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91.
Studies of inositol phospholipid-specific phospholipase C 总被引:65,自引:0,他引:65
Inositol phospholipid-specific phospholipase C is the enzyme that generates phosphoinositide-derived messenger molecules. Mammalian cells contain at least five immunologically distinct phospholipase C enzymes that appear to be separate gene products. Complete amino acid sequences of four of these isozymes have been established. The overall sequence similarity is surprisingly low for enzymes catalyzing the same chemical reaction: three of them show limited amino acid sequence similarity to each other in two narrow regions, and the fourth enzyme is completely different. The diversity in primary structure together with different regional and cellular expression of the isozymes suggests that each isozyme has a defined function in processing the physiological response of different cell types to a variety of external stimuli and that each is regulated differently. 相似文献
92.
Jaeho Choi Chun Gyeong Yoon Han-Pil Rhee Yeongkwon Son Moonsoo Cho Je ha Ryu 《Paddy and Water Environment》2016,14(2):281-288
There are more than 130 agricultural water sources that are located near wastewater treatment plants (WWTPS) in Korea. The majority of the stream flow in these farmlands is dependent on effluent from the WWTPS during the dry season. This explains the indirect or direct reuse of effluent from WWTPs. Most of the farm workers use the effluent without any additional treatment because they have a lack of knowledge regarding water reuse. In addition, insufficient consideration is given to health and hygiene safety. This study reviewed the safety issues in these farmlands. A total of 53 farmlands located near WWTPs were investigated to determine if farm workers used effluent as irrigation water on their paddy rice fields. Total coliform, fecal coliform, Escherichia coli (E. coli), and the concentration of some heavy metals in paddy water and soils were measured. Quantitative microbial and toxic risk assessment methods were used to review the safety of wastewater irrigation. E. coli concentrations were used to estimate the microbial risk of enteric disease in the paddy fields. The microbial risk was 5.9 × 10?4, which did not satisfy the minimum safety standards. Carcinogenic risk was 3.99 × 10?5 and non-carcinogenic risk was 6.34 × 10?1. These values were too high to be considered safe, even though the measurements of E. coli and some toxic metals were of short duration. 相似文献
93.
Liquid crystalline (LC) poly(ethylene terephthalate-co-2(3)-chloro-1,4-phenylene terephthalate) (50/50, mole/mole) [PECPT] was synthesized and blended with polycarbonate (PC).
LC properties of PECPT and thermal, morphological, and rheological behaviors of the PECPT/PC blend were studied. PECPT showed
the nematic LC phase and much longer relaxation time than poly(ethylene terephthalate) (PET). The apparent melt viscosity
of PECPT was one third of that of PET. An abrupt torque change was observed during the blending process due to the orientation
of LC domains. For the blends containing 10∼30 wt% of PECPT, the complex viscosities were higher than that of PC. As PECPT
content increases above 40 wt%, shear thinning was observed. The lowest complex viscosity was obtained at 40∼50 wt%. Transesterification
of PECPT and PC was confirmed by the selective chemical degradation of carbonate groups in PC. 相似文献
94.
Liquid crystalline (LC) poly(ethylene terephthalate-co-2(3)-chloro-1,4-phenylene terephthalate) [copoly(ET/CPT)] was prepared using poly(ethylene terephthalate) (PET) as a flexible
spacer, terephthalic acid (TPA), and chlorohydroquinone diacetate (CHQDA). All reactions involved in the copolymerization
were investigated using some model compounds: TPA was used for acidolysis, diphenylethyl terephthalate (DPET) for interchange
reaction between PET chains, and di-o-chlorophenyl terephthalate (DOCT) and di-m-chlorophenyl terephthalate (DMCT) for interchange reaction between PET and rigid rodlike segments. Activation energies obtained
for the acidolysis of PET with TPA and for interchange reaction of PET with DPET, DOCT, and DMCT were 19.8 kcal/mole, 26.5
kcal/mole, 60.2 kcal/mole, and 45.9 kcal/mole, respectively. This result supports that the copolymerization proceeds through
the acidolysis of PET with TPA first and subsequent polycondensation between carboxyl end group and CHQDA or acetyl end group,
which is formed from the reaction of CHQDA and TPA. Also, it was found that ester-interchange reaction can be influenced by
the steric hindrance. Copoly(ET/CPT)s obtained had ethylene acetate end groups formed from acetic acid and hydroxy ethylene
end groups and showed almost the random sequence distribution for all compositions. 相似文献
95.
Kang JH Song KH Woo JK Park MH Rhee MH Choi C Oh SH 《Plant foods for human nutrition (Dordrecht, Netherlands)》2011,66(3):298-305
Cancer prevention is effective and reduces health care costs because cancer is often a preventable disease that can be affected
by lifestyle factors. Therefore, researchers are interested in discovering natural compounds that have anticancer activities,
such as delaying the development of cancer and preventing its progression. One such natural agent is ginseng (Panax ginseng), which is traditionally used in some parts of the world as a popular remedy for various diseases including cancer. We hypothesized
that the ginsenoside Rp1, a component of ginseng, reduces cancer cell proliferation through inhibition of the insulin-like
growth factor 1 receptor (IGF-1R)/Akt pathway. We first tested the efficacy of Rp1 against human breast cancer cell lines.
Treatment with Rp1 inhibited breast cancer cell proliferation and inhibited both anchorage-dependent and -independent breast
cancer cell colony formation. In addition, treatment with 20 μM Rp1 induced cycle arrest and apoptosis-mediated cell growth
suppression. Our findings further indicated that Rp1 decreased the stability of the IGF-1R protein in breast cancer cells.
Therefore, we suggest that Rp1 has potential as an anticancer drug and that IGF-1R is an important target for treatment and
prevention of breast cancer. 相似文献
96.
MA Pozor G Zambrano J Roser R Hess S Runyon E Runcan BF Thomas D Dymock ML Macpherson MH Troedsson A Kelleman 《Reproduction in domestic animals》2014,49(3):392-402
The objective of this study was to evaluate acute endocrine effects as well as histological changes in testicular parenchyma induced by the contraceptive compound RTI‐4587‐073(l). Six miniature stallions were used in this experiment. The treatment group (n = 3) received one oral dose of 12.5 mg/kg of RTI‐4587‐073(l), and the control group (n = 3) received placebo only. The stallions' baseline parameters (semen, testicular dimensions, endocrine values) were collected and recorded for 5 weeks before treatment and for 6 weeks after treatment. Multiple blood samples were collected for endocrine analysis. Testicular biopsies were obtained before treatment, 1 day after treatment and every other week after treatment. Ultrasound exams were performed to monitor the dimensions of the stallions' testes. All stallions were castrated 6 weeks after treatment. Sperm numbers, motility and percentage of morphologically normal sperm decreased (p < 0.05), while the number of immature germ cells increased in ejaculates from treated animals (p < 0.05). Serum concentrations of inhibin and follicle‐stimulating hormone did not change. Testosterone concentrations initially transiently decreased (p < 0.05) after administration of RTI‐4587‐073(l), and increased several days later (p < 0.05). Testicular content of testosterone and estradiol 17‐β was lower in treated stallions than in control stallions on Day 1 after treatment (p < 0.05). Severe disorganization of the seminiferous tubules, significant loss of immature germ cells and complete depletion of elongated spermatids were observed in testicular biopsies obtained from treated stallions 1 day, 2 and 4 weeks after treatment. These changes were still present in the testicular samples taken from treated stallions after castration. The results of this study confirmed that RTI‐4587‐073(l) has antispermatogenic effects in stallions. Furthermore, we concluded that this compound causes acute sloughing of immature germ cells from the seminiferous tubules. RTI‐4587‐073(l) has significant but transient effects on Leydig cell function in stallions. 相似文献
97.
Measurements of the oxygen isotope ratios (18O/16O and 17O/16O) in atmospheric nitrous oxide (N2O) from La Jolla, Pasadena, and the White Mountain Research Station (elevation, 3801 meters) in California and the White Sands Missile Range in New Mexico show that N2O has a mass-independent composition. These data suggest the presence of a previously undefined atmospheric process. The La Jolla samples can be explained by a mixing between an atmospherically derived source of mass-independent N2O and biologically derived mass-dependent N2O. Possible origins of the mass-independent anomaly in N2O are discussed. 相似文献
98.
Irradiation of gaseous carbon disulfide [CS2(g)] at 313 nanometers produces a dark brown aerosol of (CS2)x. Its thermal decomposition products include disulfur (S2), carbon monosulfide (CS), and (CS)x. The photopolymerization process is accompanied by a large mass-independent isotopic fractionation of sulfur (a 5 to 10 per mil sulfur-33 excess and a 61 to 84 per mil sulfur-36 deficit). Excess sulfur-33 has been observed in several classes of meteorites. Photochemical production of (CS2)x may be important in the origin and evolution of cosmochemical environments such as the presolar nebula, meteorites, asteroids, and planetary atmospheres. 相似文献
99.
PCB dechlorination in Hudson River sediment 总被引:1,自引:0,他引:1
100.
Woo HA Chae HZ Hwang SC Yang KS Kang SW Kim K Rhee SG 《Science (New York, N.Y.)》2003,300(5619):653-656
The active-site cysteine of peroxiredoxins is selectively oxidized to cysteine sulfinic acid during catalysis, which leads to inactivation of peroxidase activity. This oxidation was thought to be irreversible. However, by metabolic labeling of mammalian cells with 35S, we show that the sulfinic form of peroxiredoxin I, produced during the exposure of cells to H2O2, is rapidly reduced to the catalytically active thiol form. The mammalian cells' ability to reduce protein sulfinic acid might serve as a mechanism to repair oxidatively damaged proteins or represent a new type of cyclic modification by which the function of various proteins is regulated. 相似文献