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ABSTRACT: The low salt extract from scallop striated adductor muscle contained extra proteins in addition to sarcoplasmic proteins and was turned into a gel upon standing or immediately by the addition of Ca2+ . The amount of extra protein, which was composed of a large amount of actin and a small amount of myosin, decreased with a decrease in adenosine triphosphate (ATP) content in the muscle during storage at 5°C. Actin was easily extracted from scallop myofibrils in low salt solution with ATP at 1 mM or above. Furthermore, ATP was required to induce the gelation of the low salt extract. A visual observation of the gelation of low salt extract is therefore a simple and easy method to inspect prerigor state of scallop adductor muscle. 相似文献
34.
Hideyuki KANEMOTO Rei MORIKAWA James Kenn CHAMBERS Koichi KASAHARA Yasuko HANAFUSA Kazuyuki UCHIDA Koichi OHNO Hiroyuki NAKAYAMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(6):715-719
A Pomeranian dog, 1 year- and 8 month-old neutered female, was presented with persistent
respiratory distress and recurrent generalized demodicosis. Physical examination revealed
cyanosis, rough respiratory sounds, multifocal alopecia and dermal erosions on the dorsal
side of the forelimbs, perineal area and skin around the eyes. A severe diffuse
interstitial lung pattern was observed on thoracic radiographs. The blood examination
revealed neutrophilia and hypoglobulinemia. Serum immunoglobulin concentrations of IgG and
IgA were low. Histopathological examination revealed severe diffuse interstitial pneumonia
with Pneumocystis carinii infection. Severe lymphoid depletion was
observed in the spleen and other organs with lymphoid follicles consisted mainly of
CD3-positive T cells and few cells of B-cell lineage. B-cell hypoplasia with subsequent
antibody deficiency was suspected. 相似文献
35.
Rieko OKAME Keiko NAKAHARA Yumiko KATO Makoto BANNAI Noboru MURAKAMI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(6):661-668
It has been suspected that in comparison with glucose or fatty acids, the levels of amino
acids may readily change with different forms of exercise. In the present study, we
measured the concentrations of amino acids, glucose, triglycerides, total protein and
total cholesterol in the blood and/or cerebrospinal fluid (CSF) of rats subjected to
forced running exercise on a treadmill, and voluntary running exercise using a wheel, with
a constant running distance of 440 m. Rats that performed no running and rats subjected to
immobilization stress were used as controls. We observed a few significant changes in the
levels of plasma glucose, triglycerides, total protein and total cholesterol in all
groups. Whereas, plasma amino acid levels were significantly changed by exercise and
stress, especially during the light period. The plasma levels of many amino acids were
specifically increased by forced running; some were decreased by immobilization stress.
Few amino acids showed similar changes in their levels as a result of voluntary running.
In addition, there was a significant difference in the degree of amino acid imbalance
between blood and CSF. These results provide the first information on changes in levels of
amino acids in plasma and CSF resulting from forced and voluntary exercises. 相似文献
36.
本文综述了压电晶体化学传感器在气相、液相物质和生物学上的应用,介绍了压电晶体化学传感器的新型装置(如传感器阵列)、新型敏感材料. 相似文献
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Yuko TOISHI Nobuo TSUNODA Masaaki TAGAMI Hiromitsu HASHIMOTO Fumiki KATO Tsukasa SUZUKI Kentaro NAGAOKA Gen Watanabe Shota TOKUYAMA Kiyoshi OKUDA Kazuyoshi TAYA 《Journal of Equine Science》2013,24(3):47-51
Evaluation of a new chemiluminescent enzyme immunoassay, the PATHFAST assay system
(PATHFAST), for measurement of circulating progesterone in mares was performed. Five mares
at the mid-luteal stage were administrated a single i.m. injection of prostaglandin F2α
analog (PGF2α; cloprostenol 250 μg/ml), and then blood samples were collected from the
jugular vein at 0, 15, 30 and 45 min, at one-hour intervals until 24 and at 48 hr via a
catheter in the jugular vein. To monitor the physiological changes in circulating
progesterone in mares after induced luteolysis, concentrations of progesterone in whole
blood and serum samples were measured by PATHFAST. In addition, concentrations of
progesterone in serum samples measured by PATHFAST were compared with those measured by
radioimmunoassay (RIA) and enzyme immunoassay (EIA). Using PATHFAST, the serum
concentrations of progesterone in mares correlated highly with those of whole blood
samples (r=0.9672, n=88). The serum concentrations of progesterone as measured by PATHFAST
correlated well with RIA (r=0.9654, n=88) and EIA (r=0.9323, n=112). An abrupt decline in
circulating progesterone in whole blood samples was observed within 2 hr (50%), followed
by a gradual decline until 48 hr later. The results for progesterone in whole blood
samples correlated highly with those in serum samples, and the declining pattern
paralleled that of the serum samples. These results demonstrated that PATHFAST is useful
in the equine clinic as an accurate diagnostic tool for rapid assay of progesterone within
26 min, using unextracted whole blood. 相似文献
40.
甜瓜果实中乙烯生产量及一些相关性状的遗传分析 总被引:1,自引:1,他引:0
为改进甜瓜货架期,利用成熟果实乙烯释放量及变化速率存在显著差异的3个甜瓜变种为亲本,配制了皇后×9-8(非跃变类型材料间)和皇后×Charentais(非跃变类型与跃变类型材料间)两个组合,对各组合F1,F2及回交世代BC果实中乙烯的生成变化及果实脱落性、外果皮迅速黄化等相关性状的遗传变化进行了分析。结果表明,甜瓜果实中乙烯生成量为不完全显性遗传,受两对以上基因控制。在皇后×Charentais组合的F1中,内源乙烯含量在采收1d后表现出急剧升高,同时伴随着外果皮迅速黄化及果柄脱落的发生,根据这些性状在F2及分别与父母本回交世代中的不同分离比率指出,乙烯的跃变类型、外果皮迅速黄化及果柄脱落在甜瓜果实中为显性遗传。 相似文献