棉铃虫CYP4家族基因片段克隆及序列分析

以5龄实验室品系棉铃虫的总RNA为模板,采用一对昆虫细胞色素P450简并引物,利用反转录—多聚酶链式反应(RT-PCR)扩增并筛选出10个新的长度约450bp的cDNA片段,并对这些序列与GeneBank中已知序列进行相似性分析,初步推测这10个P450基因片段属于CYP4家族的CYP4M和CYP4S2个亚家族。     

FA旱地龙提高水稻旱育秧苗抗旱能力的效果与生理基础

Ｆ Ａ 旱地龙能减缓土壤缺水干旱胁迫下秧苗叶片相对含水量的减少,明显提高秧苗的抗旱性。在干旱胁迫过程中,经 Ｆ Ａ 旱地龙处理的秧苗叶片中 Ｓ Ｏ Ｄ、 Ｃ Ａ Ｔ 活性和抗坏血酸、脯氨酸的含量明显高于对照; Ｍ Ｄ Ａ 含量则低于对照。这些结果表明,减缓干旱胁迫条件下秧苗叶片 Ｓ Ｏ Ｄ、 Ｃ Ａ Ｔ 活性和抗坏血酸含量的降低,促进脯氨酸的积累,减少 Ｍ Ｄ Ａ 的积累,是 Ｆ Ａ 旱地龙提高旱育秧苗抗旱能力的重要生理基础。     

玉米鼠耳病症状识别与发病因素调查

1999年一种玉米“新病害”在贵州省大方等县爆发流行。为了给防治此害提供理论依据,通过借助国内外报道过的有关资料,对此病进行了诊断。诊断结果为玉米鼠耳病。并在发病重的地域进行了发病因素调查。调查结果为（1）玉米品种间存在着抗病性差异;（2）玉米与小米套种的地块发病重;（3）同一乡（镇）内低海拔地区发病早而重;（4）玉米地周围杂草多发病重;（5）播种期适当提早并加盖地膜的发病轻。根据发病因素,提出了防治意见。     

厦门高崎国际机场鸟类调查及鸟撞防治的对策

厦门机场及周边活动的常见鸟类共有30科，90种，在整个锥形面内对飞机飞行构成危险的鸟类，主要是白鹭Egretta garzetta，中白鹭E.intermedia，普通鸬鹚Phalacrocorax carbo，黑翅鸢Elanus caeruleus，山斑鸠Streptopelia orientalis，家鸽等大型鸟类；根据各种鸟类习性及其栖息生境的特点分析，提出了以鸣枪，放煤气炮等方式驱赶鸟类。改变鸟类栖息生境以降低鸟类的种群数量等防止鸟撞的对策。     

内江市城市热岛效应时空演变特征遥感分析

利用内江市1988年6月23日、2000年5月10日和2007年5月6日的Landsat TM/TM＋遥感影像提取了3个时期的城市范围信息,并定量反演了亮度温度,并利用均值-标准差法对亮温进行分级,在此基础上分析了内江市区城市热岛效应的时空演变规律。结果表明,1988～2000～2007年间,内江市区存在明显的城市热岛效应,并呈现出先增强再减弱的趋势。     

Effects of ZIP14 on biological behaviors of hepatocellular carcinoma cell line BEL-7404

AIM: To study the expression of zinc transporter ZRT/IRT-like protein 14 (ZIP14) in the hepatocellular carcinoma (HCC) tissues, and to investigate the effects of ZIP14 over-expression on the biological behaviors of HCC cells. METHODS: The expression of ZIP14 at mRNA and protein levels in the HCC tissues and adjacent non-tumor tissues were detected by real-time PCR and immunohistochemical staining, respectively. The lentivirus expression system containing GV365-ZIP14 was constructed, and was used to infect the HCC cell line BEL-7404, which had relatively poor expression of ZIP14. The expression of ZIP14 at mRNA and protein levels in the transfected cells were detected by real-time PCR and Western blot, respectively. Under the conditions of zinc sulfate stimulation at different concentrations, the cell viability, the cell cycle, and the cell migration and invasion abilities were detected by MTT assay, DNA ploid detection, and Transwell assay, respectively. RESULTS: The mRNA expression level and the strong-positive rate of protein expression of ZIP14 in the HCC tissues were significantly lower than those in the adjacent non-tumor liver tissues (P<0.01). The expression of ZIP14 at mRNA and protein levels in the BEL7404 cells was significantly enhanced by infection of GV365-ZIP14 expression lentivirus. Compared with negative control group (transfected with negative control lentivirus), the cell viability, migration and invasion in ZIP14 over-expression group (transfected with GV365-ZIP14 expression lentivirus) were significantly reduced, and the percentage of the cells in G₂/M phase was significantly increased, all of which were more obvious with the elevation of zinc concentration in the culture medium. CONCLUSION: ZIP14 is low expressed in the HCC tissues. The ZIP14 over-expression has inhibitory effects on the viability, migration and invasion of HCC cells, and blocks the cell cycle in G₂/M phase, which might be closely related to the elevation of zinc concentration in cytoplasma of HCC cells due to enchanced zinc transport by ZIP14.     

东北三省大豆种质品质性状鉴评与综合分析

根据目标性状有的放矢的选配杂交亲本是提高优异品质组成品种的选择效率的基本前提。本研究对东北三省102份大豆种质资源的蛋白、氨基酸组分、油份及脂肪酸组分进行测定,通过遗传多样性、主成分和聚类分析,对其进行表型鉴定及基因型分类以综合评价种质品质特性。结果表明:东北三省大豆种质油份及脂肪酸组分变异较丰富,遗传多样性程度较高。根据主成分分析筛选到9个主成分进行聚类分析,通过聚类分析将供试种质资源分为5类。第I类群蛋白含量较高、油份含量偏低,第II类群蛋白、油份含量均居中,第III类群油份含量较高、蛋白含量偏低,第IV类群高油,第V类群高蛋白,类群间的氨基酸、脂肪酸组分各有差异。需根据育种目标在群体间选配亲本,以提高品质育种的效率。     

玉米根部萜类合酶基因对干旱与激素处理的表达差异分析

选用两个具有抗性差异的玉米自交系B73与Mo17,分别对其根部进行干旱、脱落酸(ABA)、茉莉酸甲酯(MeJA)以及茉莉酸甲酯与乙烯利联合(MeJA/EP)处理,定量检测4个与防御相关的萜类合酶基因An2、ZmTPS6、ZmTPS8以及ZmTPS26的表达情况。结果表明,4个基因在B73与Mo17应对干旱和ABA处理时基因表达差异明显。在Mo17中,干旱胁迫以及ABA处理显著诱导4个基因的表达;在B73中,干旱处理导致An2、ZmTPS6和ZmTPS8基因表达下调,在ABA处理下时,只有ZmTPS6被显著上调;MeJA与MeJA/EP处理在B73与Mo17中都能上调4个基因的表达,但在Mo17中的诱导程度更剧烈。B73与Mo17的根部在响应胁迫时存在显著的萜类代谢差异,可能为其抗性差异原因之一。     

六堡茶种质资源调查初报

通过对六堡茶种质资源进行调查,发现六堡茶品种资源在苍梧全县、藤县金鸡镇、岑溪市南渡镇等地均有分布,以苍梧县六堡镇、狮寨镇为主要分布地。这些茶原料的水浸出物含量为46.6%~56.4%,茶多酚含量为32.2%~49.7%,氨基酸含量为2.7%~5.2%,咖啡碱含量为2.7%~6.6%,儿茶素含量为17.5%~24.6%,适制六堡茶和红茶。     

抗逆樱桃番茄新品种‘红樱1号’

 樱桃番茄新品种‘红樱1号’是以自交系‘SH-0-2-0-1’为母本,‘TC-0-1-3-1’为父本杂交而成的一代杂种。无限生长类型,生长势强。成熟果深红色,椭圆形,单果质量约20 g,耐裂果,品质优。耐热,耐寒,耐旱性强,适应性广,适宜于华南地区春、秋季栽培。