Analyses of monoclonal antibodies reacting with porcine CD3: results from the Second International Swine CD Workshop

Among the 57 monoclonal antibodies (mAb) analyzed within the T-cell group from the Second Swine CD Workshop, six mAb fell within clusters T10 and T11 (No. 088, STH164; No. 148, FY1A3; No. 149, FY2C1; No. 150, FY1H2; No. 151, FY2A11; No. 169, BB23-8E6). The mAb within these two groups gave a similar appearance on flow cytometry and stained all peripheral blood T-cells as defined by CD4 and wCD8 staining. All six mAb precipitated a 24 kDa protein. On the basis of inhibition analyses performed as part of the workshop and from published data, the mAb define at least three epitopes. There is only minimal stimulation of resting peripheral lymphocytes, but four of the mAb produce strong stimulation in the presence of PMA. With the exception of STH164, all have been shown to react with CD3-transfected COS cells. The new mAb, therefore, react with three epitopes on porcine CD3 designated CD3a (BB23-8E6, FY2A11), CD3b (FY1A3, FY2C1), and CD3c (FY1H2). mAb STH164 appears to be reactive with another epitope, however, since its reactivity with CD3 has not been confirmed it is designated as wCD3.     

Water Quality in Laboratory Soil-Water Microcosms with Soils from Different Areas of Thailand

Forty-five soil samples were collected from aquaculture areas in 23 provinces of Thailand to include six soil orders and wide variation in physical and chemical properties. Soil-water microcosms were prepared containing 5 g of soil and 150 mL of distilled water. Microcosms were held on an oscillating table shaker (150 rpm) for 1 wk at 25 C in the dark. Water pH and concentrations of dissolved nutrients, total alkalinity, and total hardness were measured. Differences in properties within soil orders caused wide variation in composition of solutions and differences in concentrations of dissolved substances and pH were not related to soil order. Regression analyses revealed significant correlations between concentrations of soil nutrients extractable in dilute acid (0.05 N HCI plus O.025 N H₂SO₄) or in neutral, 1 N ammonium acetate and aqueous concentrations. Regression coefficients usually were higher for dilute-acid extractable nutrients than for ammonium acetate extractable ones. Regression coeficients based on dilute-acid extractable nutrients follow: soluble reactive phosphorus ( r = 0.816); calcium ( r = 0.685); magnesium ( r = 0.470); potassium ( r = 0.959); sodium ( r = 0.977); manganese ( r = 0.462); boron ( r = 0.399). The correlation between soil and solution iron was not significant and aqueous concentrations of copper and zinc were below detection limit. Hardness was correlated with soil carbon ( r = 0.710) and soil pH was a good predictor of alkalinity ( r = 0.877). Soil pH and aqueous pH were highly correlated (r = 0.939). Findings suggest that soil characteristics can be used to predict pH and concentrations of several dissolved substances in soil-water systems under aerobic conditions.     

Effect of exogenous glucagon and free fatty acids on gluconeogenesis in fasting neonatal pigs

Thirty-two pigs (1 d old) were used to determine if exogenous glucagon and(or) free fatty acids (FFA oleic acid) would enhance gluconeogenesis and glucose homeostasis during fasting. Pigs were acquired at birth, fitted with an indwelling arterial cannula (via umbilicus) and fasted 24 h to deplete liver glycogen. A jugular cannula was inserted nonsurgically 8 to 10 h before initiation of a primed-continuous infusion consisting of control (excipient), glucagon (Glu), oleic acid (FFA), or both glucagon and oleic acid (Glu-FFA). Plasma Glu averaged 395 pg/ml preinfusion and was similar across treatments. The concentration increased fivefold (P less than .05) by 80 min for Glu and Glu-FFA pigs and remained constant thereafter (160 min: 2,379, 2,258 pg/ml; 240 min: 2,355, 2,274 pg/ml, respectively). Glucagon infusion did not alter plasma glucose after 240 min of infusion (control, 50 vs Glu, 51 mg/dl); however, Glu-FFA effected an increase (60 mg/dl, P less than .10). In contrast, pigs infused with FFA alone had a lower glucose concentration (40 mg/dl, P less than .10). Rate of glucose synthesis was determined using liver slices, acquired immediately postinfusion, with alanine and lactate as substrate (7.5 mM). The rate of synthesis was not altered by Glu or Glu-FFA infusion (2.91, 2.43 mumol glucose X g-1 X h-1 vs 2.91 for control). In contrast, exogenous FFA reduced synthesis to 1.85 mumol glucose X g-1 X h-1 (P less than .05) with lactate as substrate. It appears that Glu is not the primary factor limiting gluconeogenesis in fasting newborn pigs.(ABSTRACT TRUNCATED AT 250 WORDS)     

母猪矿物质供应量应随年龄增长而增加

母猪妊娠期的饲喂应该随母猪年龄的增长而调整,否则母猪微量养分的实际摄入量和机体维持和繁殖对微量养分的需要量之间就会出现越来越大的差距。     

Rundschau

Ohne ZusammenfassungPflanzenschutzorganisation für Europa und die Mittelmeerländer     

Umweltorientierter Pflanzenschutz

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Use of middle infrared radiation to estimate the leaf area index of a boreal forest

The leaf area index (LAI) of boreal forest can be estimated using reflected radiation recorded by satellite sensors. Measurements of visible and near infrared radiation are commonly used in the normalized difference vegetation index (NDVI) to estimate LAI. However, research, mainly in tropical forest, has demonstrated that LAI is related more closely to radiation of middle infrared wavelengths than of visible wavelengths. This paper derives a vegetation index, VI3, based on radiation from vegetation recorded at near and middle infrared wavelengths. For a boreal forest canopy, the relationship between VI3 and LAI was observed to be much stronger than that between NDVI and LAI. In addition, the LAI estimated using VI3 accounted for about 76% of the variation in field estimates of LAI, compared with about 46% when using the NDVI. We conclude that information provided by middle infrared radiation should be considered when estimating the leaf area index of boreal forest.     

Mechanical site preparation effects on understory plant diversity in the Piedmont of the southern USA

Diversity of understory vegetation was compared among four intensities of site preparation and an adjacent 50-year-old pine-hardwood forest. The study site was a six-year-old loblolly pine (Pines taeda) plantation in the lower Piedmont of Georgia. Species richness and Shannon diversity indices were used to evaluate vine and woody (trees and shrubs) species diversity. Biomass distribution was compared among four plant categories: vines, forbs, grasses, and woody. Moderate intensity treatments (chainsaw and shear and chop) consistently ranked highest overall in diversity, with the mature pine-hardwood forest ranking lowest. Distribution of the four plant categories was not significantly affected by intensity of site preparation.Alabama Agricultural Experiment Station Series No. 9-892325P.     

Association of key foods and beverages with obesity in Australian schoolchildren

OBJECTIVE: To examine the pattern of intake of key foods and beverages of children aged 4-12 years and the association with weight status. DESIGN AND SETTING: A computer-assisted telephone interview was used to determine the intake of fruit, vegetables, packaged snacks, fast foods and sweetened drinks 'yesterday' and 'usually' as reported by parents/guardians of a representative sample of 2184 children from the Barwon South-Western region of Victoria, Australia. RESULTS: Children who consumed >2-3, >3-4 and >4 servings of fruit juice/drinks 'yesterday' were, respectively, 1.7 (95% confidence interval (CI) 1.2-2.2), 1.7 (95% CI 1.2-2.5) and 2.1 (95% CI 1.5-2.9) times more likely to be overweight/obese compared with those who had no servings of fruit juice/drink 'yesterday', adjusted for age, gender and socio-economic status (SES). Further, children who had > or = 3 servings of soft drink 'yesterday' were 2.2 (95% CI 1.3-3.9) times more likely to be overweight/obese compared with those who had no servings of soft drink 'yesterday', adjusted for age, gender and SES. In addition, children who 'usually' drank fruit juice/drinks twice or more per day were 1.7 (95% CI 1.2-2.4) times more likely to be overweight/obese compared with those who drank these beverages once or less per week, adjusted for age, gender and SES. Although fast foods and packaged snacks were regularly eaten, there were no associations between weight status and consumption of these foods. CONCLUSIONS: Intake of sweetened beverages was associated with overweight and obesity in this population of Australian schoolchildren and should be a target for intervention programmes aimed at preventing unhealthy weight gain in children.