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11.
Debra L Miller Sreekumari Rajeev Milagros Brookins Jeff Cook Lisa Whittington Charles A Baldwin 《Journal of zoo and wildlife medicine》2008,39(3):445-449
Four species (Dendrobates auratus, Phyllobates terribilis, Pyxicephalus adspersus, and Rhacophorus dennysi) of captive anurans with a clinical history of lethargy and inappetence were found dead and were submitted for necropsy. Gross lesions included irregular patches of sloughed skin and rare dermal ulcerations. Histologic findings included epidermal proliferation that was most pronounced on the digits and that included intracytoplasmic chytrid organisms. Bacteria were often associated with the epidermal lesions. Intracytoplasmic inclusion bodies were observed in hepatocytes. Real-time polymerase chain reaction yielded positive results for both Ranavirus and Batrachochytrium dendrobatidis (Bd). Bacterial culture of internal organs yielded Aeromonas hydrophila. This is the first report of concurrent infections in anurans by Ranavirus and Bd and A. hydrophila. 相似文献
12.
Rosario Martínez Alejandro García-Beltrn Garyfallia Kapravelou Cristina Mesas Laura Cabeza Gloria Perazzoli Palmira Guarnizo Alberto Rodríguez-Lpez Roberto Andrs Vallejo Milagros Galisteo Pilar Aranda Jose Prados María Lpez-Jurado Consolacin Melguizo Jesus M. Porres 《Marine drugs》2022,20(5)
Nannochloropsis gaditana is a microalga with interesting nutritional and functional value due to its high content of protein, polyunsaturated fatty acids, and bioactive compounds. However, the hardness of its cell wall prevents accessibility to these components. This work aimed to study the effect of a treatment to increase the fragility of the cell wall on the bioavailability of its nutrients and functional compounds. The antioxidant and antiproliferative capacity of functional extracts from treated and untreated N. gaditana was assessed, and the profile of bioactive compounds was characterized. Furthermore, to study the effect of treatment on its nutrient availability and functional capacity, an in vivo experiment was carried out using a rat experimental model and a 20% dietary inclusion level of microalgae. Functional extracts from treated N. gaditana exhibited higher antioxidant activity than the untreated control. Furthermore, the treated microalga induced hypoglycemic action, higher nitrogen digestibility, and increased hepatic antioxidant activity. In conclusion, N. gaditana has interesting hepatoprotective, antioxidant, and anti-inflammatory potential, thus proving itself an ideal functional food candidate, especially if the microalga is treated to increase the fragility of its cell wall before consumption. 相似文献
13.
Gilbert-López B García-Reyes JF Mezcua M Molina-Díaz A Fernández-Alba AR 《Journal of agricultural and food chemistry》2007,55(26):10548-10556
A multiresidue method using liquid chromatography-time-of-flight mass spectrometry (LC-TOFMS) has been developed for the quantitative analysis of five widely used postharvest fungicides (carbendazim, thiabendazole, imazalil, prochloraz, and iprodione) and two of their transformation products (imazalil and prochloraz metabolites) in fruit juices. LC-TOFMS in positive electrospray ionization mode was used to quantify and confirm trace levels of these fungicides in fruit juices. The proposed method consists of a sample treatment step based on solid-phase extraction using hydrophilic-lipophilic-balanced polymer-based reverse-phase SPE cartridges (Oasis HLB) and methanol as an eluting solvent. Fruit-juice extracts spiked at different fortification levels (10 and 20 microg L(-1)) yielded average recoveries in the range of 71-109% with RSD (%) below 15%. Subsequent identification, confirmation, and quantitation were carried out by LC-TOFMS analysis. The confirmation of the target species was based on accurate mass measurements of protonated molecules ([M+H]+) and fragment ions, obtaining routine accuracy errors lower than 2 ppm in most cases. The obtained limits of detection (LODs) of the proposed method were in the range of 0.08-0.45 microg L(-1). Finally, the proposed method was successfully applied to the analysis of 23 fruit juice samples collected from different European countries and the United States, showing the potential applicability of the method in routine analysis. Over 50% of the samples tested contained pesticide residues, but relatively low concentration levels were found. 相似文献
14.
M. Isabel García Mercedes Lozano Vicente Montero de Espinosa M. Concepción Ayuso M. Josefa Bernalte M. Carmen Vidal-Aragón M. Milagros Pérez 《Scientia Horticulturae》2007
The production of sweet paprika in Spain uses exclusively fruit of Bola-type Capsicum annuum L. This work describes the evaluation of the agronomic behaviour of five new cultivars of the Bola-type paprika red pepper, selected in the Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), and grown in Extremadura for 3 years. The colour and the pigment content of the paprika elaborated following the traditional procedure of La Vera were also studied. 相似文献
15.
Eleonor A. Tendencia Milagros R. dela Pea Casiano H. Choresca Jr. 《Aquaculture (Amsterdam, Netherlands)》2006,260(1-4):54-60
The antibacterial effect of the presence of Tilapia hornorum against luminous bacteria in shrimp culture has been reported. This study investigates how the presence of commercially valued marine species such as seabass, snapper and siganid affect the growth of luminous bacteria in shrimp culture water. Results showed that luminous bacterial count of water stocked with seabass, siganid and snapper are significantly lower than those without fish. Therefore this study has demonstrated that seabass, siganid and snapper are alternative species for culture with shrimp to control or inhibit the growth of luminous bacteria in shrimp ponds. 相似文献
16.
Piccinelli AL García Mesa M Armenteros DM Alfonso MA Arevalo AC Campone L Rastrelli L 《Journal of agricultural and food chemistry》2008,56(5):1574-1581
A hydroalcoholic extract of lime ( Citrus aurantifolia) leaves has been developed in Cuba to be used as a nutritional supplement and phytomedicine in the form of tincture (TLL). A HPLC-PDA-ESI/MS/MS method has been used for the comprehensive analysis of C-glycosyl flavones in TLL. Six C-glycosyl flavones were characterized and, to confirm the proposed structures and to elucidate the nature of the sugar units, a preparative procedure was applied, and isolated compounds were characterized by NMR. Apigenin-6,8-di-C-beta-D-glucopyranoside (vicenin II) (1), diosmetin-6,8-di- C-beta- d-glucopyranoside (2), apigenin-8-C-beta-D-glucopyranoside (vitexin) (3), apigenin-8-C-[alpha-L-arabinopyranosyl-(1-->6)]-O-beta-D-glucopyranoside (4), apigenin-6-C-[alpha-l-arabinopyranosyl-(1-->6)]-O-beta-D-glucopyranoside (5). and apigenin-6-C-beta-D-glucopyranoside (isovitexin) (6) were identified in TLL and quantified by HPLC-PDA. Compounds 4 and 5 were two new arabinosyl derivatives of vitexin and isovitexin. Inhibitor effect of TLL on platelet aggregation induced by physiological agonists of platelets was evaluated in human plasma. TLL inhibited significantly ADP and epinephrine-induced platelet aggregation in a concentration-dependent manner (IC 50=0.40 and 0.32 mg/mL, respectively). 相似文献
17.
Arsenic and Heavy Metal Uptake and Accumulation in Native Plant Species from Soils Polluted by Mining Activities 总被引:1,自引:0,他引:1
Sara García-Salgado David García-Casillas Ma. Angeles Quijano-Nieto Ma. Milagros Bonilla-Simón 《Water, air, and soil pollution》2012,223(2):559-572
Arsenic and heavy metal (specifically Cd, Cr, Cu, Ni, Pb, and Zn) uptake, translocation, and accumulation in ten native plant
species spontaneously growing in soils polluted by mining activities were studied, with a focus on future phytoremediation
work in polluted soils. Plant and soil samples were collected in the vicinity of the Mónica mine (NW Madrid, Spain). Soil
analysis showed the ability of native plants for growing in soils with high concentration levels of Cd, Cu, Pb, Zn, and especially
As. From these elements, the highest percentage of extractable elements was found for Cd and the lowest for Pb. A highly significant
correlation was observed between total and extractable element concentrations in soils, except for Cu, indicating that total
concentration is the most relevant factor for element mobility in these soils. Extractable elements in soils were better correlated
with concentrations in plants than total elements in soils; thus, extraction methods applied are suitable to estimate the
element phytoavailable fraction in soils, which depends on the plant species and not only on the element mobility in soils.
High element concentrations were found in the aboveground parts of Corrigiola telephiifolia (As and Pb), Jasione montana (Cd and Zn), and Digitalis thapsi (As, Cd, Cu, Pb and Zn). However, considering the translocation and accumulation factors, together with the concentration
levels found in roots and aboveground parts, only C. telephiifolia could be considered a Pb accumulator and an As hyperaccumulator plant, which could be used for future phytoremediation work
in soils polluted with As. 相似文献
18.
Levan AJ Tanvir NR Cenko SB Perley DA Wiersema K Bloom JS Fruchter AS Postigo Ade U O'Brien PT Butler N van der Horst AJ Leloudas G Morgan AN Misra K Bower GC Farihi J Tunnicliffe RL Modjaz M Silverman JM Hjorth J Thöne C Cucchiara A Cerón JM Castro-Tirado AJ Arnold JA Bremer M Brodie JP Carroll T Cooper MC Curran PA Cutri RM Ehle J Forbes D Fynbo J Gorosabel J Graham J Hoffman DI Guziy S Jakobsson P Kamble A Kerr T Kasliwal MM Kouveliotou C Kocevski D Law NM Nugent PE Ofek EO Poznanski D 《Science (New York, N.Y.)》2011,333(6039):199-202
Variable x-ray and γ-ray emission is characteristic of the most extreme physical processes in the universe. We present multiwavelength observations of a unique γ-ray-selected transient detected by the Swift satellite, accompanied by bright emission across the electromagnetic spectrum, and whose properties are unlike any previously observed source. We pinpoint the event to the center of a small, star-forming galaxy at redshift z = 0.3534. Its high-energy emission has lasted much longer than any γ-ray burst, whereas its peak luminosity was ~100 times higher than bright active galactic nuclei. The association of the outburst with the center of its host galaxy suggests that this phenomenon has its origin in a rare mechanism involving the massive black hole in the nucleus of that galaxy. 相似文献
19.
20.
Medina-Pastor P Valverde A Pihlstrom T Masselter S Gamon M Mezcua M Rodriguez-Torreblanca C Fernandez-Alba AR 《Journal of agricultural and food chemistry》2011,59(14):7609-7619
Practical "top-down" approaches appear to be the most suitable for the evaluation of measurement uncertainty in pesticide residue testing laboratories, where analytical procedures are routinely applied to a large number of pesticide/food combinations. The opposite approach, "bottom-up" evaluation of measurement uncertainty, leads to great difficulties in evaluating all of the pesticides in a consistent way. Among the top-down approaches, there are two main ways in which measurement uncertainty can be estimated: One is based on default values, which are based on previous extensive interlaboratory experience and the proven accuracy of the laboratory; these include the Horwitz equation or the fit-for-purpose relative standard deviation (FFP-RSD). The other is based on experimental data from the quality control work of the laboratory: within-laboratory reproducibility, interlaboratory validation, or a combination of results obtained in proficiency tests. The principal existing guidelines from various bodies (Eurachem, Nordtest, and Eurolab) all propose different approaches for calculating measurement uncertainty. In this paper, the main top-down approaches are evaluated and compared using the data from the European Proficiency Test Database for Fruits and Vegetables and the Multiresidue Method validation databases obtained from the National Reference and Official Laboratories in Europe. The main conclusion of the comparative study is that a default expanded measurement uncertainty value of 50% could satisfy all of the requirements for facilitating and harmonizing, worldwide, the intercomparability of the pesticide residue confidence results between laboratories. 相似文献