MD病毒抗独特型抗体的研制

制备鸡马立克氏病病毒(MDV)抗独特型抗体(Ab2),探讨Ab2模拟抗原诱导免疫应答的能力.用MDV免疫SPF鸡,分离提纯鸡抗MD-IgG(Ab1),Ab1免疫BALB/C鼠,用鼠脾细胞与SP2/0细胞制备Ab2杂交瘤细胞株,间接ELISA筛选阳性杂交瘤细胞株,经克隆、纯化2×株抗MDV独特型抗体杂交瘤细胞株(6E5,7A12),其中6E5培养上清、腹水ELISA效价分别为4000×、10000×,免疫20 d攻毒,具有抗MDV感染保护.     

~(60)Co-γ射线对玉米自交系的诱变效应

用3种不同剂量60Co-γ射线辐射处理6个玉米自交系种子,研究对玉米自交系M1主要性状的诱变效应,结果表明:①主要株型性状都表现出不同程度的抑制作用,而主要雄穗性状和果穗性状却既有促进作用也有抑制作用;②处理后群体内各性状的变幅和变异系数均大于对照,表明60Co-γ射线能创造丰富的遗传变异。同时结合不同性状的诱变效应,探讨了在实践中对研究材料适宜的60Co-γ射线辐射剂量。     

鱼类早期发育阶段摄食行为研究现状

对鱼类早期阶段的摄食行为研究进行了综述。重点阐述了胃含物分析法、饥饿对摄食的影响、摄食器官的形态发育特征与摄食行为的关系、食物选择及其生理生态因子对摄食的作用。初步探讨了仔稚鱼饥饿后的捕食量和捕食速度的变化,并结合食物保障,分析了食物选择性指数I在鱼类摄食行为研究中的应用。     

数字化地籍测量中定向错误的类型与改正方法

数字化地籍测量中,利用控制点进行定向操作时容易出现错误。如果定向出现问题,将导致该站所测的界址点均出现错误。本文根据不同的错误类型,推导出了不同的数学模型。有些定向错误可以通过数学模型修正,有的错误类型则无法修正,当尽量避免。     

牛胚胎移植技术的产业化研究

本文探讨了引进国外优质奶、肉牛冷冻胚胎建立核心群，以及供体牛选择、超数排卵、胚胎冷冻保存和分割等相关技术，为牛胚胎移植产业化奠定基础。     

The mechanism of apoptosis induced by homoharringtonine in HL-60 cells

AIM: To study the signal transduction pathway of apoptosis initiation induced by homoharringtonine in HL-60 cells. METHODS: After establishing the model of apoptosis initiation induced by homoharringtonine in HL-60 cells, at the point of apoptosis initiation, molecular caspase-3, Bcl-2, Bax and Fas/FasL were measured with flow cytometry and transmission electron microscope. ERK2 and P38 expression in HL-60 cells were detected by using immunohistochemistry. RESULTS: The model of apoptosis initiation induced by homoharringtonine was established in HL-60 cells. At the point of apoptosis initiation, upregulation of caspase-3 and decrease in Bcl-2/Bax were observed. However, the expression of Fas/FasL did not significantly change. ERK2 expression decreased and P38 expression increased. CONCLUSIONS: Caspase-3, Bcl-2, Bax and mitogen activated protein kinase pathways were involved in signal transduction of apoptosis initiation induced by homoharringtonine in HL-60 cells.     

4种高原乡土禾草的抗旱生理比较

采用盆栽法对梭罗草（Roegneria thoroldiana）、草地早熟禾（Poa pratensis）、垂穗披碱草（Elymus nutans）和中华羊茅（Festuca sinensis）4种高原乡土禾本科牧草幼苗的抗旱性进行了比较。结果表明,一定程度的干旱胁迫后,4种禾草的游离脯氨酸含量、可溶性蛋白含量、可溶性糖含量、过氧化氢酶活性、过氧化物酶活性等均有所增加,且叶绿素分解、细胞膜透性增大、电解质外渗率增加,牧草组织细胞受到伤害。不同牧草在干旱胁迫下生理生化指标的变化不同,综合各项指标,运用隶属函数分析表明,4种禾草抗旱性强弱的排序为梭罗草>草地早熟禾>垂穗披碱草>中华羊茅。     

长株潭耕地土壤pH值分布特征及其与土壤养分的关系

pH值是土壤重要属性之一,研究耕地pH值的分布及其与土壤主要养分之间的关系,对土壤改良和合理高效施肥具有重要参考作用。研究利用统计分析以及地理空间分析技术,分析了长株潭地区耕地土壤pH值分布情况,及不同pH值土壤养分含量的差异状况。结果表明,长株潭耕地pH值以湘潭县最高,炎陵县最低;土壤类型中的pH均值依序为:紫色土潮土水稻土黄壤红壤黄棕壤;除有效磷外,各土壤养分在不同pH值组分间均有显著差异。     

Age-related changes in the anatomical characteristics of Peyer's patches in small intestine of Bactrian camels (Camelus bactrianus)

The distribution, size, and appearance of Peyer's patches vary according to species. In order to determine the anatomical characteristics of Peyer's patches in small intestine of Bactrian camel, and age-related changes in the number of Peyer's patches, 40 Bactrian camels of the following four age groups were studied: young (0.5–2 years), pubertal (3–5 years), middle-aged (6–16 years), and old (17–20 years). The exact number of Peyer's patches was recorded, and the appearance of Peyer's patches was described in detail. The results indicated that Peyer's patches of Bactrian camels not only have a particular anatomical location and distinct appearance but also change with age. They were distributed in the whole small intestine and there were four distinct types of Peyer's patches: nodular, faviform, cup-shaped, and cystic form Peyer's patches. However, the nodular and cystic form Peyer’s patches are specific to Bactrian camel, which have not been found in other animals including Dromedary camel. In addition, the distribution density of Peyer's patches in ileum was the maximum, then was jejunum and duodenum. Further statistical analysis showed that the number of Peyer's patches was altered with age. The number peaked in 5-year-old camels and declined subsequently with age. However, there was little change in the size of Peyer's patches in different age groups; no age-related macroscopic variations in the shape or size of the Peyer's patches were found. Results obtained from this study provide the basic information to further study on the gastrointestinal mucosal immunity of Bactrian camel.     

从实验室能力考核看试验的精确度控制

以本实验室参与的两次果蔬农残能力考核情况为例,通过对实验室两次考核结果的精确度控制的比较,对引起试验误差、导致试验质量失控的原因进行剖析,为农产品质量安全检测实验室的质量控制工作提供参考。