Effects of gelatin sponge implantation in cancellous bone defects in dogs

Reports from oral surgeons suggested that packing bone defects with cellulose or collagen stimulated cancellous bone replacement. The study reported here was designed to evaluate a similar method for augmenting the remodeling of cancellous bone defects and graft donor sites. Gelatin sponge was implanted into bony defects created in the proximal humeral metaphysis of 5 adult Beagles after collection of cancellous bone. A similar defect created in the contralateral humerus was left unpacked. Four months after creation of the defect, the dogs were euthanatized, and the surgical sites were evaluated by use of correlated microradiographic and histologic examination. Unpacked sites had filled completely with loosely woven trabecular bone. Remodeling had not taken place in sites packed with gelatin sponge. Gelatin sponge did not elicit any reaction in the surrounding bone, but remained in situ and obstructed new bone formation. Gelatin sponge blocked rather than augmented cancellous bone replacement.     

Investigating the relationship between abomasal hairballs and perforating abomasal ulcers in unweaned beef calves.

This study investigated the relationship between abomasal hairballs (trichobezoars) and perforating abomasal ulcers in unweaned beef calves <4 mo of age. The calves enrolled in the study represented routine necropsy submissions to veterinary practitioners in western Canada and to the Western College of Veterinary Medicine. Regardless of the cause of death, each calf was examined for evidence of abomasal ulcers and hairballs. Thirty-two practitioners and the Western College of Veterinary Medicine provided 166 cases for the study; 56 died of perforating ulcers (ulcer calves), and 110 died of causes unrelated to abomasum (non-ulcer calves). The calves ranged in age from 1-90 d; 154 (92.8%) were <61 d of age. Overall, ulcer calves were 2.74 (P = 0.003) times more likely to die with an abomasal hairball than were the nonulcer calves. However, stratifying the calves into 2 age groups, young (<31 d) and old (>30 d), yielded conflicting results. While the young ulcer calves were 3.81 (P = 0.003) times more likely to have a hairball than were the young nonulcer calves, there was no statistically significant relationship (OR = 0.76, P = 0.65) between ulcer and hairballs in the older calves. The authors concluded that the relationship between hairballs and ulcers in the young calves was probably spurious, created by a Berkson's bias. It is unlikely that abomasal hairballs have a significant role in the development of fatal perforating ulcers in beef calves.     

Developmental Anomalies in Farm Animals: II. Defining Etiology

Anomalous development is a function of the genetic make-up of the fetus and the environment in which it develops. The cause of anomalous development may occasionally be obvious but more often is obscure, because of its multifactorial nature. Therefore a methodological approach is often required to define the etiology. In this paper we describe a methodological approach that first defines the incident, the genotype of the individuals involved, and the environment in which the fetus has developed. This procedure can be undertaken in a stepwise fashion and need not be followed through to the end if the etiology becomes obvious. This approach highlights the necessity to characterize the defects, to use ancillary tests, and to apply basic epidemiological methods.     

Congenital spinal stenosis and dam mortality associated with feeding moldy cereal straw

We describe herein an epidemiological investigation of the cause of a syndrome of congenital spinal stenosis (CSS) in four beef herds in western Canada. Three of the herds were affected with the syndrome in 1987, the fourth was affected in 1990. The prevalence of CSS in affected groups of calves varied from 29% (16/55) to 100% (14/14). All affected calves had congenital posterior paralysis or paresis; some calves also had one or more of the following: shortened limbs, forelimb varus deformities, superior brachygnathia, and a dome-shaped cranium. Affected calves showed focal premature closure of axial and appendicular metaphyseal growth plates. At three of the four farms most of the pregnant cows were affected with alopecia with or without pruritus in January, and 25% of the cows in one herd died during the winter. The investigation indicated that CSS was associated with feeding moldy cereal straw to pregnant beef cows during the winter. At all four farms, the cereal straw bales were thoroughly soaked by rain prior to stacking, and obvious mold was present when they were broken open for feeding. Species of both Penicillium and Fusarium were abundant within the bales. The most likely cause of the disease was a fungal mycotoxicosis, although the mycotoxin responsible was not isolated.     

A field investigation of the economic impact of respiratory disease in feedlot calves

A trial involving 512 beef calves was conducted in a commercial research feedlot to determine the effect of bovine respiratory disease (BRD) on performance parameters and carcass characteristics. Two hundred and fifty-six calves that were deemed to be “sick” (S) from BRD were allocated to 16 pens and 256 calves that were considered to be “well” (W) were allocated to another 16 pens. The outcome variables that were measured included average daily gain (ADG), daily dry matter intake (DDMI), dry matter intake to gain ratio (DM:G), BRD treatment rate, death loss, carcass traits, and net profit per pen.

The data were partitioned into several time intervals including processing (P) to day −1, day 0 to day 27, day 28 to day 55, day 56 to day 83, day 84 to day 111, day 112 to day 139, day 140 to slaughter, day 0 to slaughter (0-Slaugh), and processing to slaughter (P-Slaugh). However, the most important interval was from processing to slaughter.

For the interval P-Slaugh, there were no significant (p≥0.05) differences between the S and W groups with respect to ADG and DM:G. Also, for the interval 0-Slaugh, the DDMI was similar for both groups. There were no significant (p≥0.05) differences between the S and W groups for carcass weight, average fat, grade fat, rib eye area, marbling score, cutability estimate, or carcass grade distribution.

The BRD treatment rates in the S and W groups were 6.6% and 4.7%, respectively. The mortality rates in the S and W groups were 0.78% and 0.39%, respectively. Also, there were no deaths attributable to BRD in either group.

In the economic model, there was no significant (p≥0.05) difference between the S and W groups with respect to net profit per pen.

We conclude that this trial did not validate the concept that BRD impacts performance parameters, because a sufficient disease challenge was not present. However, this study provides several observations that will enhance the experimental design of future studies that attempt to quantify the total economic impact of BRD.

     

Influence of Cysteamine on In Vitro Maturation, In Vitro and In Vivo Fertilization of Equine Oocytes

The effect of cysteamine on in vitro nuclear and cytoplasmic maturation of equine oocytes collected by transvaginal ultrasound guided follicular aspiration was assessed. Oocytes were matured in vitro with (cysteamine group) or without (control group) cysteamine. The nuclear stage after DNA Hoechst staining, penetration rates after two different in vitro fertilization (IVF) techniques (IVF media with ionophore and Hepes buffer with heparin) and the embryo yield following oocyte intra-oviductal transfer were used as a criterion for assessing nuclear and cytoplasmic maturation, respectively. Contrary to the data described in other domestic species, there was no effect of cysteamine on in vitro nuclear maturation, IVF or in vivo embryonic development under our conditions. Ovum pick up yields (52%) and maturation rates (control group: 47% and cysteamine group: 55%) were similar to those previously reported. From 57 oocytes transferred to the oviduct in each group, the number of embryos collected was 10 (17%) in the control group and five in the cysteamine group (9%). Those two percentages were not statistically different (p > 0.05). No effect of IVF technique was seen on the success rate (6%) in each group.     

Factors Affecting the Readiness and Preparation of Replacement Heifers in Tropical Breeding Environments

The future of beef cattle production in tropical regions is uncertain because of land tenure concerns, threatening the survival of extensive management programmes in these areas of the world. The need to intensify production systems will inevitably lead to changes in the management of replacement animals in existing herds. The present review underlines the main constraints, which in the past could be resolved by the sheer numbers of animals present in beef cattle operations, with replacements reliant only on finding enough heifers to substitute the adult females destined for culling. This scenario is rapidly changing as beef cattle units in tropical regions subdivide to share out the land between as many farmers as possible. Consequently, issues such as the efforts needed to manage the pregnant cow, particularly her nutritional status and its effect on the new‐born calf, the future replacement heifer growing in the presence of the dam, weaning management and procedures required before a heifer can be introduced into a breeding programme, need to be adequately and efficiently addressed by farmers in tropical regions.     

The prevalence of bovine viral diarrhea virus infection in a population of feedlot calves in western Canada.

The prevalence of bovine viral diarrhea virus (BVDV) infection was examined in a population of 5129 recently weaned steer calves entering a large feedlot in central Saskatchewan from September to December 1991. Serum samples were collected within 24 h of arrival at the feedlot from every fifth calf processed and again 96 d postarrival. A microtiter virus isolation test was used to determine the prevalence of calves viremic with BVDV on entry to the feedlot. An enzyme-linked immunosorbent assay (ELISA) which detects antibody against glycoprotein 53 of the BVDV was used on paired sera to determine the seroconversion risk during the first 96 d in the feedlot. A virus neutralization (VN) test for BVDV was conducted on a sub-sample of paired sera to measure agreement in determination of seroconversion risk with the ELISA. A polymerase chain reaction (PCR) test which detects BVDV was used to determine if cattle were acutely viremic when treated for disease. The estimated prevalence of persistently infected calves in this population was < 0.1%. The seroconversion risk for BVDV was 27% (236/864) according to the ELISA and it varied from 0 to 63% among the 20 pens sampled. According to the VN test, the seroconversion risk for BVDV was 40% (132/327) and it varied from 0 to 100% among the 11 pens tested. The agreement between the ELISA and VN tests in seroconversion risk to BVDV was very poor (kappa = 0.15 +/- 0.039 SE). The prevalence of acute viremia in calves treated at the feedlot hospital was low at 4% (6/149).(ABSTRACT TRUNCATED AT 250 WORDS)     

Effect of time of year, weather, and the pattern of auction market sales on fatal fibrinous pneumonia (shipping fever) in calves in a large feedlot in Alberta (1985-1988).

A total of 58,885 spring-born calves entering a large commercial feedlot in southwestern Alberta were studied to examine the associations between shipping fever mortality and the pattern of calf sales at the auction markets, time of year, and weather. The observational study followed calves purchased from 42 auction markets in the 4 western provinces between September 1 and December 31 in each of the years from 1985 to 1988. Calf sales at the auction markets consistently peaked during the last week of October and the first week of November. Calves entering the feedlot in November had a risk of fatal shipping fever 2 to 8 times greater than calves entering in September or December. The pattern was the same for all 4 years, with maximum risk occurring 2 to 4 weeks after the peak time for calf sales at the markets. A number of factors could have contributed to this pattern, including changes in transport truck availability, changes in the density of calves at the markets, changes in population dynamics at the feedlot that affected feedlot crew efficiency, and weather. The finding that the risk of fatal shipping fever appears to increase significantly as the feedlot fills with calves in the fall deserves the attention of feedlot owners, so they can design their treatment strategies appropriately, and of researchers, who may gain useful knowledge about the natural history of the disease by investigating why this change in risk occurs.     

The pattern of fatal fibrinous pneumonia (shipping fever) affecting calves in a large feedlot in Alberta (1985-1988).

Data from a retrospective field study were used to describe the epidemiology of fatal fibrinous pneumonia as it affected beef calves entering a large commercial feedlot in southwestern Alberta during the fall months of y 1985 to 1988. A chute-side computer system was used to record processing and health data on 58885 calves during this period. The large annual variation (10%-57%) in the proportion of total mortality due to fibrinous pneumonia indicated that crude mortality cannot be used in epidemiological studies as a surrogate measure of fibrinous pneumonia mortality. Yearly epidemic curves for fatal fibrinous pneumonia were very similar, with a short time interval (median, 19-22 d) between arrival and fatal disease. Fully 75% of the calves that died of fibrinous pneumonia already were sick within 2 weeks of arrival. Studies of the biological, environmental, and population factors that are present before and shortly after arrival at the feedlot are needed to identify strategies for reducing the incidence of fatal fibrinous pneumonia.