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排序方式: 共有525条查询结果,搜索用时 15 毫秒
141.
Jeryl C. Jones DVM PhD Peter K. Shires BVSc MS Karen D. Inzana DVM PhD D. Phillip Sponenberg DVM PhD Christiane Massicotte DVM MS Walter Renberg DVM MS Alain Giroux DVM 《Veterinary radiology & ultrasound》1999,40(2):108-114
The objective of this study was to evaluate intravenous contrast-enhanced computed tomography as a technique for predicting the within-level location(s) of compressive soft tissues in the canine lumbosacral spine. Pre-operative intravenous contrast-enhanced computed tomography of the L5-S3 vertebral levels was performed in 12 consecutive large breed dogs with lumbosacral stenosis. The images were evaluated for enhancement of soft tissues by two radiologists who were unaware of the surgical findings. For each within-level location (dorsal canal, ventral canal, right lateral recess, left lateral recess) enhancement was classified as present, absent or equivocal. The results were compared with the results of surgical exploration and histopathology of excised tissues. The positive predictive values of intravenous contrast-enhanced computed tomography for compressive soft tissues involving the dorsal canal, ventral canal and lateral recesses were 83%, 100%, and 81% respectively. Negative predictive values for compressive soft tissues involving these locations were 29%, 50%, and 40% respectively. 相似文献
142.
Victor S Panangala Craig A Shoemaker Phillip H Klesius Amitava Mitra & Riccardo Russo 《Aquaculture Research》2009,40(8):915-926
Cross-protection of channel catfish ( Ictalurus punctatus Rafinesque) immunized with a low dose of virulent Edwardsiella ictaluri and challenged with six E. ictaluri strains was examined in four trials. The relative per cent survival among low-dose immunized and then challenged fish ranged from 27.7% to 100%. Significant protection ( P <0.05), with the exception of strain ATCC-33202, was conferred by immunization with a given E. ictaluri strain challenged either with a homologous or a heterologous strain. Antibody titres of pooled serum collected on day 22 from surviving fish examined by enzyme-linked immunosorbent assay (ELISA) ranged from 1:40 to 1:320, but no differences were apparent among different vaccinated groups. The protein profiles of six E. ictaluri strains examined by Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) showed a relatively homogeneous pattern. Immuno-blots probed with pooled serum from immunized and challenged fish showed a pattern similar to LPS-reaction patterns observed with E. ictaluri in other studies. Since the present studies further corroborate that E. ictaluri is a clonal bacterial species with no apparent antigenic differences, it is possible that immunization with a single E. ictaluri field strain should confer protection against any other strain. 相似文献
143.
Ephraim Cohen Gary B. Quistad Phillip R. Jefferies John E. Casida 《Pest management science》1996,48(2):135-140
Several neem-seed extracts, some used for preparing commercial azadirachtin-containing insecticides, are cytotoxic to N1E-115 murine neuroblastoma cells with IC50 values of 20–200 μg extract ml−1 culture medium. Bioassay-directed fractionation by reversed-phase HPLC shows that the toxicity to N1E-115 cells is associated primarily with a single minor component identified by isolation and NMR and MS as nimbolide with an IC50 of 1·5 μg ml−1 (3·2 μM ). The difference in quantity of nimbolide in seven neem extract sources generally correlates with their overall cytotoxicity. Three other limonoids (epoxyazadiradione, salannin and possibly deacetylsalannin) but not azadirachtin, nimbin and deacetylnimbin contribute in small part to the cytotoxicity. Reconstituted neem extract with only nimbolide removed is less cytotoxic than the original extract. It therefore appears that nimbolide is the principal cytotoxic component of the neem extracts examined and that such minor constituents may warrant consideration in safety evaluations. 相似文献
144.
Policies for mitigating phosphorus (P) loss from agriculture are being developed in a number of European countries and calculation of P balance at farm‐gate or field‐scale is likely to be a part of such policies. The aim of the paper was to study P balance at the field‐scale in 18 countries that participated in the European Union's (EU) European Co‐operation in the Field of Scientific and Technical Research (COST) action on “Quantifying the Agricultural Contribution to Eutrophication (COST 832)”. A questionnaire related to P balance at the field‐scale was sent to representatives in the 18 countries and all replied. The field as a unit is defined differently in the various European agricultural systems. The identification of the inputs and outputs differ among the countries. For example, P losses may or may not be taken into account in balances. Phosphorus balance at the field‐scale is used in all countries in the context of soil analysis and P recommendations for crops and advisory and research purposes, while only a few countries use it for policy purposes (agri‐environmental). There is wide variation in P balances between countries in relation to soil fertility and vulnerability of water to eutrophication from nutrients from agricultural sources. In several eastern European countries, Hungary for example, fertilizer P use has dropped to about one tenth of the levels used in the 1980's. Many of these countries now have a negative P balance. In western European countries, by contrast, although fertilizer P use has decreased in recent years, the average input is higher than the average off take, and soil test phosphorus (STP) values remains high and continue to increase in some areas. Twelve different soil extractants for STP are used in Europe, and their interpretation can hinder direct comparisons. Calculating P balance at the field‐scale involves approximations in estimating inputs and outputs and spatial variations in fertility in individual fields. Accuracy of data and standardization of methods for calculating balances with inputs and outputs will be a challenge for the future development of a sustainable agriculture in Europe. 相似文献
145.
Shannon D. Boveland Phillip A. Moore Jagannatha Mysore Thomas M. Krunkosky Ursula M. Dietrich Carla Jarrett K. Paige Carmichael 《Veterinary ophthalmology》2010,13(2):81-90
Objective Determine the effects of matrix metalloproteinases (MMPs)‐2, ‐9, macrophage inflammatory protein‐2 (MIP‐2), tissue inhibitors of matrix metalloproteinase (TIMP)‐1 and ‐2 by immunohistochemical expression in fungal affected and purulonecrotic corneas. Procedure Paraffin‐embedded equine corneal samples; normal (n = 9), fungal affected (FA; n = 26), and purulonecrotic without fungi (PN; n = 41) were evaluated immunohistochemically for MMP‐2, ‐9, MIP‐2, TIMP‐1 and ‐2. The number of immunoreactive inflammatory cells was counted and statistics analyzed. Western blot was performed to detect MMP‐2, MMP‐9, TIMP‐1 and TIMP‐2 proteins. Results Matrix metalloproteinases‐2, ‐9, MIP‐2, TIMP‐1 and ‐2 immunoreactivity was identified in corneal epithelium of normal corneas, and in corneal epithelium, inflammatory cells, keratocytes, and vascular endothelial cells of both FA and PN samples. Inflammatory cell immunoreactivity was significantly higher in FA and PN samples than in the normal corneas. There was positive correlation between MMP‐2 and MIP‐2, MMP‐9 and MIP‐2, and MMP‐9 and TIMP‐1 in inflammatory cell immunoreactivity in FA samples. There was positive correlation between MMP‐9 and MIP‐2, MMP‐9 and TIMP‐2, MIP‐2 and TIMP‐1, and MIP‐2 and TIMP‐2 in inflammatory cell immunoreactivity in PN samples. Western blot confirmed the presence of all four proteins in equine corneal samples. Conclusion Increased immunoreactivity of MMP‐2 and ‐9 in FA and PN samples is indirectly related to MIP‐2 through its role in neutrophil chemo‐attraction. Tissue inhibitors of matrix metalloproteinase‐1 and TIMP‐2 are up‐regulated in equine purulonecrotic and fungal keratitis secondary to MMP‐2 and MMP‐9 expression. The correlation between MMPs ‐2 and ‐9, MIP‐2, TIMPs ‐1 and ‐2 suggests that these proteins play a specific role in the pathogenesis of equine fungal keratitis. 相似文献
146.
William Muir DVM PhD Diplomate ACVA Diplomate ACVECC Phillip Lerche DVM PhD Diplomate ACVA Ashley Wiese DVM MS Laura Nelson DVM Kirby Pasloske† DVM DVSc Diplomate ACVCP & Ted Whittem† BVSc PhD DACVCP 《Veterinary anaesthesia and analgesia》2009,36(1):42-54
ObjectiveTo determine the cardiorespiratory and anesthetic effects of 0, 5, 15, and 50 mg kg?1 intravenous (IV) alfaxalone in hydroxypropyl beta cyclodextrin (Alfaxan; Jurox Pty Ltd, Rutherford, NSW, Australia) in cats.Study designFour treatments of alfaxalone were administered in sequential order.AnimalsEight healthy adult cats (four male; four female) weighing between 3.71 and 5.91 kg.MethodsCats were instrumented for hemodynamic measurements. Four (0, 5, 15, and 50 mg kg?1) IV doses of alfaxalone were administered over one minute, with a 3-hour washout period between doses 0, 5, and 15 mg kg?1 on Day 0. The 50 mg kg?1 treatment was administered 24 hours later. Measurements of heart rate, aortic systolic, mean, and diastolic blood pressures, pulmonary arterial and right atrial mean pressures, cardiac output, respiratory rate, tidal and minute volumes, and arterial blood pH and blood gases (PaO2, PaCO2) were performed at pre-determined intervals. Systemic vascular resistance and rate pressure product were calculated. The quality of induction, maintenance, and recovery from anesthesia and the response to noxious stimulation were categorically scored.ResultsAlfaxalone administration resulted in dose-dependent cardiorespiratory depression. Decreases in arterial blood pressure and increases in heart rate occurred at higher doses. Most variables returned to baseline by 15-30 minutes. Respiratory rate, minute volume, and PaO2 decreased. Apnea was the most common side effect. Induction and maintenance quality were judged to be good to excellent at all doses and quality of recovery good to excellent at all but the 50 mg kg?1 dose. The duration of anesthesia and unresponsiveness to noxious stimulation increased with dose. The administration of the 50 mg kg?1 dose produced marked cardiorespiratory depression and apnea.Conclusions and clinical relevanceAlfaxalone produced dose-dependent anesthesia, cardiorespiratory depression and unresponsiveness to noxious stimulation in unpremedicated cats. Hypoventilation and apnea were the most common side effects. 相似文献
147.
J I Phillip 《Journal of the American Veterinary Medical Association》1992,200(3):302-304
148.
Virus pneumonia in calves 总被引:1,自引:0,他引:1
J I Phillip 《The Veterinary record》1970,86(10):280-284
149.
Graebing PW Chib JS Hubert TD Gingerich WH 《Journal of agricultural and food chemistry》2004,52(4):870-878
The photodegradation of [(14)C]niclosamide was studied in sterile, pH 5, 7, and 9 buffered aqueous solutions under artificial sunlight at 25.0 +/- 1.0 degrees C. Photolysis in pH 5 buffer is 4.3 times faster than in pH 9 buffer and 1.5 times faster than in pH 7 buffer. In the dark controls, niclosamide degraded only in the pH 5 buffer. After 360 h of continuous irradiation in pH 9 buffer, the chromatographic pattern of the degradates was the same regardless of which ring contained the radiolabel. An HPLC method was developed that confirmed these degradates to be carbon dioxide and two- and four-carbon aliphatic acids formed by cleavage of both aromatic rings. Carbon dioxide was the major degradate, comprising approximately 40% of the initial radioactivity in the 360 h samples from both labels. The other degradates formed were oxalic acid, maleic acid, glyoxylic acid, and glyoxal. In addition, in the chloronitroaniline-labeled irradiated test solution, 2-chloro-4-nitroaniline was observed and identified after 48 h of irradiation but was not detected thereafter. No other aromatic compounds were isolated or observed in either labeled test system. 相似文献
150.