Central nervous system signs in chickens caused by a new avian reovirus strain: a pathogenesis study

The present study describes the pathogenesis of infection of chicks with a new avian reovirus strain, belonging to the so-called enteric reovirus strains (ERS) that is capable of causing central nervous system signs in SPF white leghorns. After intramuscular (IM) or oral inoculation birds were either observed for clinical signs or sacrificed for macroscopic, histological and virological examination for 21 days. Virus isolation was performed on the brain, leg muscle, hock joint, liver and spleen. For the detection of viral antigen the immunohistochemistry (IHC) technique was performed on the caudal part of the cerebrum, spinal cord including spinal ganglia and right N. Ischiadicus. High mortality (79% in 7 days) was seen in birds that were inoculated IM. Survivors were depressed and stayed small until the end of the experiment. One bird had tremor and showed torticollis at 9 days after IM inoculation. Birds that were inoculated orally were depressed from day 4 and stayed small until the end of the experiment. One bird showed a torticollis at 10 days after inoculation. After both IM and oral inoculation ERS was isolated from the brain between 3 and 10 days after inoculation. Other examined organs were positive for virus isolation from day 1 or 5 until day 21. IHC revealed viral antigen positive cells in the Plexus chorioideus (plexus epithelial cells or cells within the underlying connective tissue) and in a spinal ganglion. The results indicate that the pathogenesis of ERS infection in chickens bears some resemblance with that of the mammalian reoviruses serotype 1 in mice.     

First report of Seville root-knot nematode (Meloidogyne hispanica) infecting tobacco in the Brazil

Journal of Plant Diseases and Protection - Tobacco (Nicotiana tabacum L.) is one of the major nonfood crops in the Southern of Brazil. During 2019/2020 and 2020/2021 crop seasons, Root Knot...     

Improving wood volume predictions in dry tropical forest in the semi-arid Brazil

The volumetric variability of dry tropical forests in Brazil and the scarcity of studies on the subject show the need for the development of techniques that make it possible to obtain adequate and accurate wood volume estimates. In this study, we analyzed a database of thinning trees from a forest management plan in the Contendas de Sincorá National Forest, southwestern Bahia State, Brazil. The data set included a total of 300 trees with a trunk diameter ranging from 5 to 52 cm. Adjustments, validation and statistical selection of four volumetric models were performed. Due to the difference in height values for the same diameter and the low correlation between both variables, we do not suggest models which only use the diameter at breast height (DBH) variable as a predictor because they accommodate the largest estimation errors. In comparing the best single entry model (Hohenald-Krenn) with the Spurr model (best fit model), it is noted that the exclusion of height as a predictor causes the values of 136.44 and 0.93 for Akaike information criterion (AIC) and adjusted determination coefficient (R2 adj), which are poorer than the second best model (Schumacher-Hall). Regarding the minimum sample size, errors in estimation (root mean square error (RMSE) and bias) of the best model decrease as the sample size increases, especially when a larger number of trees with DBH≥15.0 cm are randomly sampled. Stratified sampling by diameter class produces smaller volume prediction errors than random sampling, especially when considering all trees. In summary, the Spurr and Schumacher-Hall models perform better. These models suggest that the total variance explained in the estimates is not less than 95%, producing reliable forecasts of the total volume with shell. Our estimates indicate that the bias around the average is not greater than 7%. Our results support the decision to use regression methods to build models and estimate their parameters, seeking stratification strategies in diameter classes for the sample trees. Volume estimates with valid confidence intervals can be obtained using the Spurr model for the studied dry forest. Stratified sampling of the data set for model adjustment and selection is necessary, since we find significant results with mean error square root values and bias of up to 70% of the total database.     

Meta-analysis of fungicide efficacy on soybean target spot and cost–benefit assessment

Target spot of soybean has spread in Brazil, the southeastern United States and Argentina in the last decade. A collaborative network of field Uniform Fungicide Trials (UFT) in Brazil was created in 2011 to study the target spot control efficacy of fungicides, including azoxystrobin + benzovindiflupyr (AZ_BF), carbendazim (CZM), fluxapyroxad + pyraclostrobin (FLUX_PYRA), epoxiconazole + FLUX_PYRA (EPO_FLUX_PYRA), mancozeb (MZB) and prothioconazole + trifloxystrobin (PROT_TRIF). Network meta-analysis was used to conduct a quantitative synthesis of UFT data collected from 2012 to 2016 and to evaluate the effects of disease pressure (DP, low ≤ 35% target spot severity in the nontreated control < high) and year of experiment on the overall mean efficacy and yield response to each of the tested fungicides. Based on mean percentage control of target spot severity, the tested fungicides fall into three efficacy groups (EG): high EG, FLUX_PYRA (76.2% control relative to the nontreated control) and EPO_FLUX_PYRA (75.7% control); intermediate EG, PROT_TRIF (66.5% control) and low EG, MZB (49.6% control), AZ_BF (46.7% control) and CZM (32.4% control). DP had a significant effect on yield response. At DP_Low, the highest response was due to PROT_TRIF (+342 kg ha⁻¹, +12.8%) and EPO_FLUX_PYRA (+295.5 kg ha⁻¹, +11.2%), whereas at DP_High, EPO_FLUX_PYRA and FLUX_PYRA outperformed the other treatments, with yield responses of 503 kg ha⁻¹ (+20.2%) and 469 kg ha⁻¹ (+19.1%), respectively. The probability of a positive return on fungicide investment ranged from 0.26 to 0.56 at DP_Low and from 0.34 to 0.66 at DP_High.     

苎麻苗期封行期植株营养诊断及调节施肥研究

以“养分指数”为依据对新栽麻和成龄麻苗期、封行期进行植株养分平衡营养诊断,结果表明,各时期养分元素障碍因子对产量与品质的限制程度依次为(1)苗期新栽麻B>Fe>Cu>S,成龄麻N>B>P>Fe>Cu>S;(2)封行期基础肥力较好的土壤上新栽麻Mg,B>Zn>K>Ca,成龄麻Mg,B>Zn>N,P>K>Ca;基础肥力较差的土壤上新栽麻与成龄麻Mg,B>Zn>N,P>K>Ca>     

Assessment of chromium tripicolinate supplementation and dietary energy level and source on growth, carcass, and blood criteria in growing pigs

Two experiments were conducted to evaluate potential interactive effects of supplemental Cr and dietary energy supply in growing pigs. Experiment 1 used 36 individually penned barrows, 25 to 65 kg, in a 2 x 3 factorial arrangement of supplemental Cr (0 or 200 ppb) and energy level (70, 80, or 90% of ME requirement). A corn-soybean meal basal diet was designed to supply all protein, mineral, and vitamin needs and 70% of the estimated ME need at 70% of ad libitum feed intake. Additional energy to 80% or 90% of the ME requirement was provided by a cornstarch/corn oil blend. In Exp. 2, 30 individually penned barrows, 23 to 68 kg, were used in a 2 x 4 factorial arrangement of supplemental Cr (0 or 200 ppb) and added energy source (none, cornstarch, corn oil, or choice white grease) with basal diets identical to Exp. 1. The various energy sources were added to 90% of the ME requirement. In both experiments, growth data were collected over a 50-d period and pigs were killed at 70.1 kg. Increasing energy levels increased (linear, P < 0.01) ADG, average backfat thickness, 10th rib backfat thickness, and cooler shrink and decreased (linear, P < 0.01) longissimus muscle area in Exp. 1. Carcass composition increased (linear, P < 0.01) in lipid and decreased in protein, water, and protein:lipid ratio in response to increasing ME levels. Similar results were observed in Exp. 2 in response to added energy, regardless of the energy source used. In response to ME, linear increases (P < 0.05) in plasma insulin concentration before feeding and after feeding were observed in Exp. 1. In Exp. 2, plasma insulin concentration was lower for the basal diet before feeding (P < 0.05) and higher for the starch diet after feeding (P < 0.01); insulin:glucose ratio increased (P < 0.01) after feeding for starch compared to oil and fat. No consistent effect of Cr or Cr x ME level on performance or carcass was observed (P > 0.10) in these experiments. Similarly, no Cr effect or Cr x ME interaction (P > 0.10) was observed in plasma glucose or insulin levels. Dietary energy levels markedly affected growth criteria in growing pigs (23 to 68 kg) in these experiments, as anticipated, but supplemental Cr was without effect on performance or carcass responses.