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31.
In many countries, test-and-slaughter policies based on tuberculin skin testing have made a significant impact on the control of bovine tuberculosis (caused by infection with Mycobacterium bovis). However, in some countries these policies have not proved as effective and improved disease control strategies are required (including improved diagnostic tests and development of vaccines). The host pathogen interactions in bovine tuberculosis are very complex. While studies of the disease in naturally infected field cases of bovine tuberculosis have provided valuable information, detailed knowledge can also be gained through studies of disease models. A number of studies have developed M. bovis infection models employing a range of routes and challenge doses. An early objective was assessment of vaccine efficiency, and models of infection remain central to current work in this area. Development of the intra-nasal and intra-tracheal models have also advanced our understanding of the kinetics of the immune response. In many of these studies, understanding of pathogenesis has been improved by definition of the cells that respond to infection and those that are instrumental in modulation of host responses. Experimental models of infection have been adapted to study cattle to cattle transmission, modeling one of the fundamental routes of infection. This review provides a historical perspective on the types of experimental models used in over 100 years of research and outlines new opportunities to refine those methods for bovine and human tuberculosis and to contribute to improved diagnostics, advanced understanding of immunology and vaccine design. 相似文献
32.
M.L. Pollock J.P. Holland C. Morgan-Davies J. Morgan-Davies A. Waterhouse 《Strength and Conditioning Journal》2013,66(4):387-400
Traditional upland livestock grazing is declining worldwide, leading to concerns about possible impacts on biodiversity. Although monitoring of protected areas often focuses on rare species, management changes also affect currently common species and vegetation structure. “Biodiversity” is a concept rather than a simple variable, and monitoring it requires indicators that are widely applicable and appropriate to context. This paper presents a novel knowledge-driven approach to developing a selection of biodiversity indicators that can then be rapidly and objectively measured within rangeland environments. Stakeholder and professional opinion on likely changes in biodiversity following reductions in sheep grazing was elicited using a workshop-based process. Potential variables suitable for use as biodiversity indicators were developed and professional opinion on their usefulness sought. A number of indicators were then tested in a natural experiment field study on the impact of reduced sheep grazing in Scotland. In the field study, red deer abundance appeared to increase where sheep grazing was reduced. It was therefore necessary to use estimates of both sheep and deer abundance as explanatory variables. In agreement with the professionals’ predictions, dwarf shrub abundance and vegetation height were greater where sheep grazing had been reduced, after taking into account differences in deer. In contrast to the professionals’ predictions, the field results showed rough grasses and dead material were less abundant where sheep had been reduced, with deer also having an impact on dead material. The professionals were unsure of the effects of reduced sheep grazing on vegetation mosaic structure; the field results suggested that reduced sheep grazing leads to a reduction in structural heterogeneity, but that deer had the greater impact in this case. Several other predictions showed nonsignificant differences in relation to reduced sheep grazing. The participatory method and some of the field methods presented are cost effective and widely applicable. 相似文献
33.
H. J. Cottle K. J. Hughes A. W. Philbey P. J. Pollock 《Equine Veterinary Education》2008,20(11):575-579
An 11‐year‐old Clydesdale gelding was presented for investigation of left forelimb lameness of 2 weeks' duration. The use of scintigraphic imaging helped to localise the source of lameness to the left proximal humerus. In this report, the clinical and diagnostic imaging features of a primary osseous haemangiosarcoma in a horse are described, along with the challenges of establishing a definitive diagnosis ante mortem. In addition, neoplasia of the appendicular skeleton should be considered a differential cause of lameness in the horse. 相似文献
34.
35.
This report describes a four-day-old, full-term Connemara colt, presented for the evaluation of a progressive inability to rise unassisted. A diagnosis of nutritional muscular dystrophy was made based on muscular weakness, elevated muscle enzymes and low vitamin E, selenium and glutathione peroxidase activity. The foal was treated with intramuscular vitamin E-selenium and made a full recovery. 相似文献
36.
Soil moisture and water use by pastures and silvopastures in a sub-humid temperate climate in New Zealand 总被引:1,自引:1,他引:0
Soil moisture content from 0 to 2 m depth was monitored under 2–6 year old radiata pine (Pinus radiata) with three understoreys of bare ground, lucerne (Medicago sativa) and ryegrass/clover (Lolium perenne/Trifolium spp.) and under adjacent open-grown lucerne and ryegrass/clover pastures. By the fifth year soil moisture depletion/recharge
pattern under the trees alone was similar to that under open pasture and under trees with pasture understoreys. Maximum plant
available moisture storage was 207–223 mm in the top meter of this Templeton silt loam soil but only 69–104 mm at 1–2 m depth
where coarse textures often predominated. Lucerne reduced soil moisture content (SMC) to lower levels during drier summers
and extracted more water from 1 to 2 m depth than ryegrass/clover. Evapotranspiration (ET) during early summer when soil moisture
was high was close to the Penman potential evapotranspiration (E
p
), but the difference increased when SMC in the top meter dropped below 200 mm. The silvopasture treatments had higher ET
in winter than pasture alone but this was still less than E
p
. Soil moisture deficits (SMD) at the end of each summer were sufficiently large to require slightly higher than normal winter
rainfall and ET < E
p
to recharge the soil to field capacity before the next summer. The soil moisture results, taken together with root and growth
data, suggest that trees and understorey pastures are complementary in the first three or four growing seasons but this balance
subsequently declines in favor of the pine trees. Management options, to extend the period that understorey pastures are productive,
include reducing tree stockings, more vigorous pruning, using competitive understoreys and changing from pines to deciduous
trees. Research on new silvopastoral combinations is suggested. 相似文献
37.
R J Pollock M S Pollock M C O Ferrari S G W Kaminskyj D P Chivers 《Journal of fish diseases》2012,35(4):249-254
Fish in the Superorder Ostariophysi possess large epidermal club cells that release chemical cues warning nearby conspecifics of danger. Despite the long‐held assumption that such club cells evolved under the selective force of predation, recent studies demonstrated that predation has no effect on club cell investment. Rather, club cells have an immune function and cell production may be stimulated by skin‐penetrating pathogens and parasites. The current work investigates whether fathead minnows, Pimephales promelas, alter their club cell characteristics based on variation in infection risk. In a 2 × 3 design, we exposed minnows to infective cysts of two oomycete species (Saprolegnia ferax and S. parasitica) at three different concentrations (2, 20 or 200 cysts L?1). Club cell characteristics (number and size) were quantified 12 days after exposure. Saprolegnia parasitica is thought to be more pathogenic than S. ferax, hence we predicted greater club cell investment and a larger turnover rate of cells by minnows exposed to S. parasitica than S. ferax. We also predicted that minnows exposed to higher numbers of cysts should invest more in club cells and have a higher turnover rate of cells. We found no difference in club cell density or size between fish exposed to the two Saprolegnia species; however, fish exposed to high concentrations of pathogens had smaller club cells than those exposed to low concentrations, indicating a higher rate of turnover of cells in the epidermis. 相似文献
38.
39.
Jason R. Burgess-Conforti Kristofor R. Brye David M. Miller Erik D. Pollock Lisa S. Wood 《Water, air, and soil pollution》2018,229(2):34
Due to low cost and large abundance, coal continues to be one of the major energy sources for electricity generation in the USA. The dry desulfurization of flue gases during coal combustion produces a by-product that may be potentially useful as a soil amendment. However, the influence of dry flue gas desulfurization (DFGD) by-products on trace element losses with runoff from treated fields has not been well investigated. The objective of this study was to evaluate the effects of land application of a high-Ca, DFGD by-product on trace elements lost in runoff from natural rainfall events. The by-product was applied once on May 18, 2015 at 9 Mg DFGD ha?1 to small plots of a highly weathered Ultisol under managed-grassland land use in northwest Arkansas. Runoff was collected following each runoff-producing precipitation event for 1 year. Seasonal (i.e., summer (May to August), fall (August to November), winter (November to February), and spring (February to May)) runoff, annual runoff, runoff pH, and electrical conductivity did not differ significantly between DFGD treatments. Seasonal flow-weighted mean Ni concentrations and seasonal V loads were significantly greater in runoff when amended versus the unamended control when compared during at least one 3-month season by 44.5 and 86.9% for Ni and V, respectively. Based on the results of this study, it appears that land application of a high-Ca DFGD by-product at rates ≤?9 Mg ha?1 has minimal effects on trace elements in runoff. 相似文献