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排序方式: 共有115条查询结果,搜索用时 437 毫秒
111.
JP MORRIS RM ONG JK O'DWYER JL BARNETT PH HEMSWORTH IJ CLARKE EC JONGMAN 《Australian veterinary journal》1997,75(12):883-886
Objective To examine associations between late event-related cerebral potential amplitudes and behavioural responses to noxious electrical stimulation as an indicator of acute pain in sheep.
Design Analysis of variance for the effects of stimulus intensity on the behaviour and event-related cerebral potential variables.
Procedure Ninety-six brief constant current electrical pulse trains were presented to the front left leg of eight sheep at four intensities (2.5 to 10 mA) in a randomised order. An event-related cerebral potential and a graded flinch response were recorded for each stimulus and the 24 event-related cerebral potentials at each intensity were averaged to produce a mean waveform. Various components of this waveform were analysed and changes in these measures and the sheep's flinch response, as stimulus intensity increased, were determined.
Results Both the flinch response and some event-related cerebral potential components, that is, peak amplitudes 114 {N1}, 187 {P1}, 318 {P2} and 230 {Pm} ms after stimulus onset, were significantly affected as stimulus intensity increased.
Conclusion These corresponding behavioural and event-related cerebral potential changes indicate the usefulness of using changes in the event-related cerebral potential to measure acute pain in sheep. 相似文献
Design Analysis of variance for the effects of stimulus intensity on the behaviour and event-related cerebral potential variables.
Procedure Ninety-six brief constant current electrical pulse trains were presented to the front left leg of eight sheep at four intensities (2.5 to 10 mA) in a randomised order. An event-related cerebral potential and a graded flinch response were recorded for each stimulus and the 24 event-related cerebral potentials at each intensity were averaged to produce a mean waveform. Various components of this waveform were analysed and changes in these measures and the sheep's flinch response, as stimulus intensity increased, were determined.
Results Both the flinch response and some event-related cerebral potential components, that is, peak amplitudes 114 {N1}, 187 {P1}, 318 {P2} and 230 {Pm} ms after stimulus onset, were significantly affected as stimulus intensity increased.
Conclusion These corresponding behavioural and event-related cerebral potential changes indicate the usefulness of using changes in the event-related cerebral potential to measure acute pain in sheep. 相似文献
112.
113.
Quantitative analysis of chondroitin sulfate isomers in intervertebral disk chondrocyte culture using capillary electrophoresis 总被引:1,自引:0,他引:1
Maeda S Miyabayashi T Yamamoto JK Roberts GD Lepine AJ Clemmons RM 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(9):1039-1043
Chondroitin sulfate (CS) isomers, 6-sulfate (CS6) and 4-sulfate (CS4), change their ratio to each other in cartilaginous tissues with aging. In this study, a quantitative measurement method of CS6 and CS4 was developed, using capillary electrophoresis (CE). Various buffer solutions, pH, and digestion times were studied, and the use of 0.1 M Tris-HCl at pH of 8.0 allowed the isolation of CS6 and CS4 from CS most efficiently when combined with chondrotinase ABC at a concentration of 1 mU/microg of the substrate during a 3 hr digestion period. Amounts of newly synthesized CS6 and CS4 in the intervertebral disk chondrocyte three-dimensional culture were quantified by this method after the proteoglycans were extracted by equilibrium density centrifugation. 相似文献
114.
Hiroaki Kamishina Jennifer A. Cheeseman Roger M. Clemmons 《Veterinary research communications》2009,33(7):645-657
The present in vitro study was designed to evaluate whether canine bone marrow stromal cells (BMSCs) promote neurite outgrowth from dorsal root
ganglion (DRG) neurons. Bone marrow aspirates were collected from iliac crests of three young adult dogs. DRG neurons were
cultured on BMSCs, fibroblasts, or laminin substrates. DRG neurons were also cultured in BMSC- or fibroblast-conditioned media.
DRG neurons grown on BMSCs extended longer neurites and developed a much more elaborate conformation of branching neurites
compared to those on fibroblasts or laminin. Quantitative analysis revealed that these effects were associated with the emergence
of increased numbers of primary and branching neurites. The effect appears to be dependent upon cell-cell interactions rather
than by elaboration of diffusible molecules. With more extensive investigations into the basic biology of canine BMSCs, their
ability for promoting neurite outgrowth may be translated into a novel therapeutic strategy for dogs with a variety of neurological
disorders. 相似文献
115.
LFC Brito JB Engiles RM Turner LM Getman A Ebling 《Reproduction in domestic animals》2009,44(5):846-851
An 18-year-old Friesian stallion was examined approximately one week after reportedly presenting scrotal swelling due to torsion of the spermatic cords. Upon presentation no scrotal swelling was noted, the testes were normally oriented, and no abnormalities of the spermatic cords were noted. However, both testes were smaller than expected for a mature stallion and deep palpation revealed that the consistency of the testes was nodular. Ultrasonographic evaluation of the testes revealed diffuse heterogeneous parenchyma with multiple hypoechoic nodular areas. Grossly, the testicular parenchyma was effaced by multiple gray-tan nodules of varying consistency interspersed with gray-white bands of tissue. Microscopic analysis revealed multiple pleomorphic neoplastic foci disseminated throughout both testes. Histological and immunohistochemical features were atypical and consistent with the diagnosis of bilateral testicular mixed germ cell-sex cord stromal tumours. Bilateral testicular tumours and testicular mixed cell tumours are extremely rare in stallions and this is the first report of bilateral testicular mixed germ cell-sex cord-stromal tumours in a stallion. Our findings indicate that certain ultrasonographic characteristics are suggestive of testicular tumour and that immunohistochemistry markers can be used to better characterize testicular neoplasms in stallions. 相似文献