Comparison of different conditions inducing embryonic stem cells

AIM: To evaluate the different conditions inducing mouse embryonic stem cells (ESC) in vitro to differentiate into cardiomyocytes. METHODS: BRL conditioned medium was used to promote the growth of ESC and maintain them in an undifferentiated state. During the inducing process, retinoic acid (RA), DMSO, activin-A and TGF-β₁ were used as inducing reagents, and made up six kinds of differentiating medium. Then a three-step method inducing ESC cultured in hanging drops, in suspension and in plating was used to induce the differentiation of ESC. RESULTS: ESC were induced in vitro to differentiate into cardiomyocytes. Of all groups, the highest differentiating rate was observed in the group induced by activin-A (20 μg/L) and TGF-β₁ (2 μg/L). CONCLUSION: The inducing conditions including activin-A (20 μg/L) and TGF-β₁ (2 μg/L) is very valuable in inducing ESC differentiation into cardiomyocytes.     

噻二唑基-3-哒嗪酮类化合物的合成及生物活性

将取代的二酰基肼环合后,得到中间体2-芳基-5-氯甲基-1,3,4-噻二唑,然后与2-叔丁基-4-氯-5-羟基-3(2H)-哒嗪酮反应,合成了8个未见文献报道的含噻二唑基哒嗪酮类化合物,其化学结构经1H NMR、高分辩质谱和元素分析确认。生物活性测试结果表明,部分化合物对粘虫P.separate W.有较好的抑制生长活性,其中化合物 3b 的EC50值为21 mg/L。     

生姜癞皮病的发生危害及病原初步鉴定

生姜癞皮病在山东7月开始发病,8月中旬至9月中旬为盛发期。病株植株矮小、茎细、分枝少,叶色变淡,根茎表皮疣裂,根系腐烂,产量品质降低。病原物为南方根结线虫。砂质土壤、连作重茬、过量施钾肥均可加重该病的发生。     

Bone marrow mesenchymal stem cells are induced to differentiate into cardiomyocytes by hepatocyte growth factor in vitro

AIM: To explore a new method of hepatocyte growth factor (HGF) inducing bone marrow mesenchymal stem cells (MSC) to differentiate into cardiomyocytes. METHODS: Bone marrow MSC was cultured with DMEM media (10% fetal calf serum) 4-6 passages, and induced by HGF (10 μg/L) for 30 d. Automatical beating of the differentiated cells was observed daily with transverse microscopy, or under condition of 0.1% isoproterenol or cal-cium-deprived incubation. Specific cardiac myosin in the cells was indentified by immunochemistry. RESULTS: At 14-20 d of differentiation, bone marrow mesenchymal stem cells formed clones, in 10%-50% of which spontaneous beating cell-mass had come to continuously exist. Isoproterenol increased the beating rate and calcium-deprived media inhibited the beating. The cells were identified to be cardiomyocytes by expression of cardiac myosin heavy chain. CONCLUSION: HGF may induce bone marrow mesenchymal stem cells into cardiomyocytes with high efficiency, but the differentiating pathway of stem cells remains to be further studied.     

表达口蹄疫病毒P1基因的重组伪狂犬病病毒TK/gG-/PgG-P1的构建

为了构建口蹄疫与伪狂犬病二价基因工程疫苗株 ,将口蹄疫病毒 (FMDV) P1基因插入到伪狂犬病病毒 (PRV)通用载体 p Pg G- uni中 ,得到 PRV转移载体 p Pg G- P1。将转移载体与 Eco R 线性化后的 PRV弱毒疫苗株 TK- /g G- / lac Z 基因组 DNA共转染 PK- 15细胞 ,转染产物经多次空斑纯化和 PCR鉴定 ,获得了纯化的重组 PRV TK- /g G- / Pg G- P1,重组病毒基因组 DNA经酶切鉴定进一步表明 ,FMDV的 P1基因已成功地整合到 PRV弱毒疫苗株的基因组中。Western blot试验表明 ,FMDV P1基因在重组 PRV中得到表达。该研究为进一步研制口蹄疫与伪狂犬病二价基因工程疫苗奠定了坚实的基础     

Comparative studies of acetylcholinesterases purified from two field populations of the oriental migratory locust (Locusta migratoria manilensis): implications of insecticide resistance

Acetylcholinesterase (AChE) was purified by affinity chromatography from two populations of the oriental migratory locust, Locusta migratoria manilensis (Meyen), collected from Huanghua and Pingshan Counties, Hebei Province of China. The purification factors and yields were 1661-fold and 19.3%, respectively, for the Huanghua population, and 3897-fold and 39.6% for the Pingshan population. Both the purification factor and yield were significantly lower in the Huanghua population than in the Pingshan population. AChE activity was almost completely inhibited by 10⁻⁶ M eserine and BW284C51, but ?5.8% of AChE activity was inhibited by ethopropazine at the same concentration, suggesting that purified AChE from either population was a typical insect AChE. However, AChE purified from the Huanghua population was 62-, 2.0-, and 1.6-fold less sensitive to inhibition by the three organophosphate compounds, chlorpyrifos oxon, demeton-S-methyl, and paraoxon, respectively, than that from the Pingshan population. Significantly lower purification factor and low yield associated with reduced sensitivity of AChE to inhibition by the organophosphates indicated that AChE purified from the Huanghua population was biochemically and pharmacologically different from that of the Pingshan population. Reduced sensitivity of AChE appeared to contribute to organophosphate resistance in the locust from Huanghua County, where insecticides have commonly been used to manage outbreaks of the locust.     

华北农牧交错带退化草地土壤种子库动态变化

对华北农牧交错带不同退化演替阶段土壤种子库的数量和物种组成及与地上植被的关系进行了初步的研究,结果表明:农牧交错带草地在刈割和放牧等干扰下,发生逆行演替,土壤种子库中植物种类及种子数量呈递减的趋势,轻度退化类型土壤种子库物种组成最高,有23种植物,种子数量最多,有4 303粒,禾本科所占比例较大,为32.3%;而极度退化草地物种组成最低,有14种植物,种子数量仅有1 156粒,禾本科只占8.4%.草地逆行演替过程中植被与土壤种子库在物种组成上存在一定差别,中度退化阶段差别最大,有18种植物未在种子库中出现,极度退化阶段差别最小,有2种未在种子库中出现.     

短期禁牧对天山北坡蒿类荒漠群落特征及其稳定性的影响

本研究以天山北坡蒿类荒漠为研究对象,采用野外调查取样的方法,探讨不同区域荒漠围栏内外群落特征和物种多样性对禁牧的响应规律,分析禁牧对生态系统稳定性的影响。结果表明,1)禁牧能改变荒漠植被功能群结构,禁牧后不同生态经济类群群落盖度和生物量分别上升40.2%~57.0%和44.4%~81.0%;2)荒漠群落物种多样性指数对短期禁牧的响应较小,但从区域上看,玛纳斯荒漠放牧区Patrick丰富度指数、Shannon-Wiener多样性指数比博乐和奇台显著高16.7%~20.0%,而禁牧区Simpson优势度指数、Shannon-Wiener多样性指数、Pielou均匀度指数高了8.6%~17.7%;3)短期禁牧有利于荒漠群落稳定性的维持,且稳定性可能与禁牧年限和生态环境的变化均有一定的关系。因此,短期禁牧能显著提高荒漠草地生产力,有利于群落物种多样性和群落稳定性的维持。     

干旱区工业废渣粉煤灰回填材料拌合物的流动性能研究

针对干旱区存在大量的粉煤类废弃物及回填工程中作业面受限或结构物死角存在压实不到位的工程问题,提出以粉煤灰为主要成分、以及部分砂、水泥的流动化回填材料,分析拌合物流动性能受材料掺量、时间影响的变化规律,建议采用坍落扩展度作为流动性能评价指标,水泥掺量对流动性能影响甚微,坍落扩展度与水固比成线性关系;同一种集料,不同粉煤灰掺量拌合物达到相同的流动性能,水固比呈先降后升的变化趋势;拌合物坍落扩展度在前60min内降低显著,随时间变化,坍落扩展度趋于一致,拌合物中集料颗粒越小,保持流动性的能力越强.     

不同竹龄、部位竹材经软化后的力学性能比较研究

以毛竹为研究对象,对3个竹龄(1.5、3.5和5.5年生)和5个纵向高度段竹材进行水煮软化处理,比较研究水煮软化后不同年份,不同部位竹材顺纹抗拉强度,顺纹抗压强度,静曲强度和抗弯弹性模量的变化情况,以了解其变化规律。结果表明:1在水煮软化循环实验处理下,毛竹的顺纹抗压强度、顺纹抗拉强度,抗弯弹性模量均呈现下降趋势;2从竹龄来看,在水煮软化循环作用下,5.5年生的毛竹材的各项性能变化差异最小,性能较稳定;1.5年生的毛竹材的性能变化差异最大,较不稳定。3从5个纵向高度看,毛竹材自下而上的顺纹抗压强度、顺纹抗拉强度的变化差异逐渐减小,即毛竹的基部的性能的变化最大,较不稳定,梢部的性能变化最小,较稳定。