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91.
C. Guillermo Couto José J. Cerón MD Parra Silvi Martínez‐Subiela M. Cristina Lazbik 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2009,38(2):219-223
Background: Retired racing Greyhounds are popular as pets. Greyhounds have several differences in physiological values compared with other breeds, including lower serum α‐ and β‐globulin concentrations. We hypothesized that lower acute phase protein (APP) concentrations could contribute to lower α‐ and β‐globulin concentrations in this breed. Objectives: The purpose of this study was to compare serum concentrations of several APPs in Greyhounds with those of other dog breeds. Methods: We measured the serum concentrations of C‐reactive protein (CRP), haptoglobin (Hp), acid‐soluble glycoprotein (ASG), ceruloplasmin (CP), and serum amyloid A (SAA) in 15 clinically healthy retired racing Greyhounds and 11 age‐ and gender‐matched healthy nonGreyhound controls using previously validated methods. Results were compared by Student's t‐tests. Results: The concentration of Hp by both colorimetric and immunoturbidimetric methods was significantly lower in Greyhounds than in nonGreyhound dogs (P=.0009 and .019, respectively). The concentration of ASG was also significantly (P=.007) lower in Greyhounds, but CRP and CP concentrations were not significantly different between groups. SAA concentration was below the detection limit of the method in all dogs. Conclusions: The low serum concentrations of Hp and ASG should be taken into consideration when interpreting APP results in Greyhounds. Because both Hp and some ASG migrate in the α‐globulin fraction, these results may explain the low α‐globulin concentrations in Greyhounds. 相似文献
92.
Leandro Maia MV MS Maria Vernica de Souza MV MS DS Pharmacist-Biochemical Jos Ivo Ribeiro Júnior BS MS DS Acio Carlos de Oliveira Geraldo Eleno Silveira Alves MV MS DS Larcio dos Anjos Benjamin MV MS DS Yamê Fabres Robaina Sancler Silva Bruna Mota Zandim MV Jos do Carmo Lopes Moreira MD MS 《Journal of Equine Veterinary Science》2009,29(8):618-626
This study was carried out to evaluate the effect of platelet-rich plasma (PRP) on the treatment of tendinopathy induced in the superficial digital flexor tendon (TFDS) of horses, by using histologic evaluation. Six healthy crossbred geldings aged 8 to 15 years (12 ± 3) were used. The TFDS tendinopathy was provoked in both forelimbs, by intratendinous administration of 2.5 mg collagenase (2.5 mg/mL), and this procedure was considered as the beginning of the experimental phase. At 12 days after induction of the tendinopathy, the animals were subjected to the following treatments: (1) in the lesion caused in the right superficial digital flexor tendon (PRP-treated group), 2.5 mL PRP activated with calcium chloride at 0.0125 mol/L at concentrations from 320,000 to 500,000 platelets/μL, were injected; (2) in the tendinopathy of the left SDFT (control group), 2.5 mL 0.9% saline solution was administrated. Thirty-six days after the treatments, a biopsy of the injured area was performed for histologic evaluation. In both groups, the histologic analysis showed an increase in the fibroblastic density, as well as the presence of neovascularization, lymphocytes, and plasmocytes infiltrate and tissue organization at variable intensity. In the PRP-treated group, the SDFT was more organized, with the collagen fibers and fibroblasts being better arranged on the tendon matrix. The numbers of the fibroblasts and blood vessels did not differ between the groups. Histologic evaluation 36 days after tendinopathy showed that injuries under a single PRP treatment present a more uniform and organized tissue repair when compared with the control group. 相似文献
93.
Daniel Schenkman DVM PhD Anibal Armien DVM PhD Roy Pool Jr. DVM PhD James M. Williams PhD Ronald D. Schultz PhD Jorge O. Galante MD 《Journal of Equine Veterinary Science》2009,29(10):748-752
Recently Degenerative Suspensory Ligament Desmitis (DSLD) has been proposed to be a disease characterized by systemic deposition of proteoglycan (PG) in connective tissues. To investigate this hypothesis, 6 clinically affected Peruvian Paso horses were compared to 2 unaffected quarterhorses and one unaffected standardbred. Histological sections of limb ligaments and tendons, nuchal ligaments, aortas, hearts, eyes, visceral organs and brains from both groups were stained with H&E as well as special stains for PG. Safranin-O stained sections were found to be optimal for elucidating the presence of PG. Although lesions characteristic of DSLD were present in suspensory ligaments of each clinically affected horse, including foci of chondroid metaplasia with abundant PG, a similar but less pronounced pattern of PG deposition was present in control horses. In contrast to findings of the previous study, PG deposition was not unique to DSLD horses, and PG deposition in aortas and nuchal ligaments of some control horses exceeded levels of PG present in similar tissue of DSLD horses. Furthermore, the “vascular lesion” described in the media of arteries as cellular separation and intercellular amorphous matrix deposition was within the spectrum of changes recognized in both affected and unaffected horses. We found no evidence that DSLD is a systemic PG deposition disease. 相似文献
94.
LA Quintela AI Peña MD Vega J Gullón C Prieto M Barrio JJ Becerra PG Herradón 《Reproduction in domestic animals》2009,44(5):829-833
This study was aimed to evaluate the reproductive performance of rabbit does artificially inseminated (AI) with a GnRH analogue [des‐Gly10, d ‐Ala6]‐LHRH. ethylamide to induce ovulation by intravaginal administration, delivered in the seminal dose. In a preliminary experiment, 39 does were divided into three groups (n = 13) that, at the time of AI, received the following ovulation induction treatments: (i) control group: 20 μg of gonadorelin administered intramuscularly; (ii) 25 μg of the GnRH analogue added to the seminal dose; (iii) 30 μg of the GnRH analogue added to the seminal dose. Fertility did not differ between the three groups (control: 80.6%, group 2: 82.8%, group 3: 73.3%). In a second experiment, a large‐scale field trial was conducted to test the use of 25 μg of the GnRH analogue [des‐Gly10, d ‐Ala6]‐LHRH ethylamide delivered in the seminal dose (n = 270) against 20 μg of gonadorelin administered intramuscularly. Fertility was higher (p < 0.05) when ovulation was induced by intravaginal administration of the GnRH agonist (91.1% vs 85.6%). Prolificacy or mortality at birth was never affected by the ovulation induction treatments. In a third experiment, two groups of does [control group (n = 39): ovulation was induced using 20 μg of gonadorelin administered intramuscularly; treatment group (n = 40): ovulation was induced using 25 μg of [(des‐Gly10, d ‐Ala6)‐LHRH ethylamide added to the seminal dose] were inseminated at 42‐day intervals for five successive AI cycles, to test the response to the GnRH agonist after repeated intravaginal administration to the same animals. Fertility and prolificacy were not influenced by the ovulation induction treatment neither there was an interaction between treatment and parity. The last experiment was aimed to determine whether it could be possible to add the GnRH agonist to the semen in the AI Center, just after semen collection and dilution, or it would have to be added in the farm, immediately before AI. Kindling rates did not significantly differ when ovulation was induced by intramuscular injection of gonadorelin (84.5%) or when the GnRH agonist was added to the seminal dose just at the moment (93.8 %) or 24 h before AI (90.4 %), but it was significantly lower when the hormone was added to the semen 32 h before AI (76.3 %). Prolificacy, however, was not influenced by the ovulation induction treatment. 相似文献
95.
Busani L Valsecchi MG Rossi E Toson M Ferrè N Pozza MD Marangon S 《Veterinary journal (London, England : 1997)》2009,181(2):171-177
In 1999-2000, Italian poultry production was disrupted by an H7N1 virus subtype epidemic of highly pathogenic avian influenza (HPAI). The objectives of the present study were to identify risk factors for infection on poultry farms located in regions that had the highest number of outbreaks (Veneto and Lombardia) and the impact of pre-emptive culling as a complementary measure for eradicating infection. A Cox regression model that included spatial factors, such as the G index, was used. The results confirmed the relationship between risk of infection and poultry species, production type and size of farms. The effectiveness of pre-emptive culling was confirmed. An increased risk of infection was observed for poultry farms located near an infected farm and those at altitudes less than 150m above sea level. The measures for the control and eradication of AI virus infection need to consider species differences in susceptibility, the types of production and the density of poultry farms in the affected areas. 相似文献
96.
97.
Ignacio A Gomez de Segura DMV DrMedVet Diplomate ECVAA Antonella Menafro DMV Paloma García-Fernández DMV DrMedVet Silvia Murillo DMV & Elba M Parodi† MD 《Veterinary anaesthesia and analgesia》2009,36(5):485-494
Objective To compare the analgesic and motor-blocking effects of epidurally administered levobupivacaine and bupivacaine in the conscious dog.
Study design Prospective, randomized, cross-over study.
Animals Six adult female Beagle dogs.
Methods Each animal received three doses of levobupivacaine or bupivacaine (0.5, 1.0 and 1.5 mg kg−1 ; concentrations 0.25%, 0.50%, and 0.75%, respectively) in a total volume of 0.2 mL kg−1 by means of a chronically implanted epidural catheter. Onset, duration (through pinch response in the sacral, lumbar and toe areas) and degree of analgesia and motor-blocking status was determined with a scoring system and at regular intervals over 8.5 hours before (baseline) and after drug administration.
Results Epidurally administered levobupivacaine and bupivacaine had a similar dose-dependent analgesic action with no significant differences in onset (range: 5–8 minutes), duration (bupivacaine: 42 ± 28, 135 ± 68 and 265 ± 68 minutes, and levobupivacaine: 28 ± 33, 79 ± 55 and 292 ± 133 minutes; 0.25%, 0.50%, and 0.75%, respectively) or maximum degree of analgesia. However, levobupivacaine tended to produce a shorter duration of motor block than bupivacaine and the difference in the motor to nociceptive blockade times was significant at the highest dose.
Conclusion Epidural levobupivacaine produced an analgesic action similar to that of bupivacaine.
Clinical relevance Epidural levobupivacaine is suitable for clinical use in dogs, mostly at the highest dose if a high degree of analgesia is required. 相似文献
Study design Prospective, randomized, cross-over study.
Animals Six adult female Beagle dogs.
Methods Each animal received three doses of levobupivacaine or bupivacaine (0.5, 1.0 and 1.5 mg kg
Results Epidurally administered levobupivacaine and bupivacaine had a similar dose-dependent analgesic action with no significant differences in onset (range: 5–8 minutes), duration (bupivacaine: 42 ± 28, 135 ± 68 and 265 ± 68 minutes, and levobupivacaine: 28 ± 33, 79 ± 55 and 292 ± 133 minutes; 0.25%, 0.50%, and 0.75%, respectively) or maximum degree of analgesia. However, levobupivacaine tended to produce a shorter duration of motor block than bupivacaine and the difference in the motor to nociceptive blockade times was significant at the highest dose.
Conclusion Epidural levobupivacaine produced an analgesic action similar to that of bupivacaine.
Clinical relevance Epidural levobupivacaine is suitable for clinical use in dogs, mostly at the highest dose if a high degree of analgesia is required. 相似文献
98.
Mary Beth Callan VMD DACVIM ; Elizabeth H. Appleman VMD DACVIM Bruce S. Sachais MD PhD 《Journal of Veterinary Emergency and Critical Care》2009,19(5):401-415
Objective – To review potential platelet storage options, guidelines for administration of platelets, and adverse events associated with platelet transfusions. Data Sources – Data sources included original research publications and scientific reviews. Human Data Synthesis – Transfusion of platelet concentrates (PCs) plays a key role in the management of patients with severe thrombocytopenia. Currently PCs are stored at 22°C under continuous gentle agitation for up to 5 days. Chilling of platelets is associated with rapid clearance of transfused platelets, and galactosylation of platelets has proven unsuccessful in prolonging platelet survival. Although approved by the American Association of Blood Banks, cryopreservation of human platelets in 6% DMSO largely remains a research technique. Pre‐storage leukoreduction of PCs has reduced but not eliminated acute inflammatory transfusion reactions, with platelet inflammatory mediators contributing to such reactions. Veterinary Data Synthesis – Canine plateletpheresis allows collection of a concentrate with a high platelet yield, typically 3–4.5 × 1011 versus <1 × 1011 for whole blood‐derived platelets, improving the ability to provide sufficient platelets to meet the recipient's transfusion needs. Cryopreservation of canine platelets in 6% DMSO offers immediate availability of platelets, with an acceptable posttransfusion in vivo platelet recovery and half‐life of 50% and 2 days, respectively. While data on administration of rehydrated lyophilized platelets in bleeding animal models are encouraging, due to a short lifespan (min) posttransfusion, their use will be limited to control of active bleeding, without a sustained increase in platelet count. Conclusions – Fresh PC remains the product of choice for control of bleeding due to severe thrombocytopenia or thrombopathia. While cryopreservation and lyophilization of canine platelets offer the benefits of immediate availability and long‐term storage, the compromise is decreased in vivo recovery and survival of platelets and some degree of impaired function, though such products could still be life saving. 相似文献
99.
Nicole C. Sereda DVM Simon Towl DVM Herbert W. Maisenbacher III VMD Mark S. Bleweis MD Julie K. Levy DVM Barry J. Byrne MD Gary W. Ellison DVM Andre Shih DVM Alastair R. Coomer BVSc Amara H. Estrada DVM 《Journal of Veterinary Cardiology》2009,11(1):53-58
A dog that was referred to the University of Florida Veterinary Medical Center was discovered to have a bamboo skewer within the right atrium and right ventricle, traversing the tricuspid valve. The skewer was ingested approximately four months prior to referral and was partially removed via gastrotomy. The presenting complaint at the time of referral included coagulopathy, anemia and leukocytosis. A linear, hyperechoic structure was identified in the right heart during an echocardiogram. The foreign body was suspected to be a portion of the skewer that the patient had previously ingested. Cardiopulmonary bypass was performed and the foreign body was removed successfully. Complications following surgery included the development of tricuspid valve and ventricular wall thrombi, atrial flutter and amiodarone toxicity. Many indications have been described in the veterinary literature for cardiopulmonary bypass. However, to the best of the authors' knowledge, successful removal of an intracardiac foreign body with cardiopulmonary bypass has not been reported in a veterinary patient. This represents a new indication for cardiopulmonary bypass in veterinary medicine. 相似文献
100.
A study of the progress of the Aujeszky's disease control programme in Italy using survival analysis
Martini M Drigo M Dalla Pozza M Ferrari G Martinello F Sona B 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2003,50(4):191-195
A 3-year study (1997-2000) was performed on 294 swine herds from Italy, where a National Programme of Control of Aujeszky's Disease (AD) based on compulsory vaccination has been operative since 1997. Aim of the study was to evaluate the progress of this control programme using a survival approach applied to gE-seropositive herds at the beginning of the programme. The cumulative proportion of herds still gE-seropositive at the end of the study was 0.57. No significant difference in the probability of becoming gE-seronegative during the study period was found between herds of different type (breeding versus farrow-to-finish) whereas significant differences were seen between herds from different areas. The Cox's proportional hazards regression, performed on data from 79 herds, showed that the only risk factor significantly associated with a higher probability of becoming gE-seronegative is again the geographical location. Other risk factors considered in the analysis were: type of enterprise, type of replacement of animals, herd size, pig and pig herds densities around the farm, distance from the nearest pig herd and year of beginning of the vaccination with a gE-deleted vaccine. 相似文献