六味地黄汤及其君药对小鼠免疫调节作用的影响

为探讨六味地黄汤和地黄对小鼠免疫功能的影响,分别给小鼠灌服高、低剂量的六味地黄汤复方和地黄水提液,测定小鼠的体重、胸腺和脾重量,腹腔巨噬细胞吞噬功能及血液中白细胞和红细胞数。结果表明,试验组小鼠的体重、胸腺和脾脏重量、血液中淋巴细胞数和红细胞数增加,腹腔巨噬细胞吞噬功能增强。提示高剂量的六味地黄汤复方和地黄均可增强小鼠机体的免疫功能,且六味地黄汤复方的效果优于地黄。     

徒手克隆工具自制及改进

徒手克隆技术(handmade somatic cell cloning,HMC)解决了由于昂贵设备对核移植技术应用的制约问题。通过徒手克隆刀的自制,不但进一步节省了成本,且更能符合使用者的习惯,提高总的切割效率。试验结果表明,利用自制克隆刀进行半卵法去核,可以达到每小时切割496枚卵母细胞的切割速度和88.14%的存活率;自制聚集针具有制作简单,可以打磨、效果好等特点,可以进一步简化和降低徒手克隆技术的成本,提高克隆效率。     

努力实现畜牧高科技产业化

伴随着新年钟声的敲响 ,我们迎来了新世纪的曙光。回首往事 ,我们心潮澎湃 ,展望未来 ,我们豪情满怀。作为刚刚被农业部、国家发展计划委员会、国家经济贸易委员会、财政部等八部委联合评定的农业产业化国家重点龙头企业 ,我们深感重任在肩。面对新的世纪 ,我们不能停留在单纯的饲料、兽药、生物制品的研制生产阶段。实现畜牧生物高新技术产业化 ,将是我们新的追求。新疆有着发展畜牧业得天独厚的资源优势和地域条件 ,而落后的生产技术和经营方式 ,却严重制约着新疆畜牧业的腾飞。在今后的几年里 ,天康公司将以畜牧生物技术研发为核心 ,以优…     

Fatal infection in human flora-associated piglets caused by the opportunistic pathogen Klebsiella pneumoniae from an apparently healthy human donor

Seventeen out of 24 human flora-associated (HFA) piglets died after oral administration of whole fecal flora from an apparently healthy human donor. The bacteria isolated from the organs of the infected piglets were identified as Klebsiella pneumoniae by bacteriological and biochemical tests and 16S rRNA gene sequence analysis. The identical K. pneumoniae strain was also isolated from the donor's fecal flora. All three neonatal piglets inoculated with K. pneumoniae from the donor's fecal flora developed severe diarrhea, with 2 eventually dying. This strongly suggests that the opportunistic pathogen K. pneumoniae from the human donor caused the fatal infection in the HFA piglets. The results underscore the importance of safety evaluation of the human donor's fecal flora for HFA piglet development.     

畜牧业对江河源区生态环境演变的影响机理研究

草地畜牧业是江河源区主要的生产方式,也是人类生产活动影响这一地区生态环境演变的主要方式,在系统回顾和总结江河源区畜牧业经济发展的历史过程的基础上,提出畜牧业对环境的作用主要取决于游牧空间、畜群数量、畜群结构、草地的自我恢复能力,建立江河源区草地畜牧业对其生态环境演变作用的机理模型,利用该模型对畜牧业发展过程中出现的游牧和固定放牧2种模式对区域生态环境的演变进行了定性分析.     

Major histocompatibility complex and mate choice in the polygynous primate: the Sichuan snub‐nosed monkey (Rhinopithecus roxellana)

The highly polymorphic genes within the major histocompatibility complex (MHC) not only play a major role in immunity resistance, but also seem to provide hints for mate choice in some animal populations. In the present study we investigated MHC‐related mate choice in a small natural population (group size 40–55 individuals) of a polygynous primate, the Sichuan snub‐nosed monkey (Rhinopithecus roxellana). We found that there was no evidence either for MHC‐disassortative mating, or for females to mate with males based on MHC heterozygosity or specific alleles. Nevertheless, of the 11 alleles identified, we found that the frequencies of 2 alleles, Rhro‐DRB2 (P < 0.01) and Rhro‐DRB5 (P < 0.05) were higher in offspring than in their parents. These findings suggest that MHC‐DRB in this population of R. roxellana is unlikely to be associated with mating preferences. Limited female opportunities for mate choice are likely due, in part, to the harem breeding structure present in R. roxellana, and the relatively small number of resident adult males in our study band (N = 4–6). In addition, we suggest that differences in the frequency of particular alleles across generations may be linked to parasite resistance in a fluctuating environment; however, confirmation of this finding requires further study.     

UHT乳在货架期内理化性质的变化研究

本文研究了UHT全脂乳在37℃温度下,酸度、黏度、蛋白水解度和酒精稳定性的变化情况,并得出UHT乳在37℃温度下的货架期。     

吐温-80对液氮冷冻处理的双歧杆菌的冻伤保护

向双歧杆菌培养基中添加吐温-80，可增加双歧杆菌的细胞膜柔韧性和弹性。经液氮冷冻处理24h后，测定双歧杆菌细胞漏出液中乳酸脱氢酶的活力，结果表明添加吐温-80，可显著降低细胞培养液中的乳酸脱氢酶的漏出率（p〈0．05），其中以添加0．025％吐温-80的效果尤为明显。     

动物卫生监督执法中自由裁量权影响因素及其对策探讨

前言行政自由裁量权是行政执法过程中大量存在的一种权力,如何规范行使自由裁量权是做到行政处罚公开、公正、责罚相当的关键。2011年8月4日,中共中央办公厅、国务院办公厅印发《关于深化政务公开加强政务服务的意见》进一步强调了"严格规范行政裁量权行使,细化、量化裁量基准,公开裁量范围、种类和幅度"。动物卫生监督执法体系涉及大量的自由裁量条款,在动物卫生监督执法中,如何分析把握影响因素、严格规范自由裁量权的行使,是当前摆在我们面前的一个重要课题。     

大豆活性成分在畜禽生产中的应用研究进展

主要介绍大豆活性成分及其在畜禽生产中的应用,旨在为研究者提供理论依据,为畜禽生产者提供指导。