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This study was conducted to evaluate whether cooled floor pads combined with chilled drinking water could alleviate negative impacts of heat stress on lactating sows. Thirty sows (Landrace × Yorkshire, Parity = 1 to 6) were housed in individual farrowing stalls in two rooms with temperatures being controlled at 29.4°C (0700–1900 hours) and 23.9°C (1900–0700 hours). Sows in one room (Cool), but not in the other room (Control) were provided cooled floor pads (21–22°C) and chilled drinking water (13–15°C). Behavior of sows (15 sows/treatment) was video recorded during farrowing, and days 1, 3, 7, 14, and 21 after farrowing. Videos were viewed continuously to register the birth time of each piglet, from which total farrowing duration and birth intervals were calculated. The number of drinking bouts and the duration of each drinking bout were registered for each sow through viewing videos continuously for 2 h (1530–1730 hours) each video-recording day. Postures (lying laterally, lying ventrally, sitting, and standing) were recorded by scanning video recordings at 5-min intervals for 24 h each video-recording day, and time budget for each posture was calculated. Rectal temperature and respiration rate were measured for all sows the day before and after farrowing, and then once weekly. Sow and litter performance was recorded. Data were analyzed using the Glimmix procedure of SAS. The cooling treatment did not affect sow behavior or litter performance. Sows in the Cool room had lower rectal temperature (P = 0.03) and lower respiration rate (P < 0.001), consumed more feed (P = 0.03), tended to have reduced weight loss (P = 0.07), and backfat loss (P = 0.07) during lactation than sows in the Control room. As lactation progressed, sows increased drinking frequency (P < 0.001) and time spent lying ventrally (P < 0.0001), standing (P < 0.001), and sitting (P < 0.0001), and decreased time spent lying laterally (P < 0.0001) in both Cool and Control rooms. While cooled floor pads combined with chilled drinking water did not affect sow behavior, they did alleviate heat stress partially, as indicated by decreased rectal temperature, respiration rate, weight, and backfat loss, and increased feed intake in lactating sows.  相似文献   
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In Exp. 1 two groups of 18 sows were used to evaluate the effects of supplemental dietary fat on sow and litter performance and milk production and composition. Sows were provided ad libitum access to either a corn-soybean meal (control) diet or a similar diet containing 10% tallow. Feed intake, ME intake, and milk yield did not differ (P > .10) between treatments. The percentage of solids in milk was greater (P < .05) for sows fed the tallow diet, due to an increase (P < .05) in the fat and ash content. Compared with percentages of fatty acids in milk of sows fed the control diet, the percentages of C10:0, C14:0, C16:0, C16:1, and C18:3 fatty acids were lower (P < .05) and the percentages of C18:0 and C18:1 fatty acids were higher in milk of sows fed tallow diets (P < .05). In Exp. 2, 30 sows were fed diets similar to those fed in Exp. 1, and the effects of a tallow diet on pig carcass composition at weaning were determined. Litter size was standardized to 10 pigs. There were no differences (P > .10) in ADFI of sows. Daily ME intake was greater for sows fed tallow than for control sows during wk 2 (P < .05), wk 3 (P < .10), and the entire lactation (P < .05) period. Litter weaning weight was greater (P < .05) for pigs from sows fed tallow diets than for pigs from control sows. Pigs from tallow-fed sows had greater carcass fat weight and fat percentages (P < .05) and lower water and protein percentages (P < .05). These data indicate that the increased fat content of milk from sows fed tallow diets resulted in an increased weight gain for litters nursing these sows. The composition of the increased weight gain is almost exclusively fat.  相似文献   
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Toxigenic strains of Pasteurella multocida were readily differentiated from non-toxigenic strains by an agarose overlay method using bovine turbinate cells or bovine lung cells. Cells which were young and densely confluent were best suited to this assay. The incubation period required to distinguish toxigenic strains was dependent on the confluence of the monolayers, which was affected by the seeding rate, cell passage level and growth time prior to overlay. The agarose overlay method correctly identified 11 of 11 reference strains of Pasteurella multocida, and visible cytotoxic changes were present in the monolayers after 48 to 65 h. Outbreaks of the enzootic form of atrophic rhinitis in 2 New South Wales piggeries were associated with the isolation of toxigenic type D strains of P. multocida.  相似文献   
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Triplets in red deer (Cervus elaphus)   总被引:1,自引:0,他引:1  
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