Variability of certain chemotypes in three accessions of German chamomile (Matricaria chamomilla L.)

In 203 analyzed chamomile plants, several compounds appreciated for their medicinal value, were evaluated qualitatively and quantitatively. α-Bisabolol and its oxides A and B were measured by gas chromatography; matricine as a precursor of chamazulene, was assessed semi-quantitatively by matching visually thin layer chromatography bands with a concentration range of the pure substance and the flavonoids apigenin and apigenin-7-glycoside were quantified by high performance liquid chromatography. Mean values of all compounds were compared in the three chamomile accessions ‘Manzana’, ‘Lutea’ and ‘Hungarian 2’. Correlations between different substances over all data were calculated. Moreover, certain groups combining particular properties according to their desired purpose or specificity, were formed. Their incidence in the particular chamomile varieties was determined. ‘Manzana’, a tetraploid cultivar, exhibited the highest share of the valuable bisabolol–matricine-type and the lowest ratio of bisabolol-oxides to bisabolol. To develop ‘high-quality-populations’, defined individuals of this cultivar shall be selected for further breeding.     

Evolutionary formation of new centromeres in macaque

A systematic fluorescence in situ hybridization comparison of macaque and human synteny organization disclosed five additional macaque evolutionary new centromeres (ENCs) for a total of nine ENCs. To understand the dynamics of ENC formation and progression, we compared the ENC of macaque chromosome 4 with the human orthologous region, at 6q24.3, that conserves the ancestral genomic organization. A 250-kilobase segment was extensively duplicated around the macaque centromere. These duplications were strictly intrachromosomal. Our results suggest that novel centromeres may trigger only local duplication activity and that the absence of genes in the seeding region may have been important in ENC maintenance and progression.     

Effect of barley coffee on the adhesive properties of oral streptococci

Some beverages and foods protect tooth surfaces against Streptococcus mutans colonization. Adhesion of S. mutans is a crucial step in the initiation and development of dental caries. In this study, we showed that barley coffee (BC), a beverage made from roasted barley, interferes with S. mutans adsorption to hydroxyapatite (HA), and we identified its antiadhesive components. The effects of sublethal concentrations (sub-MICs) of BC on the adhesion of S. mutans to saliva-coated HA beads were assessed using three experimental approaches: (A) Beads were pretreated with BC before adding bacteria, (B) BC and bacteria were added to the beads simultaneously, and (C) streptococci grown in the presence of sub-MICs of BC were added to the beads. All treatments induced variable but significant inhibition of S. mutans sucrose-dependent and -independent adherence to HA. Similar results were obtained with other oral streptococci. BC components were fractioned by dialysis and gel filtration chromatography; the <1000 Da molecular mass (MM) fraction, which contains polyphenols, zinc, and fluoride ions, and the >1000 kDa MM fraction, which consists of a potent brown antioxidant, melanoidin, both displayed antiadhesive properties. High-MM melanoidin was not detected in unroasted barley, indicating that it forms during the roasting process. Results suggest that BC consumption may influence the colonization of tooth surfaces by cariogenic bacteria.     

Tears and aqueous humor from horses inoculated with Leptospira contain antibodies which bind to cornea

An antigenic relationship between Leptospira interrogans and equine cornea was previously described by us. An enzyme-linked immunosorbent assay was employed in the present work to investigate the existence of anti-leptospira and anti-cornea antibodies in tears, aqueous humor and serum from horses inoculated i.m. with those antigens. Ten days after a booster by the same route, antibodies that bind to microtiter plates, coated with an homogenate of either equine cornea or leptospira, were detected in those fluids and in the sera. At the same time, the corneas of the horses began to develop a diffuse opacity. This finding of anti-leptospira antibodies in equine tears and aqueous humor shows the pathway along which they arrive at the cornea and bind to it.