Immunohistochemistry Diagnosis of an Ovarian Dysgerminoma in One Bitch

An ovarian enlargement (diameter, 8 cm) was identified and surgically excised from a 5-year-old female dog. Microscopic examination of the multinodular neoplasm revealed sheets of polygonal neoplastic cells with large nuclei, frequent mitosis, necrosis and haemorrhage. Immunohistochemically, the neoplastic cells were positive for vimentin and alkaline phosphatase but were negative for CD3, CD79a, cytokeratin, alpha-fetoprotein, inhibin-α and S-100. The histopathological diagnosis of the mass was unilateral ovarian dysgerminoma.     

Ascorbic acid requirements in relation to wound healing in the cultured tilapia Oreochromis niloticus (Trewavas)

Abstract. Ascorbic acid depleted Oreochromis niloticus fingerlings were fed on each of three diets providing nil, normal and luxus levels of the vitamin, after small surgical incisions had been made in the dursoluteral musculature. Fish from each group were sampled regularly over 16 days and histopathological evaluation of the lesion area carried out. The tissue ascorbate levels were measured in each group during the study. The results showed that the epithelial element of the healing process developed irrespective of the vitamin level but although fibroblast activity was marked in all three groups, coltagenization was very much slower in the deficient group, and in these the lesion was not mature, even at the termination of the experiment.     

Induction of clinical aspergillomycosis by feeding contaminated diet to tilapia, Oreochromis niloticus (L.)

Abstract. Oreochromis niloticus maintained at 17°C and 26°C were fed a pelleted diet contaminated with cultures of Aspergitlus flavus fungus. Affected fish stopped feeding within one week, and became inactive, dark and oedematous. Exophthalmia, often accompanied by corneal or humoral changes, was common and mortalities commenced after 4 weeks. Surviving fish were sacrificed after 5 weeks and the fungus was isolated from all organs, including—irregularly—the eye. The histopathology was predominantly that of a necrotizing septicaemia, with fungal hyphae present in liver, peritoneum, kidney, intestinal wall and, distinctively, in the orbit and eye of affected fish.     

Qualitative and quantitative identification of L-gulonolactone oxidase activity in some teleosts

Abstract. L-gulonolactone oxidase activity was assessed in kidney and liver tissues of 14 teleosts by use of a histochemical technique for qualitative identification of the presence of enzyme activity and a biochemical technique for quantification of activity of the enzyme. Nine tilapias, two cyprinids and three salmonids were investigated; enzyme activity was only detected in liver and kidney tissues of common carp, Cyprinus carpio L., and kidney tissue of Oreochromis spilurus (Günther) and O. aureus (Steindachner).     

Caudal anaesthesia of the infraorbital nerve for diagnosis of idiopathic headshaking and caudal compression of the infraorbital nerve for its treatment,in 58 horses

Reasons for performing study: Idiopathic headshaking is often a facial pain syndrome, but a diagnostic protocol has not been described. In a previous study, caudal compression of the infraorbital nerve for treatment offered a fair success rate, but low case numbers and short follow‐up time were limitations. Objectives: To describe a diagnostic protocol for headshaking, examining the role of bilateral local analgesia of the posterior ethmoidal nerve (PET block). To report longer‐term follow‐up after surgery of the original cases and further cases and to determine whether changes to the technique influence success rates and complications. Methods: Records of horses that had undergone PET block and caudal compression surgery at 3 hospitals were reviewed. Modifications to the surgical technique included placing additional coils into the infraorbital canal and/or performing concurrent laser cautery of the nerve. Follow‐up information was obtained by telephone contact with owners. Results: The PET block was performed in 27 horses, with a positive result in 23 of 27 (85%). Surgery was performed in 58 horses. A successful outcome was initially achieved in 35 of 57 (63%) horses, but recurrence occurred between 9 and 30 months later in 9 (26%). Surgery was repeated in 10 of 31 (32%) horses. Final success rate, considering only response to the last performed surgery, was 28 of 57 (49%) horses with median follow‐up time of 18 months (range 2–66 months). Nose‐rubbing was reported post operatively in 30 of 48 (63%) horses. This resolved in all but 4 horses, which were subjected to euthanasia. Response to PET block or change in surgical technique did not appear to influence outcome or complications. Conclusions and potential relevance: The diagnostic protocol described is recommended for the investigation of headshakers. Caudal compression offers the best prognosis for a successful outcome compared with other treatments, for horses in which the only alternative is euthanasia. Surgical treatment of the disorder requires refinement, and the pathogenesis of the disorder requires investigation.