Carotenoid diversity in cultivated citrus is highly influenced by genetic factors

Citrus fruits are complex sources of carotenoids with more than 100 kinds of pigments reported in this genus. To understand the origin of the diversity of carotenoid compositions of citrus fruit, 25 genotypes that belong to the 8 cultivated Citrus species were analyzed. Juice extracts of mature fruit were analyzed by high-performance liquid chromatography using a C30 column. The 25 citrus genotypes presented different carotenoid profiles with 25 distinct compounds isolated. Statistical analyses revealed a strong impact of genotype on carotenoid compositions. Two kinds of classifications of genotypes were performed: on qualitative data and on quantitative data, respectively. The results showed that variability in carotenoid compositions was more interspecific than intraspecific. Two carotenoids, cis-violaxanthin and the beta-cryptoxanthin, strongly determined the classification on qualitative data, which was also in agreement with previous citrus variety classifications. These findings provide evidence that, as for other phenotypical traits, the general evolution of cultivated Citrus is the main factor of the organization of carotenoid diversity among citrus varieties. To the authors' knowledge this is the first study that links the diversity of carotenoid composition to the citrus genetic diversity. These results lead to the proposed major biosynthetic steps involved in the differential carotenoid accumulation. Possible regulation mechanisms are also discussed.     

A novel herpesvirus in 3 species of pheasants: mountain peacock pheasant (Polyplectron inopinatum), Malayan peacock pheasant (Polyplectron malacense), and Congo peafowl (Afropavo congensis)

The mountain peacock pheasant (Polyplectron inopinatum), the Malayan peacock pheasant (Polyplectron malacense), and the Congo peafowl (Afropavo congensis) are all listed as vulnerable to extinction under the International Union for Conservation of Nature Red List of Threatened Species. Here the authors report fatal infection with a novel herpesvirus in all 3 species of birds. DNA was extracted from the livers of birds with hepatocellular necrosis and intranuclear eosinophilic inclusions consistent with herpesvirus infection. Based on degenerate herpesvirus primers and polymerase chain reaction, 220- and 519-base pair products of the herpes DNA polymerase and DNA terminase genes, respectively, were amplified. Sequence analysis revealed that all birds were likely infected with the same virus. At the nucleotide level, the pheasant herpesvirus had 92% identity with gallid herpesvirus 3 and 77.7% identity with gallid herpesvirus 2. At the amino acid level, the herpes virus had 93.8% identity with gallid herpesvirus 3 and 89.4% identity with gallid herpesvirus 2. These findings indicate that the closest relative to this novel herpesvirus is gallid herpesvirus 3, a nonpathogenic virus used widely in a vaccine against Marek's disease. In situ hybridization using probes specific to the peacock pheasant herpesvirus DNA polymerase revealed strong intranuclear staining in the necrotic liver lesions of an infected Malayan peacock pheasant but no staining in normal liver from an uninfected bird. The phasianid herpesvirus reported here is a novel member of the genus Mardivirus of the subfamily Alphaherpesvirinae and is distinct from other galliform herpesviruses.     

Assessing Sources of Information for Urban Mushroom Growers in Accra,Ghana

This study assessed information sources for urban mushroom farmers in Accra, Ghana. One hundred farmers were sampled, and a structured questionnaire was administered to solicit information from the respondents. Results showed that the majority of farmers were male (71%) and most (60%) with university education. The majority (44%) had 1–5 years of experience in mushroom cultivation. Co-farmers (89.8%), the Mushroom Growers Association (79.6%), training workshops (73.5%), and the Farmers' Forum (29.6%) readily served as information sources for farmers. The research revealed that, for 52% of farmers, ignorance of information sources was a major hindrance in their quest for information.     

Manufacture of Biquara (Haemulon Plumierii - Lacepède, 1801) Fishburger with Addition of Wheat Bran

Biquara is one of the main fish species caught in the northeast coast of Brazil but presents low commercial value. Therefore, the elaboration of biquara-based products with the addition of plant bran as a source of dietary fiber should be studied as a way to add value to biquara. The objective of the present study was to evaluate the physicochemical, microbiological, and sensory aspects of fishburgers made with biquara fillet with the addition of wheat bran (WB) (0, 1, 2, and 3%). The increase in WB decreased moisture and increased protein levels, ash, fiber, carbohydrates, and energy value. In addition, the inclusion of WB increased the cooking shrinkage and decreased hardness and cohesiveness of the samples. The fishburgers with the greatest WB levels presented a reddish-yellow hue. The microbial counts of the products were below the maximum limit allowed by the Brazilian legislation. The overall acceptance of the fishburgers improved with the addition of 0 to 2% WB. Therefore, the biquara fillets have a potential for the elaboration of fishburgers with the addition of 2% WB, once the products presented suitable physicochemical composition, sensory acceptance, and hygienic sanitary control according to the current national legislation.     

A procedure for estimating the number of green mature apples in night-time orchard images using light distribution and its application to yield estimation

A procedure for estimating the number of mature apples in orchard images captured at night-time with artificial illumination was developed and its potential for estimating yield was investigated. The procedure was tested using four datasets totaling more than 800 images taken with cameras positioned at three heights. The procedure for detecting apples was based on the observation that the light distribution on apples follows a simple pattern in which the perceived light intensity decreases with the distance from a local maximum due to specular reflection. Accordingly, apple detection was achieved by detecting concentric circles (or parts of circles) in binary images obtained via threshold operations. For each dataset, after calibration of the procedure using 12 images, the estimates of the number of apples were within a few percent of the number of apples counted by visual inspection. Yield estimations were obtained via multi-linear models that used between two and six images per tree. The results obtained using all three cameras were only slightly better than those obtained using only two cameras. Using images from only one side of the tree did not worsen the results significantly. Overall, the yield estimated by the best models was within \(\pm\)10 % of the actual yield. However, the standard deviation of the yield estimation errors corresponded to ~26–37 % of the average tree yield, indicating that improvements are still needed in order to achieve accurate yield estimation at the single-tree level.     

不同豆科植物对土壤酸化的影响

以9种豆科牧草为试材,在缺磷砂土上研究施用Ca(H₂PO₄)₂后对其产酸量的影响.结果表明:Lupinus albus,Lupinus lutens和Biserrula pelecinus施磷需求量比Cicer aritimum,Vicia faba,Medicago polgmorpha,Ornithopus sativus和Trifolium subterraneum低,而Lupinus angustifolius需求量最高.当增施磷肥,使其达到中度缺磷水平时,比酸度有所增加,但进一步增施磷肥则使植株产酸量减少,在几种植物中,C.arietinum产酸量最高,B.pelecinus最低.比酸度与过量阳离子、灰碱、钙和镁等浓度有关.在不考虑施磷和植物品种的情况下,植物产酸总量与植株地上部分过量阳离子总量恰好一致;在施磷量不同的情况下,豆科植物产酸量主要由其吸收阳离子和阴离子的差异决定.     

Practical diets with essential oils of plants activate the complement system and alter the intestinal morphology of Nile tilapia

The effect of the essential oils (EOs) of peppermint, Mentha piperita L., and tea tree, Melaleuca alternifolia (Maiden & Betche) Cheel, on the haematological, biochemical, and immunological parameters and intestinal morphology of Nile tilapia, Oreochromis niloticus L., was evaluated. Fish (58.09 ± 5.87 g) were fed 100 mg/kg and 250 mg/kg of each EO and sampled on days 7, 14, 30 and 60 after starting supplementation. The haematological and biochemical parameters were not altered by the supplementation of EOs compared to the control (p > .05). With regard to the immunological parameters, the activation of the complement system of fish fed 250 mg/kg peppermint and 100 mg/kg and 250 mg/kg tea tree EOs were significantly higher compared to the control after 60 days of feeding (p < .05). The complement system plays an essential role in innate immunity and contributes significantly to the acquired immune response; thus, its activation through supplementation with EOs is promising for the formulation of nutritional additives in aquaculture. Regarding intestinal morphology, fish fed 250 mg/kg tea tree EO presented higher villus size compared to all other groups (p < .05), which represents a healthier gut. These fish present a larger intestinal surface, which can result in better absorption and utilization of the nutrients. Based on the responses found in this study, both EOs were considered promising for the formulation of feed additives for Nile tilapia.     

奥地利两块基因资源保育林内英国紫杉生态条件与保护现状比较

在奥地利及其他多数欧洲国家英国紫杉濒临灭绝,被列为濒危树种.基于不同地理位置的自然群落的比较,调查了奥地利两块基因资源保育林内英国紫杉的空间结构、更新状况及可采取的保育方法等.每块样地圆杆材分布变化较大.林地Stiwollgraben内紫杉单位面积植株总数,平均胸径和平均株高分别为492 n·hm-2, 8.8 cm and 6.3 m;Leininger Riese仅为45 n·hm-2, 16.3 cm and 7.6 m.Stiwollgraben群落紫杉长势较好,健康植株79%以上,而Leininger Riese群落的健康植株低于49%.两块样地内紫杉更新模式差异较大,与其各自的树龄动态分布相一致.1年龄幼树相比较,Stiwollgraben林地内紫杉密度为13019株/公顷,而Leininger Riese仅为1368株/公顷.Leininger Riese林地内未发现树高51-150cm的幼树,而Stiwollgraben林地内树高30-150cm的幼树鲜能见到.这种情况下,英国紫杉林的保育需要管理完善的保护区、采伐期间采取长期轮伐,同时还要防止牲畜啃食和减轻种间竞争,以此来增强红豆杉的生存能力.图4表4参41.