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91.
DeRossi R Silva-Neto AB Pompermeyer CT Frazílio FO Jardim PH de Barros AC 《Research in veterinary science》2012,92(2):278-282
The aim of this study was to evaluate 3 doses of levobupivacaine (LB) epidurally administered in sheep. Six adult male 24-36 month-old sheep received levobupivacaine at 3 doses, LB05 (0.05 mg/kg), LB15 (0.15 mg/kg), and LB25 (0.25mg/kg), and saline solution into the lumbosacral epidural space. Heart rate, arterial blood pressure (systolic, diastolic, and mean), respiratory rate, rectal, and skin temperature, local anesthesia, and ataxia were determined before treatment and at predetermined intervals. The duration of local anesthesia was 30±5 min, 145±27 min, and 290±18 min for LB05, LB15, and LB25, respectively (P<0.05). Ataxia determined for LB05, LB15, or LB25 was similar to the anesthetic times. There was an increase in heart rate and reduction in arterial pressure in LB25 (P<0.05), whereas LB05 or LB15 did not affect these parameters. Lumbosacral epidural levobupivacaine is an appropriate choice for local anesthesia in sheep. 相似文献
92.
Thais Borges Lessa Rafael Cardoso Carvalho André Luis Rezende Franciolli Lilian Jesus de Oliveira RodrigoSilvadaNunes Barreto David Feder Fabiana Fernandes Bressan Maria Angélica Miglino Carlos Eduardo Ambrósio 《Acta veterinaria Scandinavica》2012,54(1):73
Background
The diaphragm is the major respiratory muscle affected by Duchenne muscular dystrophy (DMD) and is responsible for causing 80% of deaths. The use of mechanical forces that act on the body or intermittent pressure on the airways improves the quality of life of patients but does not prevent the progression of respiratory failure. Thus, diseases that require tissue repair, such as DMD, represent a group of pathologies that have great potential for cell therapy. The application of stem cells directly into the diaphragm instead of systemic application can reduce cell migration to other affected areas and increase the chances of muscle reorganisation. The mdx mouse is a suitable animal model for this research because its diaphragmatic phenotype is similar to human DMD. Therefore, the aim of this study was to assess the potential cell implantation in the diaphragm muscle after the xenotransplantation of stem cells.Methods
A total of 9 mice, including 3 control BALB/Cmice, 3 5-month-old mdx mice without stem cell injections and 3 mdx mice injected with stem cells, were used. The animals injected with stem cells underwent laparoscopy so that stem cells from GFP-labelled rabbit olfactory epithelium could be locally injected into the diaphragm muscle. After 8 days, all animals were euthanised, and the diaphragm muscle was dissected and subjected to histological and immunohistochemical analyses.Results
Both the fresh diaphragm tissue and immunohistochemical analyses showed immunopositive GFP labelling of some of the cells and immunonegativity of myoblast bundles. In the histological analysis, we observed a reduction in the inflammatory infiltrate as well as the presence of a few peripheral nuclei and myoblast bundles.Conclusion
We were able to implant stem cells into the diaphragm via local injection, which promoted moderate muscle reorganisation. The presence of myoblast bundles cannot be attributed to stem cell incorporation because there was no immunopositive labelling in this structure. It is believed that the formation of the bundles may have been stimulated by cellular signalling mechanisms that have not yet been elucidated. 相似文献93.
Background
Intake of colostrum after birth is essential to stimulate intestinal growth and function, and to provide systemic immunological protection via absorption of Immunoglobulin G (IgG). The birth order and weight of 745 piglets (from 75 litters) were recorded during a one-week period of farrowing. Only pigs weighing greater than 0.68 kg birth weight were chosen for the trial. Sow colostrum was collected during parturition, and piglets were bled between 48 and 72 hours post-birth. Piglet serum IgG and colostral IgG concentrations were determined by radial immunodiffusion.Results
Sow parity had a significant (P < 0.001) effect on sow colostral IgG concentration, being 5% higher in multiparous females. Sow colostral IgG concentration explained 6% and piglet birth order accounted for another 4% of the variation observed in piglet serum IgG concentration (P < 0.05); however, birth weight had no detectable effect. Piglet serum IgG concentration had both a linear (P < 0.05) and quadratic effect (P < 0.05) on % survival. Piglets with 1,000 mg/dl serum IgG or less (n=24) had a 67% survival; whereas, piglets with IgG concentrations between 2250 to 2500 mg/dl (n=247) had a 91% survival. Birth order had no detectable effect on survival, but birth weight had a positive linear effect (P < 0.05). Piglets weighing 0.9 kg (n = 107) at birth had a 68% survival rate, and those weighing 1.6 kg (n = 158) had an 89% survival.Conclusion
We found that the combination of sow colostrum IgG concentration and birth order can account for 10% of the variation of piglet serum IgG concentration and that piglets with less than 1,000 mg/dl IgG serum concentration and weight of 0.9 kg at birth had low survival rate when compared to their larger siblings. The effective management of colostrum uptake in neonatal piglets in the first 24 hrs post-birth may potentially improve survival from birth to weaning. 相似文献94.
Fernando Santos Martín Manuel Bertomeu Meine van Noordwijk Rafael Navarro 《Small-Scale Forestry》2012,11(1):47-60
The ‘when, where and how’ of decisions by smallholder upland farmers to plant trees as part of their use of natural, human
and capital resources needs to be understood if policy support is to result in actual recovery of tree cover as part of a
‘forest transition’ trajectory. In large parts of the Philippines the turning point may be close. Data on resource access
and tree planting decisions were gathered from a household survey, with a total of 148 respondents in four rural communities
in Leyte Province in Central Philippines. Data were analysed using logistic regression analysis. Household-level results reveal
that the outcomes of the decision-making processes primarily depend on the availability of land and access to remaining forest
resources rather than socio-cultural or economic factors. The total area of land and number of parcels managed by the household
plus security of land tenure through ownership was found to have a statistically significant effect on farmers’ decision to
plant native timber trees. Access to surrounding natural forest is negatively associated with farmer tree planting. 相似文献
95.
96.
97.
Context
Pinus pinaster Ait. is found in the Iberian Peninsula under Mediterranean and Atlantic conditions. Both climates encounter each other in Galicia (NW Spain), where two bioclimatic regions can be differentiated: coastal and inland. A breeding program was launched in the coastal area, with two breeding and deployment areas delimited.Aims
We analyse plasticity patterns across regions in a coastal breeding population to assess the suitability of current breeding areas and how genetic material will likely respond to future climate.Methods
Total height at ages 3 and 8?years was assessed in 16 trials established along the coast and in inner Galicia. Clustering of environments with similar genotypic performance, family sensitivities to climatic factors and stability analyses were performed.Results
Sizeable genetic variation in plasticity was found among families, and crossover genotype-by-environment interactions were detected within and between regions. It was unfeasible to regionalize Galicia into alternative areas of stable genotypic performance. Only the cold regime was found to noticeably underlie the array of phenotypic responses to changing environmental conditions.Conclusions
Results suggest that previous delimitation in two breeding areas is pointless and indicate reduced effects of a changing climate towards Mediterranean conditions on decreasing population fitness. 相似文献98.
Yarira Ortiz-Alvarado Rafael Fernndez-Casas Carlos A Ortiz-Alvarado Emily Diaz-Iglesias Bert Rivera-Marchand 《Journal of insect science (Online)》2021,21(4)
Worker division of labor is a defining trait in social insects. Many species are characterized by having behavioral flexibility where workers perform non-typical tasks for their age depending on the colony’s needs. Worker division of labor and behavioral flexibility were examined in the little fire ant Wasmannia auropunctata (Roger, 1863), for which age-related division of labor has been found. Young workers perform nursing duties which include tending of brood and queens, and colony defense, while older workers forage. When nurses were experimentally removed from the colony, foragers were observed carrying out nursing and colony defense duties, yet when foragers were removed nurses did not forage precociously. We also administered juvenile hormone analog, methoprene, to workers. When methoprene was applied, foragers increased their nursing and defense activities while nurses became mainly idle. The behavioral flexibility of foragers of the little fire ant may be evidence of an expansion of worker’s repertoires as they age; older workers can perform tasks they have already done in their life while young individuals are not capable of performing tasks ahead of time. This may be an important adaptation associated with the success of this ant as an invasive species. 相似文献
99.
Mark Nelson Sally Silverstone Kelly C. Reiss Patricia Burrowes Rafael Joglar Molly Robertson 《Journal of Sustainable Forestry》2013,32(5):503-516
The impact on tree and amphibian diversity of line-planting of tropical hardwoods—mahogany (Swietenia macrophylla × S. mahagoni) and mahoe (Hibiscus elatus)—was studied in a secondary subtropical wet forest of Puerto Rico. Common coqui (Eleutherodactylus coqui) and melodious coqui (E. wightmanae) are the most frequent frog species; forest coqui (E. portoricensis) is less abundant. Although relative abundance means were slightly greater in the undisturbed forest and during the wet season, differences were not statistically significant suggesting that line-planting did not significantly affect amphibian diversity. The line-planted areas had a slightly higher, but not statistically significant diversity, richness, and evenness of tree species than the unplanted forest. Multi-response permutation procedure (MRPP) showed statistically significant community composition differences between line-planting and control plot trees (T = ?5.89, A = .86; p < .001). But mean similarity among plots in both the line-planted and control plots was relatively low at less than 50% of shared species, indicating high diversity of vegetation in the overall forest area. Canopy cover by tree species greater than 3 cm in dbh was much higher in the undisturbed forest but this difference may be reduced as the young line-planted hardwoods mature. Forest enrichment through line-planting of valuable timber species in secondary subtropical wet forest does not significantly affect tree diversity. 相似文献
100.
Daniel Moreno-Fernández Isabel Cañellas Rafael Calama Javier Gordo Mariola Sánchez-González 《Annals of Forest Science》2013,70(8):761-768