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排序方式: 共有109条查询结果,搜索用时 15 毫秒
1.
Weak activity of UDP-glucuronosyltransferase toward Bisphenol analogs in
mouse perinatal development
Risa YABUSAKI Hidetomo IWANO Sumito TSUSHIMA Nanako KOIKE Naoko OHTANI Kentaro TANEMURA Hiroki INOUE Hiroshi YOKOTA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1479-1484
Bisphenol A (BPA) is a widely used industrial chemical that disrupts endocrine function.
BPA is an endocrine disrupting chemical (EDC) that has been demonstrated to affect
reproductive organ development, brain development, metabolic disease and post-natal
behavior. Accordingly, Bisphenol analogs, Bisphenol F (BPF, bis (4-hydroxyphenyl) methane)
and Bisphenol AF (BPAF, 4,4-hexafluoroisopropylidene) diphenol) are used as replacements
for BPA. BPA is mainly metabolized by UDP-glucuronosyltransferase (UGT), UGT2B1, but this
effective metabolizing system is weak in the fetus. In the present study, we demonstrated
that hepatic UGT activity toward BPAF was very weak, in comparison with BPA and BPF, in
the fetus, pups and dams. Conversely, hepatic UGT activity toward BPF was very weak in the
fetus and newborn pups, and was increased to the same level as BPA post-partum. In
conclusion, BPAF possibly tends to accumulate in the fetus, because of weak metabolism
during the perinatal period, suggesting that the metabolism of individual Bisphenol
analogs requires assessment to properly gauge their risks. 相似文献
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Azusa SOMEYA Ryoko FUKUSHIMA Michiko YOSHIDA Yasuyuki TANAHASHI Tangmunkhong PRAPEUK Reiko IIZUKA Hiroshi HIRAMI Atsushi MATSUDA Shunichi TAKAHASHI Goro KURITA Takashi KIMURA Misuzu SEO Masayuki FUNABA Yoshii NISHINO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(8):1157-1160
6.
Taguchi H Watanabe S Temmei Y Hirao T Akiyama H Sakai S Adachi R Sakata K Urisu A Teshima R 《Journal of agricultural and food chemistry》2011,59(8):3510-3519
Shrimp and crab are well-known as allergenic ingredients. According to Japanese food allergy labeling regulations, shrimp species (including prawns, crayfishes, and lobsters) and crab species must be differentially declared when ≥10 ppm (total protein) of an allergenic ingredient is present. However, the commercial ELISA tests for the detection of crustacean proteins cannot differentiate between shrimp and crab. Therefore, two methods were developed to discriminate shrimp and crab: a shrimp-PCR method with postamplification digestion and a crab-PCR method that specifically amplifies a fragment of the 16S rRNA gene. The sensitivity and specificity of both PCR methods were verified by experiments using DNA extracted from 15 shrimp species, 13 crab species, krill, mysid, mantis shrimp, other food samples (cephalopod, shellfish, and fish), incurred foods, and commercial food products. Both PCR methods could detect 5 pg of DNA extracted from target species and 50 ng of genomic DNA extracted from incurred foods containing 10 ppm (μg/g) total protein of shrimp or crab. The two PCR methods were considered to be specific enough to separately detect species belonging to shrimp and crab. Although false-positive and false-negative results were obtained from some nontarget crustacean species, the proposed PCR methods, when used in conjunction with ELISA tests, would be a useful tool for confirmation of the validity of food allergy labeling and management of processed food safety for allergic patients. 相似文献
7.
Molla Rahman Shaibur Nobuyuki Kitajima Reiko Sugawara Toshihito Kondo S. M. Imamul Huq Shigenao Kawai 《Journal of plant nutrition》2013,36(2):333-353
The experiment was carried out to investigate the effects of arsenic (As) on the physiological and mineralogical properties of barley (Hordeum vulgare L. cv. ‘Minorimugi’). The plants were grown in nutrient solution treated with 0, 6.7, 33.5, and 67 μ M As (0, 0.5, 2.5, and 5 ppm As, respectively) in the phytotron. Dry matter yield of shoots and roots decreased significantly with the As treatments, indicating that barley plants are As-sensitive and As-toxicity depends on the As concentration in the rooting medium. Necrosis in older leaves and chlorosis symptoms (whitish color) in the fully developed young leaves were observed at the 33.5 and 67 μ M As treatments. Arsenic concentration, accumulation, and translocation increased with the increase of As concentration in the rooting medium. Arsenic was mostly concentrated in roots and a little amount was moved to shoots, indicating that As was not easily translocated to shoots of barley seedlings. Concentrations and accumulations of phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), manganese (Mn), zinc (Zn), and copper (Cu) decreased significantly in shoots for 33.5 and 67 μ M As treatments as compared to the 0 μ M As treatment. Concentrations of P, K, Ca, Mg, Mn, and Cu decreased in roots, but Zn concentration increased in roots at 67 μ M As treatment. Accumulations of P, K, Ca, Mg, Mn, Zn, and Cu in roots also decreased significantly at 67 μ M As treatment. Accumulation of P and the cations showed negative relationship with As. Concentration of Fe decreased in shoots at 33.5 and 67 μ M As treatments where chlorosis was induced in the young leaf but increased in roots at 33.5 and 67 μ M As treatments. It was suggested that As might induce iron (Fe)-chlorosis in the plants. Among the micronutrients, Fe translocation was more affected than others by As. Phytosiderophore (PS) accumulation in roots, which is a symptom of Fe-deficiency in grasses, did not change significantly between 0 and 33.5 μ M As treatments; indicating that As-induced chlorosis did not enhance PS accumulation in roots and decreased due to As-toxicity at 67 μ M As treatment. 相似文献
8.
Muneta Y Kikuma R Uenishi H Hoshino T Yoshihara K Tanaka M Hamashima N Mori Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(3):269-275
A pig interleukin-21 (IL-21) cDNA was successfully cloned and sequenced from porcine peripheral blood lymphocytes (PBL) stimulated with 10 microg/ml concanavalin A (ConA), 10 microg/ml phytohemagglutinin P (PHA), 50 ng/ml phorbol 12-myristate 13-acetate (PMA), and 0.5 microg/ml anti-porcine CD3 antibody for 48 hr. The open reading frame of the porcine IL-21 cDNA is 459 base pairs in length and encodes 152 amino acids. The predicted amino acid sequence of the porcine IL-21 shows 86.2%, 77.7%, and 58.4% identity to the bovine, human, and murine IL-21, respectively. The porcine IL-21 gene was mapped to porcine chromosome 8 (8q22-->q23) by means of fluorescence in situ hybridization and radiation hybrid mapping, where the porcine IL-2 gene had been mapped nearby. The recombinant porcine mature IL-21 expressed by E. coli induced dose-dependent proliferation and IFN-gamma production from a human NK cell line, NK0. The porcine IL-21 identified in this study will be helpful for the enhancement of innate immune responses of pigs. 相似文献
9.
Shimizu E Kato H Nakagawa Y Kodama T Futo S Minegishi Y Watanabe T Akiyama H Teshima R Furui S Hino A Kitta K 《Journal of agricultural and food chemistry》2008,56(14):5521-5527
A novel type of quantitative competitive polymerase chain reaction (QC-PCR) system for the detection and quantification of the Roundup Ready soybean (RRS) was developed. This system was designed based on the advantage of a fully validated real-time PCR method used for the quantification of RRS in Japan. A plasmid was constructed as a competitor plasmid for the detection and quantification of genetically modified soy, RRS. The plasmid contained the construct-specific sequence of RRS and the taxon-specific sequence of lectin1 (Le1), and both had 21 bp oligonucleotide insertion in the sequences. The plasmid DNA was used as a reference molecule instead of ground seeds, which enabled us to precisely and stably adjust the copy number of targets. The present study demonstrated that the novel plasmid-based QC-PCR method could be a simple and feasible alternative to the real-time PCR method used for the quantification of genetically modified organism contents. 相似文献
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