Chitin and glucans detected in the cell walls of Pythium dimorphum—an oomycetous fungus

With aid of fluorescein isothiocyanate (FITC) and gold-labelled lectins, together with antibodies against chitinase and β-1,3-glucanases, the spatial distribution of chitin and glucans in hyphal walls of Pythium dimorphum, a causative agent of root die-back disease of conifer seedlings was studied. Contrary to previous reports, this study revealed that chitin, a polymer of N-acetyl-d -glucosamine, is a component of the cell wall of this oomycetous fungus. However, compared with the other amino sugar, N-acetyl-galactosamine, it is not present in significant amounts. Ultrastructural and labelling studies demonstrated that the chitin region is embedded in or masked by glucan residues.     

Das Problem der Verwendung chemischer Schädlingsbekämpfungsmittel

Zusammenfassung Die Technisierung der Landwirtschaft und der Einsatz immer neuer chemischer Mittel in immer größerem Umfange zur Steigerung der Erträgnisse und Bewahrung der Ernte vor Verlusten sind, so bedauerlich dies auch aus vielen Gründen sein mag, nicht aufzuhalten.Über die Verantwortung und Verpflichtung aller Beteiligten, alles für den Schutz der mit der Anwendung der chemischen Schädlingsbekämpfungsmittel beschäftigten Menschen und der Masse der Konsumenten zu tun, kann kein Zweifel bestehen.Notwendig ist eine Umstellung im Denken vieler in der Praxis Tätigen, vor allem im Sinne einer Anerkennung der Eigenverantwortung für die eigene Gesundheit und die des Mitmenschen.Die Bearbeitung der vordringlichen Aufgaben und die Lösung der wichtigsten Fragen steht und fällt mit der Bereitstellung größerer Mittel für eigens zu errichtende Untersuchungsstellen, wobei die amerikanischen Verhältnisse in gewissem Umfange als Muster dienen können. Die Dringlichkeit und der Umfang der Probleme lassen eine oberflächliche und zweitrangige Bearbeitung unzweckmäßig erscheinen. Auf dem Gebiet der Gesunderhaltung der Bevölkerung gibt es wohl kaum eine wichtigere Aufgabe als die Bearbeitung der hier umrissenen Fragen.     

Tree crop based agroforestry in Nigeria: a checklist of crops intercropped with cocoa

A survey of cocoa farmers in Bendel, Ogun, Ondo and Oyo States of Nigeria, carried out in 1985, showed that almost all the farmers intercropped other crops with cocoa. The intercrops included food crops such as plantain (92.3), cocoyam (85.7%), cassava (51.3%), yam (41.3%), maize (38.9%), melon (31.4%), cowpea (28.6%) and pineapple (26.0%) and tree crops such as oil palm (71.5%), kola (67.3%), coffee (41.0%), coconut (7.9%) and citrus (7.2%). Other crops are ewe-iran (Sarcophrynium brachystachys) and ewe-gbodogi (Megaphrynium macrostachyum) (45.2%), african walnut (Tetracarpidium conophorum) (42.2%), aligator pepper (Aframomum melegueta) (31.6%), and iyere (Piper guinense) (20.2%). Guava, mango, pawpaw and vegetables such as celosia, okra and solanum occur in cocoa plots at rather low frequencies. As many as six or more other crops can be intercropped with cocoa at the same time.     

Changes in rates of photosynthesis and respiration during needle development of loblolly pine

Net photosynthetic rates of developing foliage and one-year-old foliage of loblolly pine (Pinus taeda L.) were measured under field conditions. In the subsequent year, net photosynthesis and dark respiration rates of current-year and one-year-old foliage were measured under controlled environmental conditions. Loblolly pine foliage grows slowly, reaching its final size 3.5 to 4 months after bud burst. Positive rates of net photosynthesis were recorded when the foliage was 13 and 18% of final length, in the controlled-environment and field study, respectively. However, because of high rates of dark respiration during the initial growth period, a positive diurnal carbon balance did not occur until foliage was about a third of final length (40 days after bud burst). Two months after bud burst, when foliage was about 55% of final length, its photosynthetic capacity exceeded that of one-year-old foliage. The highest rates of net photosynthesis were achieved when foliage was more than 90% fully expanded.     

Uleine and demethoxyaspidospermine from the bark of Plumeria lancifolia

The isolation of (+)-uleine (1) and (+)-demethoxyaspidospermine (2) from the bark of Plumeria lancifolia is reported along with (1)H- and (13)C-NMR data.     

Prevalence of thermophilic Campylobacter species in Swedish dogs and characterization of C. jejuni isolates

Background

The aims of this study were to investigate the prevalence of Campylobacter species in Swedish dogs, to identify the species of the Campylobacter isolates and to genotype the C. jejuni isolates. Young and healthy dogs were targeted and the sampling was performed at 11 veterinary clinics throughout Sweden from October 2011 to October 2012. Faecal swab samples were collected and sent to the laboratory at the National Veterinary Institute (SVA) for isolation of Campylobacter, speciation and genotyping.

Results

Campylobacter spp. were isolated from 67 of the 180 sampled dogs which yields an overall prevalence of 37%. The most prevalent species of Campylobacter among the participating dogs was C. upsaliensis with 52 of the 67 identified isolates. A lower prevalence was observed for C. jejuni with seven identified isolates and one isolate was identified as C. helveticus. Multi-locus sequence typing (MLST) was carried out on the seven C. jejuni isolates and all sequence types that were found are also commonly found in humans. The dogs were divided into three age groups; 1) under 12 months, 2) 12 to 23 months and 3) 24 months and older. The highest prevalence was found in the two younger age groups. Dogs shedding C. jejuni were between 3–12 months of age while dogs shedding C. upsaliensis were found in all ages.

Conclusions

The present investigation finds that Campylobacter spp. known to cause campylobacteriosis in humans are present in Swedish dogs. The results suggest an age predisposition where dogs under 2 years of age are more likely to shed Campylobacter spp. than older dogs. The most commonly isolated species was C. upsaliensis followed by C. jejuni, which was only detected in dogs up to 12 months of age. All C. jejuni isolates identified in the present study were of the same MLST types that have previously been described both in humans and in animals. The awareness of the Campylobacter risk of healthy young dogs may be an important way to reduce the transmission from dogs to infants, young children and immunocompromised adults.     

Daily Rhythm of Lactate Dehydrogenase in Rat (Rattus norvegicus) Carrying a Per1-luciferase Transgene: Assessment on Serum and Liver

Veterinary Research Communications -     

A Practical Technique for Electron Microscopy of Buffy Coats in Dogs and Cats

Plastic hematocrit tubes (PHTs) are convenient tools for electron microscopy (EM) of peripheral blood buffy coats, and the PHT‐EM technique is expected to be a practical method for veterinary clinical medicine. In this study, fixatives composed of various concentrations of sucrose, glutaraldehyde, and phosphate buffer (PB) were tested for preparing canine and feline buffy coats. The highest quality images were obtained using a fixative consisting of 2.5% glutaraldehyde in 0.1 m PB, and it was concluded that this method allows clinicians who are inexperienced in histological techniques can conveniently transport buffy coat samples to diagnostic laboratories for analysis by EM.