Anti-diabetic potential of ursolic acid stearoyl glucoside: a new triterpenic gycosidic ester from Lantana camara

A new stearoyl glucoside of ursolic acid, urs-12-en-3β-ol-28-oic acid 3β-d-glucopyranosyl-4′-octadecanoate and other compounds were isolated from the leaves of Lantana camara L. The structure of this new glycoside was elucidated and established by standard spectroscopic methods. In streptozotocin-induced diabetic rats it showed significant reduction in blood glucose level.     

文冠果籽油生产生物柴油方法的研究进展

概述了利用碱催化法,固体酸催化法和生物酶法制备文冠果籽油生物柴油的研究进展,比较了3种制备方法的优劣:碱催化法是比较成熟的工业化方法,被广泛采用,具有反应快、成本低的特点,但污染大;固体酸催化法优点是反应快,温度低,无环境污染,催化剂和共溶剂还可循环利用;生物酶法对游离脂肪酸和水不敏感,醇用量小,无污染,但成本高、反应慢。提出了影响文冠果籽油生物柴油产业化发展的因素及对策。     

Sr胁迫对油菜幼苗抗氧化指标影响的研究

在石英砂和Hoagland营养液培养条件下,用浓度为0、10、20和40mmol/L的88Sr2+(88SrC l2)处理油菜幼苗(Brassica napus L.)(三叶期到六叶期),动态研究Sr胁迫对油菜幼苗抗氧化指标的影响,以及Sr2+积累量和富集能力的变化。结果表明,油菜幼苗对Sr2+表现出高富集能力,富集能力依次为:叶茎根,Sr2+浓度越高,富集能力越低。与对照相比,Sr2+胁迫下的油菜超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量随Sr2+浓度的增加及胁迫时间的延长呈先上升后下降趋势;过氧化物酶(POD)和过氧化氢酶(CAT)活性则随富集Sr2+能力的提高而降低。因此得出结论,油菜幼苗对Sr胁迫具有较高的耐受能力和富集能力;叶片是最主要的富集器官;富集能力与POD,CAT活性显著负相关。     

油茶人工高效杂交技术

为提高油茶杂交效率,大量创制新种质,以蕾期授粉为基础,通过对原有杂交技术的改进提出一套油茶人工高效杂交技术.基本步骤为:选处于松蕾阶段的母本花蕾,剪除全部花瓣和雄蕊,然后用父本的雄蕊直接对去雄后母本进行授粉,最后套袋标记.通过试验表明,该方法适用于种内不同品种间杂交组合的规模化配制,每杂交1朵花可获得杂交种子1.29～5.13粒;同时,还可以克服种间杂交的不亲和性,本试验获得了13粒宜春白皮中子与茶梨的杂交种子.     

Fine mapping of quantitative trait loci affecting organ weights by mouse intersubspecific subcongenic strain analysis

The genetic architecture of organ weights is not well understood. In this study, we fine‐mapped quantitative trait loci (QTLs) affecting organ weights by characterizing six intersubspecific subcongenic mouse strains with overlapping and non‐overlapping genomic regions on chromosome 2 derived from wild Mus musculus castaneus. QTLs for heart, lung, spleen and kidney weights were revealed on a 6.38‐Mb genomic region between two microsatellite markers, D2Mit323 and D2Mit472. Effects of the castaneus alleles at the organ weight QTLs were all opposite in direction to a body weight QTL previously mapped to the same genomic region. In addition, new QTLs for lung and kidney weights were revealed on a different 3.57‐Mb region between D2Mit205 and D2Mit182. Their effects were dependent on that of another body weight QTL previously mapped to that genomic region. The organ weight QTLs revealed were all duplicated in independent analyses with F₂ intercross populations between subcongenic strains carrying these QTLs and their background strain. The findings suggested that organ weights are not exclusively regulated by genetic loci that commonly influence overall body weight and rather that there are loci contributing to the growth of specific organs only.     

猪雄性生殖干细胞的分离培养及鉴定

本试验旨在探索猪雄性生殖干细胞(mGSCs)体外分离、培养的适宜条件,建立猪雄性生殖干细胞体外培养体系。采用两步酶消化法对新生小猪睾丸生殖干细胞进行了体外分离和初步的培养鉴定,并利用层黏连蛋白和明胶的不同贴壁特性,比较2种差易贴壁分选方法的富集效果,并对传代后的干细胞培养1周后进行碱性磷酸酶染色鉴定,通过免疫荧光技术检测培养细胞是否表达干细胞标志蛋白OCT-4。试验结果表明,层黏连蛋白更适用于猪生殖干细胞的富集、培养,细胞分选效率及增殖生长明显优于采用明胶分选的方法。培养的mGSCs拥有与小鼠mGSCs相同的形态、增殖及表达特征。鉴定结果显示,生长细胞克隆碱性磷酸酶染色呈阳性,支持细胞碱性磷酸酶染色呈阴性;培养的生殖干细胞克隆表达转录蛋白OCT-4,而饲养层支持细胞OCT-4抗体染色则呈阴性。结果表明培养的干细胞克隆仍保持较好的干细胞活性,保持正常的自我复制和分化潜能,初步建立了生殖干细胞培养体系。     

沙冬青种子萌发极限温度的研究

沙冬青抗旱能力强,对水分亏缺、极端高温、严寒低温、严寒霜冻、风蚀沙埋有特殊忍耐力,对干旱、半干旱的西部地区发展具有重要的生态、社会和经济意义,是目前利用和改造荒漠的先锋优良灌木。本文主要对沙冬青种子萌发的极限温度进行了研究,结果表明:理论上,沙冬青种子萌发的极限高温是42℃,发芽率为0;极限低温是6℃,发芽率为1.67%;最适温度为25℃,发芽率为98.00%。     

城市道路绿地植物配置研究

城市道路系统是城市的框架,城市道路绿地是城市绿化的廊道,利用园林树木的不同配置形式以线状或网状结构辐射全城,把城市各类园林绿地串联起来,组成完整的城市绿色生态网络,从而获得显著的生态效益,促进城市生态文明建设。城市道路绿地不仅可以起到调节城市温度、湿度,降低风速,改善城市小气候等多种生态功能,还可以起到美化城市,提升城市品位,方便交通等多方面的作用。