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131.
Role of NMDA receptor subtypes in governing the direction of hippocampal synaptic plasticity 总被引:1,自引:0,他引:1
Liu L Wong TP Pozza MF Lingenhoehl K Wang Y Sheng M Auberson YP Wang YT 《Science (New York, N.Y.)》2004,304(5673):1021-1024
Activation of N-methyl-d-aspartate subtype glutamate receptors (NMDARs) is required for long-term potentiation (LTP) and long-term depression (LTD) of excitatory synaptic transmission at hippocampal CA1 synapses, the proposed cellular substrates of learning and memory. However, little is known about how activation of NMDARs leads to these two opposing forms of synaptic plasticity. Using hippocampal slice preparations, we showed that selectively blocking NMDARs that contain the NR2B subunit abolishes the induction of LTD but not LTP. In contrast, preferential inhibition of NR2A-containing NMDARs prevents the induction of LTP without affecting LTD production. These results demonstrate that distinct NMDAR subunits are critical factors that determine the polarity of synaptic plasticity. 相似文献
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David Holt BVSc DACVS Sunil Singhal MD Laura E. Selmic BVetMed MPH DACVS 《Veterinary surgery : VS》2020,49(1):33-43
Surgical excision is the foundation of treatment for early-stage solid tumors in man and companion animals. Complete excision with appropriate margins of surrounding tumor-free tissue is crucial to survival. Intraoperative imaging allows real-time visualization of tumors, assessment of surgical margins, and, potentially, lymph nodes and satellite metastatic lesions, allowing surgeons to perform complete tumor resections while sparing surrounding vital anatomic structures. This Review will focus on the use of near-infrared imaging and optical coherence tomography for intraoperative tumor visualization. 相似文献
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Ravindra M. Aurade S.MD. Akbar Hanumanth GoudSenigala K. Jayalakshmi Kuruba Sreeramulu 《Pesticide biochemistry and physiology》2011,101(3):212-219
Flavonoids (morin, quercetin and phloroglucinol) were tested for their ability to modulate the function of P-glycoprotein ATPase of the insecticide resistant pest Helicoverpa armigera (Ha-Pgp). Flavonoids in the presence of ethylparaoxon or cypermethrin significantly reduced both larval weight as well as survival rate 40-50%. Morin and quercetin inhibited the activity of Ha-Pgp ATPase by 80-90%, whereas phloroglucinol inhibited ATPase activity by 40% at 100 μM concentration. These flavonoids inhibited the verapamil, ethylparaoxon and cypermethrin-stimulated Ha-Pgp ATPase activity. Morin, quercetin and phloroglucinol binding were quantitated by quenching of the intrinsic Trp fluorescence of purified Ha-Pgp ATPase. Drug transport was monitored in proteoliposomes containing Ha-Pgp ATPase using the high affinity fluorescent substrate tetramethylrosamine (TMR) in real time. Addition of the morin and quercetin mediated the collapse of the TMR concentration gradient generated by Ha-Pgp ATPase. The inhibition studies on Ha-Pgp ATPase activity may contribute towards understanding new strategies of the pest to overcome insecticide resistance. 相似文献
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JOHN R. FIKE PHD CHRISTOPHER E. CANN PHD KRZYSZTOF TUROWSKI MD ROBERT J. HIGGINS BSVC PHD JANE M. TURRELL DVM LIV AX RT LIV AX RT 《Veterinary radiology & ultrasound》1986,27(4):121-128
The uptake and washout of iodinated contrast medium in neoplastic and non-neoplastic lesions in the dog brin was quantified using computed tomography. The magnitude of tissue contrast enhancement was measured during and up to 15 minutes following an intravenous infusion of contrast medium. Compartmental analysis was used to obtain rate contants for contrast medium movement into and out of brain lesions. In non-glioma tumors, contrast enhancement was maximum during the infusion and the rate of contrast washout was comparable to that measured in the normal brain. Mixed gliomas also showed maximum enhancement during infusion, but there was no washout of contrast. Contrast enhancement in radiation-induced brain damage and cystic encephalomalacia secondary to tumor compression continued to increase for 5–15 minutes after infusion; rate constants in these non-tumor lesions were different from all tumors studied. Hyperthermia-induced lesions had comparable rate constants for contrast washin and washout. These results indicate that kinetic CT studies provide a non-invasive measure of permeability differences between lesions, may be useful in differentiating certain types of intracranial lesions, and may provide an effective method for patient follow-up after treatment. 相似文献