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61.
In order to assess the seroprevalence of bovine neosporosis with indirect fluorescent antibody test (IFAT), blood samples were collected randomly from 1063 beef and dairy cattle belonging to 12 different breeds in Northeast Hungary. Antibodies to Neospora caninum were detected in 27 (2.5%) of the animals, kept on 19 of the 42 settlements included in this survey. Since samples were collected on 50 farms, herd prevalence amounted to 38%. The percentage of cattle with seroconversion increased with age, suggesting a postnatal source of infection. The highest rate of positivity was detected in Aberdeen Angus (3.3%) and Holstein-Friesian cows (3.2%), and the lowest in Limousine (0.9%), but no breed predisposition was statistically substantiated. Neosporosis was more prevalent in dairy (3.4%) than in beef (1.9%) cattle, although the difference was not significant. Only three out of the seropositive cows, all of them Holstein-Friesians, had a history of abortion. 相似文献
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Antoine Cournoyer Annie Deschamps Liza Bau-Gaudreault Pascal Dubreuil Marie-Odile Benoit-Biancamano 《Journal of veterinary diagnostic investigation》2022,34(5):789
Glucose and trehalose are the main energy sources used by honeybees (Apis mellifera) for daily activities. However, there is no validated point-of-care method to reliably measure both sugars. We performed an analytical validation of a portable human glucometer (Accu-Chek; Roche) for glucose measurement in honeybee hemolymph compared to a reference method (GluCH, UniCel DxC 600; Beckman Coulter). We used 30 pooled hemolymph samples collected from the antennae of anesthetized honeybees and diluted 1:4 in 0.9% saline. We evaluated dilution linearity, spike recovery, and inter- and intra-assay imprecision. Glucose concentration was measured over time (2 h, 4 h, 8 h, 12 h, 1 d, 2 d, 3 d, 7 d, 21 d, 28 d) at various storage temperature (25°C, 4°C, −20°C, −80°C). The trehalose concentration was measured indirectly by trehalase hydrolyzation. Glucose concentrations measured by both instruments had a strong correlation (0.985, p < 0.0001) and a bias of −7.33 mmol/L (±1.96SD: 13.70 to −28.36), with linear agreement at <20 mmol/L (physiologic value: 100 mmol/L). The accuracy of the glucometer decreased at >20 mmol/L. Recovery of 115–130% of diluted spikes indicated good specificity. Inter- and intra-assay imprecision were 2.50% and 2.21%, respectively. Glucose concentrations fluctuated in stored samples dependent on time and temperature; however, glucose concentrations were constant with storage at −80°C for ≥28 d. The Accu-Chek glucometer is an adequate instrument to measure honeybee glucose concentration in hemolymph diluted with 0.9% NaCl, with good accuracy and precision at <20 mmol/L. Hemolymph storage at −80°C is suitable for long-term conservation of glucose. 相似文献
64.
Anne Villemey William E. Peterman Murielle Richard Annie Ouin Inge van Halder Virginie M. Stevens Michel Baguette Philip Roche Frédéric Archaux 《Landscape Ecology》2016,31(7):1629-1641
Context
Anthropogenic activities readily result in the fragmentation of habitats such that species persistence increasingly depends on their ability to disperse. However, landscape features that enhance or limit individual dispersal are often poorly understood. Landscape genetics has recently provided innovative solutions to evaluate landscape resistance to dispersal.Objectives
We studied the dispersal of the common meadow brown butterfly, Maniola jurtina, in agricultural landscapes, using a replicated study design and rigorous statistical analyses. Based on existing behavioral and life history research, we hypothesized that the meadow brown would preferentially disperse through its preferred grassy habitats (meadows and road verges) and avoid dispersing through woodlands and the agricultural matrix.Methods
Samples were collected in 18 study landscapes of 5 × 5 km in three contrasting agricultural French regions. Using circuit theory, least cost path and transect-based methods, we analyzed the effect of the landscape on gene flow separately for each sex.Results
Analysis of 1681 samples with 6 microsatellites loci revealed that landscape features weakly influence meadow brown butterfly gene flow. Gene flow in both sexes appeared to be weakly limited by forests and arable lands, whereas grasslands and grassy linear elements (road verges) were more likely to enhance gene flow.Conclusion
Our results are consistent with the hypothesis of greater dispersal through landscape elements that are most similar to suitable habitat. Our spatially replicated landscape genetics study allowed us to detect subtle landscape effects on butterfly gene flow, and these findings were reinforced by consistent results across analytical methods.65.
Because of their highly polymorphic shell patterns, Cepaea land snails have been the subject of numerous studies in ecological genetics. Here, we investigated the spatial structure
of polychromatism in Cepaea hortensis in agricultural landscapes with zones from low to high hedgerow densities. Our main purpose was to search for a relationship
between landscape composition and spatial structuring of chromatism. Despite significant spatial heterogeneity in the three
landscapes sampled, only the high hedgerow density landscape showed a significant spatial structuring of shell polymorphism.
In order to understand this result, an investigation of daily movement patterns in relation to habitat form was carried out
on a mark-release experiment under semi-artificial conditions. This experiment revealed a strong influence of a linear corridor
on snail dispersal. In the field, spatial heterogeneity of shell polymorphism, related to the effects of genetic drift, was
shaped by restricted dispersal in narrow corridors. In the more enclosed one, i.e. where hedgerow density was the highest,
the significant spatial structure we detected involved a balance between local genetic drift and environmentally mediated
gene flow. This isolation-by-distance pattern resulted from direct gene exchange through fields between neighbouring populations.
When applying landscape distances based on hedgerow length, no significant spatial correlation with polychromatism was found.
In the more fragmented sites, random genetic drift seemed to be the prevailing force and, at the scale of the whole sampled
area, selective pressures potentially interfere with these genetic drift-dispersal events. 相似文献
66.
Rock outcrops in the boreal forest of Québec can show either of two different states: a forested state with >25% tree cover,
and an unforested state (<25% tree cover). We tested three different hypotheses that might explain the co-occurrence of forested
and unforested rock outcrops: (1) differences in bedrock geology, with unforested outcrops associated to bedrock types inimical
to tree growth; (2) unforested outcrops as recently disturbed sites undergoing secondary succession towards a forested state;
(3) unforested outcrops as an alternative stable state to forested outcrops, induced by post-fire regeneration failure. Digitized
forest inventory maps were used along with bedrock geology maps and time-since-fire maps to compare forested and unforested
outcrops for bedrock geology type and date of the last fire. Field surveys were conducted on 28 outcrops (14 forested, 14
unforested) to gather information regarding tree species composition and site characteristics (thickness of the organic matter
layer, percent cover of lichens, mosses and ericaceous shrubs). None of the three hypotheses explain the co-occurrence of
forested and unforested rock outcrops in the boreal forest of Québec. Both outcrop types occur on the same bedrock geology
types. Unforested outcrops are not recently disturbed sites in early-successional states, as no clear distinction could be
made in tree species composition and date of the last fire between the two outcrop types. Forested and unforested outcrops
are not alternative stable states, as unforested outcrops are unstable and cannot maintain themselves through time in the
prolonged absence of fire. Hence, unforested rock outcrops could be viewed as degraded, diverging post-fire types maintained
by the late Holocene disturbance regime, characterized by high fire frequencies. 相似文献
67.
Grassmann J Hippeli S Vollmann R Elstner EF 《Journal of agricultural and food chemistry》2003,51(26):7576-7582
The essential oil from Pinus mugo (PMEO) was tested on its antioxidative capacity. For this purpose, several biochemical test systems were chosen (e.g., the Fenton System, the xanthine oxidase assay, or the copper-induced oxidation of low-density lipoprotein (LDL)). The results show that there is moderate or weak antioxidative activity when tested in aqueous environments, like in the Fenton system, xanthine oxidase induced superoxide radical formation, or in the HOCl driven fragmentation of 1-aminocyclopropane-1-carboxylic acid (ACC). In contrast, when tested in more lipophilic environments (e.g., the ACC-cleavage by activated neutrophils in whole blood) the PMEO exhibits good antioxidative activity. PMEO does also show good antioxidative capacity in another lipophilic test system (i.e., the copper induced oxidation of LDL). Some components of PMEO (i.e., Delta(3)-carene, camphene, alpha-pinene, (+)-limonene and terpinolene) were also tested. As the PMEO, they showed weak or no antioxidant activity in aqueous environments, but some of them were effective antioxidants regarding ACC-cleavage by activated neutrophils in whole blood or copper-induced LDL-oxidation. Terpinolene, a minor component of PMEO, exhibited remarkable protection against LDL-oxidation. 相似文献
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Denis M Henrique E Chidaine B Tircot A Bougeard S Fravalo P 《Veterinary microbiology》2011,154(1-2):163-170
Sows have been identified as a source of Campylobacter contamination in piglets. We carried out a one-year study, in 2008, at 53 farrow-to-finish farms in Brittany, France, to determine the proportion of sows excreting Campylobacter. We also determined the genotypes of the Campylobacter isolates. Moreover, Generalized Estimating Equations including repeated effects were used to assess the association between management practices and farm characteristics, and risk of Campylobacter shedding by sows. Per farm, 10 feces samples from sows were collected from selected sites (maternity, service area, gestation area) on the farms. Campylobacter isolates were identified by PCR and typed by PFGE. Campylobacter was detected in 25.1% of the 530 samples from sows, and 67% of the 53 pig farms had at least one positive sample (of 10 taken). All the Campylobacter isolates belonged to the Campylobacter coli species. They displayed a very high level of genetic diversity, also inside farms and few genotypes were common to several farms. Warmer months, large farms, and individual housing for sows were identified as risk indicators of Campylobacter shedding by sows. A short delay between sampling and treatment of the samples should be considered, to improve the detection of the bacterium in the feces samples. 相似文献