早春短命植物绵果荠无菌苗组织培养相关因素研究

为建立绵果荠（Lachnoloma lehmannii）无菌苗培养体系,以种子作为试验材料,对不同消毒方法的效果进行了比较,并对影响种子萌发、幼苗生长相关因素进行了探讨。结果表明,1）种子消毒适宜方法为种子流水冲洗1~2 min,70%酒精浸泡1 min,0.1% HgCl2消毒1.5 min;2）光照对种子萌发有促进作用,而幼苗的不同部位对光照有不同响应;3）种子萌发对低温有依赖性,而幼苗生长需要一定的高温;4）适宜激素对种子萌发有促进作用,而幼苗的不同部位对激素有不同反应。因此,绵果荠种子萌发和幼苗生长适合培养体系为把种子消毒后接种无激素的MS,放在变温为15 ℃/5 ℃的光照条件下培养4~5 d,萌发后转至温度为（25±1） ℃的光照条件下培养,发芽率达到90%,比对照高16%,且幼苗长势良好。     

Response of sunflower hybrids to nitrogen application grown under different agro-environments

A wide gap exists between production and consumption of vegetable oils in Pakistan. Thereby, a significant proportion (2.28 million tons) of vegetable oils is being imported at the cost of 2257 million US$. Therefore, the present study was conducted to quantify the comparative performance of various sunflower hybrids as influenced by various levels of nitrogen (N) fertilizer under different agro-environments. The experimental treatments consisted of three sunflower hybrids (Hysun33, Hysun38, and Pioneer-64A93) and five levels of N fertilizer (0, 60, 120, 180, 240 kg N ha^?1), arranged in a randomized complete block design in a split plot with four replications. The field trials were conducted for two consecutive crop seasons under three different agro-ecologies (arid, semi-arid, and sub-humid) in the province of Punjab, Pakistan. The results of the study demonstrated that the productivity of sunflower hybrids varied greatly in response to N fertilization and different ecologies. Maximum achene yield of 3177 kg ha^?1 was harvested under sub-humid environment, followed by the semi-arid one. Among the hybrids, Hysun38 excelled the other two hybrids with a production of 3083 kg ha^?1 and 41% oil contents. Generally, the productivity of hybrids increased with the increasing doses of N fertilizer. Maximum achene yield was obtained by addition of 180 kg N ha^?1. The findings of the study revealed that yield potential of Hysun-38 could be exploited by addition of N fertilizer at the rate of 180 kg N ha^?1 under sub-humid environment.     

Assessment of field evolved resistance to some broad-spectrum insecticides in cotton jassid, <Emphasis Type="Italic">Amrasca devastans</Emphasis> from southern Punjab,Pakistan

Resistance management, targeting insect pests is one of the key components in developing integrated pest management strategies. Arguably, resistance monitoring is a scientific undertaking that can support and inform resistance management tactics and strategies. To monitor the current resistance status in Amrasca devastans against conventional insecticides (deltamethrin, bifenthrin, cypermethrin, chlorpyrifos, profenofos, acephate, and methomyl) which are used by the farming community as the predominant means to control this pest. Field populations of A. devastans were collected from six different districts: Multan, Bahawalpur, Khanewal, Lahore, Dera Ghazi Khan and Muzaffargarh from Punjab in Pakistan. The adult populations tested were 11.10–92.87 times more resistant to deltamethrin, 5.87–14.11 times more to bifenthrin, 3.16–17.5 times more to cypermethrin, 2.65–36.42 times more to chlorpyrifos, 7.28–57.71 times more to profenofos, 1.65–11.13 times more to acephate and 2.55–43.31 times more to methomyl as compared to control (lab population). In our study, no to high levels of resistance were observed against pyrethroids and organophosphates. Development of resistance to these pyrethroids and organophosphates might be due to the injudicious use of these types of insecticides in field crops. This study suggests that use of these insecticides should be minimized to avoid development of resistance in A. devastans. Future studies are also recommended to use new chemistry insecticides with novel modes of action and/or insecticide mixtures that may reduce the reliance of the farming communities on these insecticides.     

A cohort study of telomere and telomerase biology in cats

OBJECTIVE: To investigate telomere lengths in tissues of domestic shorthair (DSH) cats of various ages, evaluate the relationship between telomere length and age of cats, and investigate telomerase activity in the somatic tissues of cats. SAMPLE POPULATION: Tissues obtained from 2 DSH cats and blood samples obtained from 30 DSH cats. PROCEDURE: DNA isolated from blood cells and somatic tissue samples was subjected to terminal restriction fragment (TRF) analysis to determine mean telomere repeat lengths. Protein samples were subjected to analysis by use of a telomeric repeat-amplification protocol to assess telomerase activity. RESULTS: MeanTRF values of cats ranged from 4.7 to 26.3 kilobase pairs, and there was significant telomeric attrition with increasing age of cat. Telomerase activity was not found in a wide range of normal tissues obtained from 2 cats. CONCLUSIONS AND CLINICAL RELEVANCE: Analysis of these results clearly indicates that telomeres are shorter in older cats, compared with young cats; therefore, telomeres are implicated in the aging process. The analysis of telomerase activity in normal somatic tissues of cats reveals a pattern of expression similar to that found in human tissues. IMPACT FOR HUMAN MEDICINE: Fundamental differences in the biological characteristics of telomeres and telomerase exist between humans and the other most widely studied species (ie, mice). The results reported here reveal similarities in telomere and telomerase biologic characteristics between DSH cats and humans. Hence, as well as developing our understanding of aging in cats, these data may be usefully extrapolated to aging in humans.     

CRITICAL DOSE OF NITROGEN AND PHOSPHORUS ENHANCED GROWTH,YIELD AND ALKALOID CONTENT IN WITHANIA SOMNIFERA L.

The optimum dose of nitrogen (N) and phosphorus (P) for Withania somnifera was determined by utilizing graded levels of 0, 30, 45 and 60 kg ha^?1 N and 0, 13, 26 and 40 kg ha^?1 P in two separate simple randomized pot experiments. Soil-application of 45 kg N ha^?1 and 26 kg P ha^?1 proved the best dose for increasing shoot and root length, leaf area, fresh and dry weight of shoot and root, total chlorophyll and leaf-N, P, and potassium (K) content, nitrate reductase, and carbonic anhydrase activity, total alkaloid content, seed-yield per plant, root- yield per plant and berries per plant. While 30 kg N ha^?1 proved best for root length, fresh and dry weights, as well as root-yield per plant. However, the effect of N and P fertilizers on carotenoid content, seeds per berry and 100-seed weight were insipid. Leaf- P remained unaffected by nitrogen application.     

A review on wood machining: characterization,optimization, and monitoring of the sawing process

ABSTRACT

Sawing is the most common machining process and is present in both primary and secondary wood transformation sectors. The objective of this paper is to review how it is affected by different factors. The current challenges associated with various machining factors, process monitoring, and sensor selection were identified and explained. Consequently, four research challenges and technological gaps were outlined. (1) Contradictory results have been reported due to different cutting processes, wood species, measurement methods, and cutting speed range studied in literature, so special consideration needs to be taken when comparing results with those available in the literature. (2) Laboratory test conditions cannot yet fully represent the extreme cutting conditions in sawmills. More emphasis should be placed on those harsh industrial cutting conditions. (3) Research on wood cutting lacks multi-objective optimization approach, which suggests that sawdust generation should be simultaneously analysed with surface quality and power consumption. (4) Compared with metal cutting, little research has been conducted on wood sawing monitoring using an artificial intelligence approach, which should be prioritized in designing adaptive control systems. Combining intelligent monitoring and multi-objective optimization approaches should pave the way for controlling the sawing process so higher surface quality and cost efficient machining is achieved.     

Performance of sheep offered ammonia, or urea-calcium hydroxide treated rice straw as an only feed

The effect of ammonia (AS) and urea‐calcium hydroxide (US) treated rice straw as a sole diet on the voluntary intake, digestibility of feed components, nitrogen retention, rumen parameters and microbial nitrogen yield of Suffolk ewes, were compared in a double 2 × 2 latin square design. The digestibility of feed components was not differed in sheep offered either AS or US. Dry matter intake was higher in AS (28 g/kg BW^0.75) than US (22 g/kg BW^0.75). Although there was high nitrogen intake in sheep given AS diet, this did not result in higher nitrogen retention compared with US diet. No difference was observed in purine derivatives or microbial nitrogen supply between the two groups. The rumen ammonia‐N of sheep offered AS or US exhibited a similar trend in diurnal change with the peak at 1.5–2.5 h after feeding and decline gradually thereafter. However, volatile fatty acid production peaked at approximately 8 h after evening feed and then steadily declined. AS and US diets led sheep to lose weight. In conclusion, urea‐calcium hydroxide treated straw can be used for sheep as an alternative to ammonia treated straw. Without supplementation, ammonia or urea‐calcium hydroxide treated straw was not sufficient even for the maintenance of sheep.