排序方式: 共有44条查询结果,搜索用时 15 毫秒
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Ignacio Velázquez Silvia Valencia Alberto López-Lera Alicia de la Peña Milagros Candela 《Euphytica》2004,137(1):73-79
We have evaluated the regeneration ability of leaf and root explants in 26 natural populations with the aim of analyzing the
naturally occurring variation of Arabidopsis in vitro shoot and root organogenesis. All the accessions have been used as parental lines of available or under construction recombinant
inbred lines (RILs) under construction. Phenotypic differences found between Ler and Cvi ecotypes were mapped in a population of 162 RILs. Several involved quantitative trait loci (QTL) have been identified.
The analysis of near isogenic lines (NILs) would allow us to assign the effect of the QTLs identified and also possible candidate
genes will be tested among those already described in the literature mapping into those regions.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
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A novel approach for the biological control of invasive Bagrada bugs with entomopathogenic nematodes
Lankin Gabriela Santiagos Alejandra Hermosilla Milagros Aballay Erwin San-Blas Ernesto 《Journal of pest science》2022,95(2):699-707
Journal of Pest Science - The Bagrada bug, Bagrada hilaris Burmeister (Hemiptera: Pentatomidae), an invasive pest of Palearctic origin, has become a problem in the Western Hemisphere, attacking... 相似文献
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Paola Ceroli Luz Milagros Garcia Procaccini Graciela Corbino Maria Cristina Monti Marcelo Huarte 《Potato Research》2018,61(3):219-229
Potato cubes of cultivar Innovator were subjected to different food conservation treatments: osmotic dehydration and antioxidants (OD + A), edible coating (E), edible coating with osmotic dehydration and antioxidants (EC + OD + A), immersion in antioxidants (A) and microwave blanching (B). The quality of the products was evaluated by measuring dry matter content, colour, browning index, total phenols and antioxidant capacity and polyphenol oxidase (PPO) activity. Treatments with A resulted in a lower a* colour parameter (red to green) but increased total phenols and antioxidant activity. Those treatments with OD and A together resulted in even larger increases in total phenols and antioxidant activity and also reduced PPO to negligible activity. OD increased dry matter content in OD + A treatments. B increased the browning index (BI) and colour parameters a* and ΔE (total change). As a consequence, potato cubes treated with osmotic dehydration and antioxidants had improved quality characteristics and these treatments are appropriate for minimally processed vegetables. 相似文献
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Protease inhibitor activity is associated to a basic chitinase from potato but not to an acidic one 总被引:1,自引:0,他引:1
Milagros Machinandiarena Marta Castillo Florencia Olivieri Gustavo Daleo Claudia Oliva 《Potato Research》2001,44(2):187-195
Summary Several pathogenesis-related proteins, which are produced in plants submitted to stress, have been identified as chitinases.
We have previously described that a potato basic chitinase strongly inhibited the activity of an aspartic protease isolated
from the same source. In this work, we have tested the activity of two potato chitinases as protease inhibitors. A basic chitinase
(ChiB) inhibited trypsin, chymotrypsin, subtilisin, proteinase K and a serine protease ofFusarium sp. An acidic one (ChiA) did not show inhibitory activity. The kinetics of trypsin inhibition by ChiB revealed different
patterns of inhibition with azocasein and BAPNA. Metal ions affected differentially both activities, suggesting that ChiB
is a bifunctional protein. These results and those reported by other authors suggest a new physiological role for pathogenesis-related
proteins. However, results presented in this paper suggest that the protease inhibitor activity is not a general characteristic
of potato chitinases. 相似文献
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Alberto J. Martín-Rodríguez Fernando Reyes Jesús Martín Juan Pérez-Yépez Milagros León-Barrios Alan Couttolenc César Espinoza ángel Trigos Víctor S. Martín Manuel Norte José J. Fernández 《Marine drugs》2014,12(11):5503-5526
In our search for quorum-sensing (QS) disrupting molecules, 75 fungal isolates were recovered from reef organisms (endophytes), saline lakes and mangrove rhizosphere. Their QS inhibitory activity was evaluated in Chromobacterium violaceum CVO26. Four strains of endophytic fungi stood out for their potent activity at concentrations from 500 to 50 μg mL−1. The molecular characterization, based on the internal transcribed spacer (ITS) region sequences (ITS1, 5.8S and ITS2) between the rRNA of 18S and 28S, identified these strains as belonging to four genera: Sarocladium (LAEE06), Fusarium (LAEE13), Epicoccum (LAEE14), and Khuskia (LAEE21). Interestingly, three came from coral species and two of them came from the same organism, the coral Diploria strigosa. Metabolic profiles obtained by Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS) suggest that a combination of fungal secondary metabolites and fatty acids could be the responsible for the observed activities. The LC-HRMS analysis also revealed the presence of potentially new secondary metabolites. This is, to the best of our knowledge, the first report of QS inhibition by marine endophytic fungi. 相似文献
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Jose V.?TarazonaEmail author Maria D.?Fernandez M. Milagros?Vega 《Journal of Soils and Sediments》2005,5(2):121-124
Background, Aims and Scope This paper presents the new Spanish regulation on contaminated soils and its scientific basis. The regulation offers a regulatory framework for establishing industrial activities which may result in soil contamination, and presents the methodology for setting the generic reference levels of organic pollutants.Legal Framework The Spanish regulation on contaminated soils is derived from the waste legislation and covers 101 industrial activities, as well as facilities handling significant amounts of hazardous chemicals (over 10,000 kg per year) or fuel (300,000 l fuel per year or storing 50,000 l fuel at any time). The regulatory framework includes initial declarations and a tiered system for selecting those soils requiring a proper site-specific risk assessment.Scientific Basis The regulation is risk based, and covers human and environmental risks. The human health risk assessment focuses on chemical analysis; the selection of relevant exposure routes is associated to the soil uses. The environmental risk assessment includes chemical analysis and direct toxicity testing, and covers three main ecological receptors: Soil organisms, associated aquatic systems and terrestrial vertebrates. Low-risk threshold concentrations are established as generic reference levels; if exceeded, a site-specific risk assessment is required. The detection of a very high level of acute toxicity of soil or leachates led to the declaration of the soil as contaminated due to the capacity for contaminating the adjacent areas.Conclusion Overall, the Spanish regulation offers a balance for combining regulatory needs, proper scientific basis and practicability. The use of European risk assessment protocols and the European legal framework would facilitate the pan-European extrapolation of this approach. The inclusion of direct toxicity testing as a legal method for classifying a soil as contaminated is considered a key element. 相似文献
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M. Pilar Cordovilla Milagros Bueno Carolina Aparicio Miguel Urrestarazu 《Journal of plant nutrition》2014,37(6):875-888
This study evaluated the effects of salinity on thyme (Thymus vulgaris) and lavender (Lavandula angustifolia) plants grown alone and in combination with each other. After transplanting, two-month-old plants received nutrient solutions supplemented with 0, 50, and 100 mM sodium chloride (NaCl) for 21 d. Thyme and lavender grown alone were each more tolerant to salt stress than thyme and lavender grown together. In the 100 mM NaCl treatment, all lavender plants grown with thyme died. In thyme, the carbon (C) and nitrogen (N) contents of the roots increased. Ethylene production in thyme was stimulated by salinity only in plants that interacted with lavender. However, in lavender, ethylene production was not influenced by the presence of thyme. The production of essential oils (EOs) was increased by salinity in thyme plants, whereas the EO production of lavender plants depended on the presence of thyme. 相似文献
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Stacy BA Wellehan JF Foley AM Coberley SS Herbst LH Manire CA Garner MM Brookins MD Childress AL Jacobson ER 《Veterinary microbiology》2008,126(1-3):63-73
Herpesviruses are associated with lung-eye-trachea disease and gray patch disease in maricultured green turtles (Chelonia mydas) and with fibropapillomatosis in wild sea turtles of several species. With the exception fibropapillomatosis, no other diseases of wild sea turtles of any species have been associated with herpesviral infection. In the present study, six necropsied Atlantic loggerhead sea turtles (Caretta caretta) had gross and histological evidence of viral infection, including oral, respiratory, cutaneous, and genital lesions characterized by necrosis, ulceration, syncytial cell formation, and intranuclear inclusion bodies. Nested polymerase chain reaction targeting a conserved region of the herpesvirus DNA-dependent-DNA polymerase gene yielded two unique herpesviral sequences referred to as loggerhead genital-respiratory herpesvirus and loggerhead orocutaneous herpesvirus. Phylogenetic analyses indicate that these viruses are related to and are monophyletic with other chelonian herpesviruses within the subfamily alpha-herpesvirinae. We propose the genus Chelonivirus for this monophyletic group of chelonian herpesviruses. 相似文献