An equine cryptorchid with testicular and ovarian tissues.

Cytogenetic and histological studies were carried out on an intersex horse which was diagnosed clinically as a cryptorchid. Surgery confirmed the horse to be a bilateral abdominal cryptorchid and histological examination revealed ovarian tissue associated with the left epididymis. Chromosome analysis of cultured cells from testicular tissue, ovarian tissue and skin revealed 64-XX and 64-XY make-up, the left gonad containing a greater preponderance of XX cells over XY cells. The external characteristics and behaviour of the horse were indistinguishable from that of a "routine" cryptorchid. Other cases of equine intersexes are reviewed and theories for the discrepancies between genetic, gonadal and phenotypic sex are discussed.     

The effect of soil aggregate size and water content on herbicide concentration in soil water

Suspensions of wettable powders of metribuzin and simazine were sprayed onto samples of two soils of two particle size grades, > 2.5 mm and <0.5 mm. The soils were either air-dry or at a water content of 12%. After either 1 h or 1 week, water was added to give a soil to water ratio of 1:1. Samples of solution were analysed after 1, 24 and 48 h. With metribuzin the intial concentration for both soils after wetting was greater from the >2.5 mm samples than the <0.5 mm samples, following application to dry soil, but by 48 h the differences were negligible. With simazine WRO soil did not show this effect at all and with Kirton soil only small differences were seen. If the soil was wet at the time of application, particle size had no effect except with simazine in Kirton soil. Generally metribuzin concentrations were higher after application to wet than to dry soils for at least 24 h after wetting whereas simazine concentrations were higher from initially dry soils and the differences had virtually disappeared 24 h after wetting. With the WRO soil herbicide concentrations in soil water were higher if the soil was wetted 1 h after spraying than if left for 1 week but the differences rarely persisted for 48 h. No such trend was observed with Kirton soil. It is concluded that the differences observed in these experiments could be responsible for variations in the performance of soil-applied herbicides.     

The selectivity of methabenzthiazuron between barley, perennial ryegrass and Poa trivialis

In field experiments methabenzthiazuron at 1.1 kg ai/ha applied pre-emergence gave good control of Poa trivialis L. in perennial ryegrass S23 which was undersown in spring barley (Zephyr); no significant damage to barley or perennial ryegrass resulted from 2.2 kg ai/ha. However this dose applied when barley had three to four leaves decreased the subsequent barley yield. In pot experiments in the glasshouse methabenzthiazuron was active mainly due to uptake through the soil. Much greater damage to barley and perennial ryegrass resulted when herbicide-treated soil was placed below seed level than above the seed. The difference was less marked with P. trivialis. The latter started to develop secondary roots near the soil surface earlier than either perennial ryegrass or spring barley.     

Structure-activity relationships of 1,2,3-benzothiadiazoles as synergists for carbaryl against the house fly (Musca domestica)

Fifty six 1,2,3-benzothiadiazoles and related compounds were evaluated as carbaryl synergists against the house fly (Musca domestica). Many of these were excellent synergists, the most active being those containing various combinations of halogen, alkyl, or alkoxy substituents in the 5- and/or 6-positions of the ring.Regression analysis on the data from 14 compounds for which substituents constants were available established that synergistic activity can be satisfactorily described by equations in terms of the hydrophobic bonding constant (π) and the homolytic free radical constant (σ ·).The results with compounds related to the 1,2,3-benzothiadiazoles suggest that synergistic activity is associated primarily with the diazosulfide moiety.     

Variability of hard parts of opisthaptor of two species of Gyrodactylus nordmann, 1832 (Monogenoidea) from Phoxinus Phoxinus (L.).

The morphological and metrical variability of hard parts of opisthaptor was studied in the representatives of local populations of Gyrodactylus aphyae Malmberg, 1957 and G. macronychus Malmberg, 1957. The changes within the range of variability, which were observed during the year, correspond to regular and periodical changes in the environmental temperatures. The hard parts of opisthaptor were much smaller in specimens occurring during warm seasons than in specimens occurring during cold seasons. The temperature of about 8 degrees C forms a boundary between warm and cold seasons. The author assumes that similarly as G. aphyae and G. macronychus also other members of the genus Gyrodactylus living in climatic zones with regular change of seasons will react similarly on the annual variation in the environmental temperatures.     

The "barker" (neonatal respiratory distress) syndrome in the pig: its occurrence in the field.

A new syndrome, characterised by acute respiratory distress and by abnormalities of the skin, hair and thyroid, was the apparent cause of neonatal death in 35 (24 per cent) of 146 piglets sired by a large white boar in four small commercial pig units. The syndrome appears to be of genetic origin, inherited as an autosomal recessive trait. Its similarities with the respiratory distress syndrome (RDS) of the newborn infant and the "barker" syndrome of the newborn foal are discussed.     

Comparative enzyme activities and cytochrome P-450 levels of some rat tissues with respect to their metabolism of several pesticides

Cytochrome P-450, A- and B-esterase, amidase, and glutathione S-aryl transferase were assayed in the postmitochondrial centrifugal fraction, microsomes, and supernatant of rat liver, lungs, kidneys, and testes. Liver microsomes contained the highest P-450 levels and A-esterase activity. B-esterase activity was more generally distributed and higher in the microsomal tissue fractions. Microsomal amidase activity was highest in rat lung and lowest in the liver (per mg protein). Glutathione S-aryl transferase activity was highest in the liver. The in vitro metabolism of carbaryl, phosphamidon, and chlorotoluron by the various centrifugal fractions revealed many differences. Carbaryl metabolism was greater in the liver microsomal fractions than in any other preparation. 1-Naphthol was the major metabolite in all tissue fractions. Although very little metabolism of phosphamidon occurred in the rat, metabolism in the rat liver postmitochondrial fraction was slightly higher with respect to the production of metabolites than in the supernatant and microsomes combined. Chlorotoluron was not metabolized by any of the tissue fractions of the rat. At least a low level of activity toward some compounds was observed in all tissues, but this study confirmed that the liver was the most active metabolizing tissue as well as having the highest levels of enzymatic activity usually associated with pesticide metabolism.