60%吡唑醚菌酯·代森联水分散粒剂防治猕猴桃褐斑病田间药效试验

2017年开展的60%吡唑醚菌酯·代森联水分散粒剂防治猕猴桃褐斑病田间药效试验,结果表明,该药剂对猕猴桃灰霉病有很好的防治效果,于叶片发病初期使用有效剂量400~600mg/kg,连续茎叶喷雾2次,10d后防治效果达75%~83%,试验剂量下对猕猴桃树生长安全。     

梨不同砧穗组合光合特性

 用CIRAS-1 便携式光合作用测定系统研究了豆梨(Pyrus betulaefolia Bunge) 、杜梨(P.calleryana Dcen.) 幼树以及豆梨、杜梨与砂梨(P. pyrifolia Nakai) 品种丰水、新高4 种砧穗组合幼树叶片的光合作用。结果表明, 砧木显著影响栽培品种的光合作用, 杜梨比豆梨以及以杜梨为砧木的丰水、新高幼树比以豆梨为砧木的两品种幼树叶片净光合速率(Pn) 、表观量子产量(Φi) 、羧化效率(CE) 、水分利用效率(WUE) 高。新高的Pn、Φi 、CE 和WUE 值较丰水高, 且在两种砧木上均表现出同样的结果。     

木本婆婆纳‘维力镜像’的组培快繁技术

木本婆婆纳（Hebe）原产澳洲,主要分布于新西兰和澳大利亚的东南部,全属约100种,栽培品种超过800个,是一类观赏常绿花灌木.在澳洲和欧洲,被广泛应用在观赏园艺和庭院绿化上,并对Hebe属植物的育种和栽培做了大量的研究.近年来,我国也开始逐步认识和引种Hebe属植物,并有少量用于生产,但组培方面的研究国内尚未见报道.     

桂林老虎猫瘟热病毒的分离鉴定

我们在作猫细小病毒分子流行病学调查过程中,从桂林送棼的考虑粪便中分离出１株虎细小病毒,并对其进行了系统鉴定,经形态学理化学血清学交叉中和试验、动物感染试验与分子生物学,证明为一株猫瘟热病毒（猫泛白细胞减少症病毒）的强毒。     

果菜类蔬菜早熟性育种研究进展

早熟性是蔬菜作物育种的重要指标,对农业增产和农民增收意义重大。本文对果菜类蔬菜早熟性评价指标,早熟性相关农艺性状和早熟性育种方法三个方面的研究进展进行综述,并对基因工程辅助早熟育种进行了展望。     

Effects of dietary oregano essential oil supplementation on growth performance,intestinal antioxidative capacity,immunity, and intestinal microbiota in yellow-feathered chickens

Essential oils are plant-derived aromatic volatile oils, and they contain bioactive compounds that have been shown to improve poultry nutrition. In this study, we investigated the effects of oregano essential oil (OEO) on intestinal antioxidative capacity, immunity, and gut microbiota of young yellow-feathered chickens. A total of nine hundred and sixty 1-d-old female Qingyuan partridge chickens were randomly allocated to four treatment groups with six replicates of 40 birds each, and the feeding trial was lasted for 30 d. The controls were fed on a basal diet without in-feed antibiotics; the birds in the antibiotic group were fed the basal diet supplemented with 20 mg/kg virginiamycin; the remaining birds were fed the basal diet containing 150 or 300 mg/kg OEO, respectively. Dietary supplementation with 150 or 300 mg/kg OEO increased average daily feed intake (P = 0.057) and average daily gain (P < 0.05). The activities of glutathione peroxidase and total antioxidative capacity in plasma, jejuna, and ileal mucosa were increased by OEO supplementation (P < 0.05), with a trend of lower jejunal content of malonaldehyde (P = 0.062). Moreover, dietary OEO increased the content of secretory immunoglobulin A (P = 0.078) and the relative expression of Claudin 1, Mucin 2, and Avain beta-defensin 1 in ileum (P < 0.05). Sequencing data of 16S rRNA indicated that dietary OEO increased the relative abundance of Firmicutes phylum, and Clostridium and Lactobacillus genera, and decreasing that of Romboutsia. Functional analyses indicated that microbial amino sugar and nucleotide sugar metabolism, replication, and repair systems were higher in OEO groups than those of controls and antibiotic treatment. In conclusion, dietary supplementation with OEO enhanced growth performance, alleviated local oxidative stress in intestine, improved production of natural antibodies, and favorably modulated intestinal microbiota composition.     

Free choice feeding of whole grains in meat-type pigeons: 1. effect on performance,carcass traits and organ development

1. The effects of 5 different feeding systems on the performance, carcass traits and organ development were studied in pigeon squabs.

2. The 5 treatments were (1) whole grains of maize, pea and wheat plus concentrate feed; (2)whole grains of maize and wheat plus concentrate feed (CWC); (3) whole grains of maize and pea plus concentrate feed; (4)whole grain of maize plus concentrate feed (CC); and (5) compound feed (CF).

3. Feed intake of parent pigeons increased significantly from 0 to 21 d and it was higher in the CF treatment. Body weight of squabs in the CWC treatment was the highest among the 5 treatments in 4 weeks. Body weight losses of parental pigeons during the rearing period were not significantly different among the 5 treatments.

4. Protein intake in CC and CWC treatments was lower than that of the other three treatments. The CWC treatment had the highest daily weight gain and the lowest feed conversion ratio. Treatments were statistically similar in the relative weight of carcass, breast and thigh. CF had the lower relative weight of abdominal fat.

5. Relative weight of gizzard in the CF treatment was significantly lower than that of CWC.

6. It was concluded that the application of free choice feeding of whole grains of maize and wheat plus concentrate feed increased the body weight of 28-d-old pigeon squabs and decreased the feed conversion rate of parent pigeons. This feeding strategy could be commercially interesting in meat-type pigeon production.

     

二化螟dsRNA降解酶基因的克隆与表达分析

为解析二化螟Chilo suppressalis体内参与降解双链RNA(double-stranded RNA,dsRNA)的关键核酸酶的功能,克隆二化螟不同的非专一性核酸酶（non-specific nuclease,NUC）基因,并对这些基因进行生物信息学分析和组织定量表达分析,同时对dsRNA降解酶（dsRNA degrading nuclease,dsRNase）活力的组织分布进行研究。结果显示,共克隆获得5个NUC基因,其中有4个编码dsRNase亚家族基因（CsdsRNase1～CsdsRNase4）和1个编码Endonuclease G亚家族基因（CsEndoG）。5个NUC基因的开放阅读框核苷酸序列长度范围为828～1 338 bp,编码275～445个氨基酸残基,其分子量大小为31.68～49.57 kD,预测等电点为5.48～9.42。CsdsRNase1和CsdsRNase2含有信号肽序列,两者相似度极高,且与家蚕Bombyx mori和斜纹夜蛾Spodoptera litura中具有dsRNA降解酶活力的dsRNase同源聚类;CsdsRNase1和CsdsRN...     

我国农业可持续发展的理论与实现措施

目前,现代农业的发展面临着一系列的困难问题:发达国家主要是环境污染、农业投入增加、成本过高;发展中国家主要是人口膨胀、资源破坏和过渡消耗、食物安全问题严重。为此,从持续农业的产生背景出发,提出了持续农业的基本内容和主要目标。针对我国国情,从几个方面论述了我国实现可持续农业需要的政策措施。     

时滞半主动悬架模糊神经网络控制

建立了含时滞半主动悬架数学模型,分析了悬架系统产生时滞的原因,设计了含时滞补偿模糊神经网络自适应控制策略,开发了C8051单片机为内核的半主动悬架控制系统,仿真分析后,在全真半主动悬架试验台上进行了台架试验。结果表明,实施半主动悬架神经网络时滞补偿控制,解决了控制中的“不合拍”问题,较好地协调了车辆舒适性和安全性之间的矛盾。