全文获取类型
收费全文 | 234篇 |
免费 | 13篇 |
专业分类
林业 | 25篇 |
农学 | 6篇 |
61篇 | |
综合类 | 9篇 |
农作物 | 20篇 |
水产渔业 | 21篇 |
畜牧兽医 | 94篇 |
园艺 | 4篇 |
植物保护 | 7篇 |
出版年
2023年 | 3篇 |
2022年 | 7篇 |
2021年 | 14篇 |
2020年 | 12篇 |
2019年 | 9篇 |
2018年 | 14篇 |
2017年 | 12篇 |
2016年 | 13篇 |
2015年 | 10篇 |
2014年 | 11篇 |
2013年 | 14篇 |
2012年 | 18篇 |
2011年 | 28篇 |
2010年 | 14篇 |
2009年 | 5篇 |
2008年 | 8篇 |
2007年 | 14篇 |
2006年 | 6篇 |
2005年 | 6篇 |
2004年 | 2篇 |
2003年 | 8篇 |
2002年 | 4篇 |
2001年 | 4篇 |
2000年 | 1篇 |
1999年 | 3篇 |
1998年 | 2篇 |
1993年 | 1篇 |
1987年 | 1篇 |
1984年 | 1篇 |
1980年 | 1篇 |
1974年 | 1篇 |
排序方式: 共有247条查询结果,搜索用时 15 毫秒
91.
92.
Salmon D Vanwalleghem G Morias Y Denoeud J Krumbholz C Lhommé F Bachmaier S Kador M Gossmann J Dias FB De Muylder G Uzureau P Magez S Moser M De Baetselier P Van Den Abbeele J Beschin A Boshart M Pays E 《Science (New York, N.Y.)》2012,337(6093):463-466
The parasite Trypanosoma brucei possesses a large family of transmembrane receptor-like adenylate cyclases. Activation of these enzymes requires the dimerization of the catalytic domain and typically occurs under stress. Using a dominant-negative strategy, we found that reducing adenylate cyclase activity by about 50% allowed trypanosome growth but reduced the parasite's ability to control the early innate immune defense of the host. Specifically, activation of trypanosome adenylate cyclase resulting from parasite phagocytosis by liver myeloid cells inhibited the synthesis of the trypanosome-controlling cytokine tumor necrosis factor-α through activation of protein kinase A in these cells. Thus, adenylate cyclase activity of lyzed trypanosomes favors early host colonization by live parasites. The role of adenylate cyclases at the host-parasite interface could explain the expansion and polymorphism of this gene family. 相似文献
93.
Noelia Zafra-Calvo Rowena Cerro Jorge M. Lobo Sahotra Sarkar 《Biological conservation》2010,143(3):787-794
Although the conversion of natural vegetation to agriculture threatens biodiversity, post-agricultural lands may provide an opportunity to preserve biodiversity if they are allowed to regenerate. We develop a framework for incorporating abandoned agricultural fields into the design of a Biosphere Reserve using former cocoa plantations on Bioko, Equatorial Guinea, as a case study. First, we used BIOCLIM to model the potential distribution of 62 ferns, 327 monocotyledons, 749 dicotyledons, seven primates, and 104 birds on Bioko. Next, we quantitatively assessed the representation of these distributions in conservation areas proposed by the Equatoguinean administration (hereafter “EPAs”). In addition, we used an area prioritization algorithm implemented in the ResNet software package to select an initial set of sites to serve as the Biosphere Reserve’s core areas, that is, intact forest in Bioko’s montane regions. Then, to augment the beta-diversity of the Reserve, we used the area prioritization algorithm to prioritize buffer zones in lowland sites including rainforest remnants and abandoned plantations that have partially regenerated to forest. We also compared the representation of biodiversity in the EPAs to its representation in Biosphere Reserves designed with ResNet. The representation of vegetation types and species in Reserves selected by ResNet that occupy 25% of the land on Bioko is equivalent to the representation achieved by the EPAs, which would cover 42% of Bioko. To conclude, we propose a conservation plan for Bioko. 相似文献
94.
Sclerotinia rot caused by Sclerotinia sclerotiorum is one of the most important diseases of processing tomatoes in Central Brazil. Yield losses in tomato cultivars (cv.) IPA-5 were assessed in mature plants from 1995 to 1997, and related to different disease intensities, in a naturally infested area irrigated by centre pivot. Over the 3 years, there were no differences (Tukey at 5%) in fruit numbers between plants without symptoms (NS) and with intermediate symptoms (IS), which yielded higher numbers than plants with severe symptoms (SS). The greatest reduction in fruit number was 56.8% in 1997. Significant differences were observed in fruit weight and size among NS, IS and SS plants in 1995 and 1997. In 1996, NS and IS plants were similar, but different from SS, which yielded significantly less. Weight and size reductions in SS plants reached 84.3% and 62.0%, respectively, in 1997. In 1996 and 1997, yield losses related to time of symptom appearance and physiological age were also assessed. Significant correlations were found ( P < 0.01), with nearly total losses observed when plants were infected from early to mid bloom, as opposed to plants infected near harvest, which had lower disease incidence and produced economically acceptable yields. Quadratic and exponential models best fitted the relationship between yield and time of symptom appearance, and yield vs physiological age could be explained by logistic and Gompertz functions. 相似文献
95.
Águeda González María del Mar Gil-Diaz María del Carmen Lobo 《Journal of Soils and Sediments》2017,17(5):1403-1412
Purpose
The objective of the study was to evaluate the tolerance of barley (Hordeum vulgare) and wheat (Triticum aestivum) in soil contaminated with different concentrations of Cd and Cr, the effect of these metals on some physiological characteristics and the biomass produced in order to assess their potential application in phytoremediation of contaminated soils.Materials and methods
A greenhouse experiment using two cultivars of barley, CB502 and Pedrezuela, and one of wheat, Albares, was conducted. The pots were watered with different concentrations of Cd or Cr during the period of plant growth. Cellular membrane damage, content and fluorescence of chlorophyll, and plant biomass were evaluated. After harvesting, the content of metals in the roots, shoots and grains was analysed as well as the available metal concentration in soil samples.Results and discussion
The results show that the Cd treatments did not significantly affect the growth and physiology of the plants, but they were affected by the Cr treatments. At the highest concentration of Cr, the decrease in chlorophyll content compared to the control was 44, 77 and 83 % for the CB502 and Pedrezuela barleys and the Albares wheat respectively. A reduction in biomass was also observed (74, 81 and 85 % respectively). The amount of Cd and Cr accumulated in the root was higher than that accumulated in the aerial part for the three cultivars. The barleys accumulated the highest amount of metal in the roots. The transfer factor was lower in barley than in the wheat for both metals.Conclusions
Cadmium treatments do not affect plant development. In the case of Cr, plant development and physiological traits were significantly affected. For the highest concentration of Cr, the CB502 barley had the highest tolerance index, 26 %, and the Albares wheat had the lowest one, 15 %. The two barleys were more effective in phytoremediation of soil contaminated with Cd or Cr than the wheat, presenting a higher tolerance to these metals in the assayed conditions.96.
Albin Lobo Erik Dahl Kjær Ditte Christina Olrik Lars-Göran Stener Jon Kehlet Hansen 《Annals of Forest Science》2018,75(2):62
Key message
Large genetic variation was found in Prunus avium L. populations from the northern parts of the species distribution range. The ranking of genotypes in terms of growth was stable when tested at three trial sites within the northern parts of the species distribution range.Context
Peripheral populations especially those in the leading edge are isolated from rest of the areas in the species distribution range. This can make them less genetically diverse yet genetically distinct from the rest of the populations in the species distribution range. Evaluation of their genetic diversity is thus crucial in understanding the local adaptation potential of a species.Aims
We investigated the genetic diversity and genotype by environment interaction at the northern parts of the distribution range of P. avium.Methods
Quantitative genetic variation of growth, stem form, and spring phenology were assessed in progenies from 93 plus trees of P. avium selected from 43 locations at the north of the species distribution range in Sweden and tested at two Swedish sites and one Danish site.Results
We find large quantitative genetic variation in growth and phenology at the northern part of the distribution range of P. avium. Only a limited genotype by environment interaction was observed with no clear indication of local adaptation at the northern parts of the species distribution.Conclusion
We conclude that P. avium harbors a high level of genetic diversity at the north of its distribution range. Present patterns therefore reflect more likely the recent introduction of the species and dispersal dynamics rather than a long-term loss of diversity along South-North ecological clines during the Holocene. With no indications of genetic depletion in growth or phenology, the gene pool in the breeding program is considered suitable for the future propagation of the species in the tested area.97.
The effectiveness of selecting umbrella species or groups of species to protect other groups is a key and unresolved issue in conservation planning. We detected areas important to the four groups of terrestrial vertebrates inhabiting Andalusia (southern Spain) according to four conservation-value criteria, i.e., richness, rarity, vulnerability, and endemicity. We calculated coincidences between criteria and taxa by the degree of correlation and fuzzy overlap between the different criteria for the same group (intra-group coincidence) and between the different groups of species for the same criteria (inter-group coincidence). For the groups to be appropriate umbrella species, inter-group coincidence should be high. The results showed that intra-group coincidence was high, but inter-group coincidence was low. Thus, zones of importance to each group of species tended to be located in different places compared to the other groups, and therefore none of them should be used as an indicator or umbrella group to protect the others. This result has implications for conservation policy, as the Andalusian authorities should not assume that protecting a site important to one group of species will also be relevant to conserve the other groups. Nevertheless, we identified the areas of relatively high conjoint importance for all the groups, and the best umbrella group in this region, which proved to be reptiles. 相似文献
98.
Aliny Simony Ribeiro José Ubirajara Vieira Moreira Pedro Henrique Braga Pierozzi Breno Francovig Rachid José Francisco Ferraz de Toledo Carlos Alberto Arrabal Arias Rafael Moreira Soares Claudia Vieira Godoy 《Euphytica》2007,157(1-2):15-25
The objective of this study was to investigate the genetic mechanisms of soybean resistance to Asian rust (Phakopsora pachyrhizi Syd. & P. Syd). F2 and F3 generations from 15 diallel crosses involving six soybean cultivars, FT-2, EMBRAPA 48, BRS 154,
BRS 184, BRS 214, BRS 231, were used to analyze the genetic control of Asian rust resistance in the soybean parents tested.
Genetic models were fitted to means and variances of the generations tested in a completely randomized field experiment with
5,700 hill plots. The experiment was spray-inoculated twice with an isolate that was first detected during the 2002/03 season
in Mato Grosso (MT) State and presently prevail in Central Brazil, at a six-day interval, on borders rows and on the useful
area, respectively, with a 104 spores/ml distilled H2O suspension. Assessments were made using a diagrammatic scale for disease severity at seven and 39 days after the first detection
of Asian rust in the experiments. Evaluations made in the second assessment (39 days) discriminated better between genotypes.
Selection at early plant developmental stages may not result in adult resistant plants. Cultivar FT-2, which had presented
monogenic resistance to a rust isolated that prevailed in the first two years of rust occurrence in Brazil, showed no resistance
to the MT State rust strain used in this experiment, but eleven crosses showed genetic variability for resistance in the second
assessment. Soybean rust resistant genes showing predominantly additive effects are dispersed among parents. Narrow sense
heritability values ranging from 0.42 to 0.74 at the F3 family level in the second assessment suggested that selection of
resistant genotypes is feasible. 相似文献
99.