丽江云杉家系苗期性状通径分析与优良家系初选

对91个丽江云杉(Picea likiangensis)2年生实生苗的苗高、地径、侧枝数、根系长和干重5个生长性状进行遗传分析和通径分析,并采用综合选择指数法对丽江云杉进行优良家系的评选。结果表明：2年生丽江云杉实生苗家系间各性状存在极显著差异(P＜0.01),其中侧枝数的遗传力最大,根系长的遗传力最小;性状的遗传变异系数干质量最大,苗高最小;地径对苗高的直接贡献最大,根系长通过地径对苗高的间接作用最大。通过综合选择指数法,评选出12个优良家系,这些家系的苗高实际增益为13.10%～29.58%,遗传增益为11.17%～25.24%。     

基于高通量测序的数字基因表达谱技术研究进展

数字基因表达谱(digital gene expression profiling,DGE)是一种全面、快速地检测某一物种特定组织在特定状态下基因表达情况的高通量测序技术。通过对基因表达谱的生物信息学搜索、比较和分析,可从中获取基因转录、基因调控、信号转导通路、蛋白质功能等相关信息。该研究主要介绍了数字基因表达谱的原理以及在生物学和医学研究等方面的应用。     

不同播期、种植密度及整枝方式对黄秋葵产量的影响

为在甘肃庆阳地区推广种植黄秋葵,在塑料大棚内进行了4个播期(A)、2个种植密度(B)、2种整枝方式(C)对黄秋葵生长及产量影响的试验。试验结果表明,影响黄秋葵产量的关键因素是播期,其次是种植密度,整枝方式对产量影响不大,最优组合是A2B1C2,即播期3月30日、株行距30 cm×50 cm、不整枝,以及A1B1C1,即播期3月15日、株行距30 cm×50 cm、单秆整枝。     

东北苹果产区抗寒砧木研究与利用进展

该文针对我国东北苹果栽培区域的不同气候特点,总结了各地栽培现状,在综合前人研究成果的基础上,概述了不同气候区的苹果主栽品种和主要砧穗组合;对苹果主要砧木资源、抗寒性研究方法及应用现状进行综述,并对抗寒砧木的利用提出建议。     

Effect of gold nanoparticles on reversing adriamycin resistance of human hepatocellular carcinoma HepG2/ADM cells

AIM: To investigate whether gold nanoparticles (GNPs) reverses adriamycin (ADM), resistance of human hepatocellular carcinoma drug-resistant cell line HepG2/ADM and to explore the potential mechanism. METHODS: The sensitivities of HepG2 cells and HepG2/ADM cells to ADM were tested by MTT assay before and after GNPs pretreatment. The apoptotic rate was examined by flow cytometry. The concentration of ADM in HepG2/ADM or HepG2 cells was determined by ultraviolet-visible spectrophotometer. The content of glutathione (GSH) in HepG2/ADM or HepG2 cells by DTNB method. RESULTS: The half maximal inhibitory concentrations (IC₅₀) of ADM for HepG2/ADM cells were(29.46±1.73) mg/L and (15.18±0.85) mg/L before and after GNPs pretreatment,respectively. The IC₅₀ of ADM for HepG2 cells was (9.16±2.03) mg/L before pretreatment. The apoptotic rate in GNPs+ADM group was higher than that in ADM group (P<0.05). The concentration of ADM in HepG2/ADM group was lower than that in HepG2 group (P<0.01). After GNPs pretreatment, the concentration of ADM in HepG2/ADM cells was higher than that before pretreatment. The content of GSH in HepG2/ADM group was higher than that in HepG2 group (P<0.01). After GNPs pretreatment, the content of GSH in the HepG2/ADM cells was lower than that before pretreatment. CONCLUSION: Gold nanoparticles can reverse ADM resistance of human hepatocellular carcinoma drug-resistant cell line HepG2/ADM, reduce the content of GSH and increase the concentration of ADM in HepG2/ADM cells.     

豫西黄土丘陵区生态环境条件分析与生态果园复合模式研究

为了更好地在豫西黄土丘陵区建设生态果园,分析了该区气候、地貌、土地、植被及水土流失等生态环境条件,介绍了"草-牧-沼-窖-果"、"草-牧-果"和以旅游观光为主的3种生态果园复合模式。并对生态果园建设中果园的规划和布局、水土保持措施建设、保水耕作技术及生物防治技术进行了分析和探讨,以期为生态果园建设提供理论基础和生产指导。     

广西野生鳞盖红菇原种培养研究

为获得野生鳞盖红菇原种,利用通过组织分离获得的母种,根据母种分离培养经验并利用各种食用菌原种培养料搭配成多种配方培养红菇原种。结果表明:花生饼对原种培养有较大促进作用;麦子较适合作为培养原种主原料;配方棉籽壳15%、麦麸10%、麦子57%、玉米粉8%、花生饼8%、蔗糖0.8%、石膏1%、硫酸镁0.2%能长出较合格原种。     

喀斯特山区野生燕麦种子发芽特性研究

喀斯特山区野生燕麦种子最适萌发温度为15℃、20℃和30℃,英种子的发芽势与发芽率均高这80％以上;20℃种子萌发条件下,浸泡96 h后5℃低温处理下的种子能达到最佳发芽势与发芽率,分别为92％、90％.30℃种子萌发条件下,浸泡72 h后5℃低温处理下的种子能达到最佳发芽势与发芽率,分别为82％、80％.在25℃种子催芽条件下,浸种8h其种子的发芽势和发芽率均达到最佳,分别为72％和76％.     

Signal Processing of Electroencephalogram and It''s Application

Electroencephalogram (EEG) signals are the electrical activities in the cortex or on the surface of scalp caused by the physiological activities of the brain which play a key role in the diagnosis of brain and the functional determination of brain. The authors discuss some methods for processing EEG signals from the view of the frequency domain and time domain, especially introduce some time-frequency analysis methods such as Wigner distribution, wavelet transform and matching pursuit etc. , ANN and non-linear analysis for EEG signals processing.     

Researching and Developing a Hybrid Electrical Vehicle

There are more than two power source in Hybrid Electrical Vehicle(HEV). By using of assist accelerating, power regeneration, Idle stop and power management, HEV gains more acceleration performance and more fuel economy and better gas emission than Conventional Vehicle(CV). With Integrated Starter and Generator(ISG) as a auxiliary power source, batteries and battery control module and power manage module as a Load leveling Device (LLD), two prototype HEV separately equipped Manual Transmission (MT) and Continuously Variable Transmission (CVT) was successfully developed.