteo Vailati Andrea Bellincontro Riccardo Massantini Roberto Forniti Fabio Mencarelli 《Postharvest Biology and Technology》2009,51(1):97-103
Little is known about the biochemistry of the marron (Castanea sativa cv Marrone fiorentino) when it is soaked in water for the curing process; the immersion treatment time is still empirical and based on traditional knowledge. A study was carried out by keeping marrons in water for 7 d at 14 °C. Tap water and acid water (pH 3) were used in the following water/marron ratios 1:1, 1:2, and 3:2. After curing, the marrons were moved to air storage at room temperature (20 °C and 90% RH) for 1 week (shelf-life), and at 0 °C and 90% RH for 60 d. A large accumulation of carbon dioxide in the head space of the glass jar where the marrons were kept, was observed during water soaking, above all in samples in acid water with a water/marron ratio 1:2. In the first 5 d of water curing, a significant increase in acetaldehyde concentration and phenol content was measured in the marron pulp and subsequently, both compounds declined. Ethanol decreased slightly or remained constant. During the following week of shelf-life, the lowest respiration rate was measured in 1:1 and 1:2 samples while the highest rate was measured in acid water samples. SSC (solid soluble contents) increased in marrons soaked in acid water but remained constant under shelf-life conditions. SSC, ethanol, acetaldehyde and polyphenols did not change following storage at 0 °C for 2 months, but acid water samples showed a higher percentage of decayed marrons. Finally, it is possible to define some technological parameters. The immersion time of marrons in water must be at least 5 and no longer than 7 d, in order to avoid cell destruction. A water/marron ratio of 1:1 is advised. Using acid water, the samples with the ratios of 1:2 and 1:1 showed higher polyphenol and sugar contents. However, the metabolic response of these marrons was assumed to be a “stress response” to acid water. 相似文献
123.
Sea bream (Sparus aurata) production plays a significant part in Italian aquaculture, contributing to almost 18% of national pisciculture sales revenue. In recent years, Italian firms faced higher competition from countries with lower production costs. This prompted responses toward both cost reduction and product differentiation. The objective of this study was to investigate the preferences of Italian consumers for sea bream from fish farms, with a focus on aspects of product differentiation as gleaned from the analysis of the market situation: price, product origin, type and place of fish farming, and, in particular, type of feed. Data were collected with a consumers’ survey using personal interviews conducted on a questionnaire that included a choice experiment. Consumer preferences were analyzed with choice models based on stated preference data. The models made it possible to evaluate the potential of products with different combinations of attributes for which there is currently no market information available. In particular, the country of origin emerged as an important element of consumer choice, and to a lesser degree, organic certification and fish farming in marine cages also play a relevant role and may command a price premium. 相似文献
124.
Amorati R Pedulli GF Cabrini L Zambonin L Landi L 《Journal of agricultural and food chemistry》2006,54(8):2932-2937
The antioxidant activity of several phenolic acids and esters has been investigated both in organic solutions and in large unilamellar phosphatidylcholine vesicles. In solution these compounds behaved as good antioxidants, with the exception of protocatechuic acid, due to the presence of the catechol moiety. Because their antioxidant activity followed an inverse dependence on the magnitude of their O-H bond dissociation enthalpies (BDE), the key mechanism of the chain-breaking action was attributed to hydrogen atom transfer (HAT) from the phenolic OH to peroxyl radicals. In unilamellar vesicles the antioxidant activity was strongly dependent on the pH of the buffer solution. In acid media (pH 4) all of the examined phenolic acids or esters behaved as weak inhibitors of peroxidation, whereas, with increasing pH, their antioxidant activity increased substantially, becoming comparable to or even better than that of Trolox. At pH 8 they also gave rise to lag phases 2-3 times longer than that of Trolox. The increased activity being observed in proximity of the pK(a) value corresponding to the ionization of one of the catecholic hydroxyl groups, this effect has been attributed to the high antioxidant activity of the phenolate anion. 相似文献
125.
Aspects of phosphorus transfer from soils in Europe 总被引:1,自引:0,他引:1
Imbalanced nutrient management has caused soil phosphorus (P) to become an environmental rather an agronomic problem in more economically developed countries. This subject has been the topic of numerous journal special issues, conferences, and reviews but we consider yet another review of this subject is necessary with the main target of providing a point of view on nonpoint transfer from soils and control strategies for an improved environmental management of P. This review considers the causes of the excessive P transfer from soil to surface water in Europe and the scientific knowledge necessary to develop control strategies. 相似文献
126.
Characterization and role of polyphenol oxidase and peroxidase in browning of fresh-cut melon 总被引:2,自引:0,他引:2
Polyphenol oxidase (PPO) and peroxidase (POD) were extracted from two different varieties of melon ( Cucumis melo L. cantalupensis cv. Charentais and C. melo L. inodorus cv. Amarillo) and characterized using reliable spectrophotometric methods. In both cases the enzymes followed Michaelis-Menten kinetics, showing different values of kinetics parameters between the two cultivars: K m = 7.18 +/- 0.70 mM ('Charentais') and 6.66 +/- 0.20 mM ('Amarillo') mM; V max = 7.93 +/- 0.35 units/min ('Charentais') and 13.82 +/- 0.37 units/min ('Amarillo'), relative to PPO; K m = 24.0 +/- 2.10 mM ('Charentais') and 5.05 +/- 0.19 mM ('Amarillo') mM; V max = 344.83 +/- 10.32 units/min ('Charentais') and 80.64 +/- 2.01 units/min ('Amarillo'), relative to POD. Optimum pH for PPO was 7.0 for 'Charentais' and 7.5 for 'Amarillo, whereas it was 4.5 for both cultivars relative to POD. Melon PPO had maximum activity at 60 degrees C in both 'Charentais' and 'Amarillo' cultivars, whereas POD maximum activity was found at 45 degrees C in 'Charentais' and at 25 degrees C in 'Amarillo'. POD from both cultivars showed higher thermolability compared with PPO, losing >90% of relative activity after only 5 min of incubation at 70 degrees C. POD's activation energy was much higher than that of PPO (Delta E (#) = 86.3 and 160.6 kJ mol (-1) for 'Charentais' and 'Amarillo', respectively). PPO and POD activities from both cultivars showed a decreasing pattern as sugar concentration in the assay medium increased, except in POD extract from 'Charentais', which maintained its activity in the presence of high d-glucose concentration (up to 5 M). Changes in L*, a*, b*, chroma, and hue angle values were chosen to describe the browning development in the samples during storage at 5 degrees C. A slight decrease in L* value and a more marked reduction of a* value were noted in both cultivars above all at the end of storage period. POD activity during storage at 5 degrees C was highly correlated with changes of parameters a*, b*, chroma, and hue angle ( r (2) from 0.82 to 0.97) for cultivar 'Charentais'. According to these results, only POD activity seemed to be involved in browning of minimally processed melon. 相似文献
127.
Daily variations in oligosaccharides of human milk determined by microfluidic chips and mass spectrometry 总被引:2,自引:0,他引:2
Niñonuevo MR Perkins PD Francis J Lamotte LM LoCascio RG Freeman SL Mills DA German JB Grimm R Lebrilla CB 《Journal of agricultural and food chemistry》2008,56(2):618-626
Human milk is a complex biological fluid that provides not only primary nourishment for infants but also protection against pathogens and influences their metabolic, immunologic, and even cognitive development. The presence of oligosaccharides in remarkable abundance in human milk has been associated to provide diverse biological functions including directing the development of an infant's intestinal microflora and immune system. Recent advances in analytical tools offer invaluable insights in understanding the specific functions and health benefits these biomolecules impart to infants. Oligosaccharides in human milk samples obtained from five different individual donors over the course of a 3 month lactation period were isolated and analyzed using HPLC-Chip/TOF-MS technology. The levels and compositions of oligosaccharides in human milk were investigated from five individual donors. Comparison of HPLC-Chip/TOF-MS oligosaccharides profiles revealed heterogeneity among multiple individuals with no significant variations at different stages of lactation within individual donors. 相似文献
128.
D Singh JM Chan P Zoppoli F Niola R Sullivan A Castano EM Liu J Reichel P Porrati S Pellegatta K Qiu Z Gao M Ceccarelli R Riccardi DJ Brat A Guha K Aldape JG Golfinos D Zagzag T Mikkelsen G Finocchiaro A Lasorella R Rabadan A Iavarone 《Science (New York, N.Y.)》2012,337(6099):1231-1235
The brain tumor glioblastoma multiforme (GBM) is among the most lethal forms of human cancer. Here, we report that a small subset of GBMs (3.1%; 3 of 97 tumors examined) harbors oncogenic chromosomal translocations that fuse in-frame the tyrosine kinase coding domains of fibroblast growth factor receptor (FGFR) genes (FGFR1 or FGFR3) to the transforming acidic coiled-coil (TACC) coding domains of TACC1 or TACC3, respectively. The FGFR-TACC fusion protein displays oncogenic activity when introduced into astrocytes or stereotactically transduced in the mouse brain. The fusion protein, which localizes to mitotic spindle poles, has constitutive kinase activity and induces mitotic and chromosomal segregation defects and triggers aneuploidy. Inhibition of FGFR kinase corrects the aneuploidy, and oral administration of an FGFR inhibitor prolongs survival of mice harboring intracranial FGFR3-TACC3-initiated glioma. FGFR-TACC fusions could potentially identify a subset of GBM patients who would benefit from targeted FGFR kinase inhibition. 相似文献
129.
R Desotgiu M Pollastrini C Cascio G Gerosa R Marzuoli F Bussotti 《Tree physiology》2012,32(8):976-986
An experiment in open-top chambers was carried out in summer 2008 at Curno (Northern Italy) in order to study the effects of ozone and mild water stress on poplar cuttings (Oxford clone). In this experiment direct fluorescence parameters (JIP-test) were measured in leaves from different sections of the crown (L: lower; M: medium; U: upper parts of the crown). The parameters considered were calculated at the different steps of the fluorescence transient, and include maximum quantum yield efficiency in the dark-adapted state (F(v)/F(M)); the L-band, at 100?∝?s, that expresses the stability of the tripartite system reaction centre-harvesting light complex-core antenna; the K-band, at 300?∝?s, that expresses the efficiency of the oxygen-evolving complex; the J-phase, at 2 ms, that expresses the efficiency with which a trapped exciton can move an electron into the electron transport chain from Q(A)(-) to the intersystem electron acceptors; the IP-phase, which expresses the efficiency of electron transport around the photosystem 1 (PSI) to reduce the final acceptors of the electron transport chain, i.e., ferredoxin and NADP; and finally the performance index total (PItot) for energy conservation from photons absorbed by PSII to the reduction flux of PSI end acceptors. The main results are: (i) different dynamics were observed between leaves in the lower section, whose PItot decreased over time, and those in the upper sections in which it increased, with a dynamic connected to the leaf age; (ii) ozone depressed all the considered fluorescence parameters in basal leaves of well-watered plants, while it had little or no damaging effect on medium-level or upper-section leaves; (iii) PItot and IP-phase increased in upper leaves of plants subjected to ozone stress, as well as the net photosynthesis; (iv) water stress increased PItot of leaves in all levels of the crown. The results suggest that ozone-damaged poplar plants compensate, at least partially, for the loss of photosynthesis with higher photosynthetic rates in young leaves (in the upper section of the crown), more efficient to fix carbon. 相似文献
130.
Giangaspero M Nicholas RA Hlusek M Bonfini B Osawa T Orusa R Tatami S Takagi E Moriya H Okura N Kato K Kimura A Harasawa R Ayling RD 《Tropical animal health and production》2012,44(3):395-398
Sheep flocks from Hokkaido, Iwate and Aomori, three northern prefectures of Japan, were screened for antibodies to Mycoplasma ovipneumoniae and Mycoplasma agalactiae by ELISA. Sixty four animals out of 246 (26%) were seropositive to M. ovipneumoniae, with positive results obtained from all three prefectures. None of the sera tested were serologically positive to M. agalactiae. 相似文献