Recent developments and improvements in ornamental fish packaging systems for air transport

Current ornamental fish packaging systems are characterized by very high fish loading densities and high metabolic wastes in the transport water after shipment. They focus mainly on management of the quality of transport water. Recent studies using the guppy as a model fish showed that post‐shipment mortality could be reduced through enhancement of the stress resistance of the fish, and hence emphases should also be placed on the preparation of the fish for transport and recovery of the fish after shipment. Farmers can contribute significantly by applying nutritional prophylaxis before harvesting. Exporters may use the salinity stress test to identify fish lots of good quality for transport, apply health prophylaxis to eradicate parasites and optimize other techniques such as starvation of the fish or addition of salt to the transport water to enhance the stress resistance of the fish. Importers may adopt proper acclimation procedure and allow fish to recover in low salinity water to reduce post‐shipment mortality. As the main bulk of post‐shipment mortality is stress‐mediated and occurs during the 1‐week recovery period, the industry should consider revising the basis of the current warranty system for their customers, from death on arrival to cumulative mortality at 7 days post shipment (or death after 7 days, DA7), in order to cut down fish losses after shipment.     

Decontamination of synthetic solutions containing heavy metals using chemically modified sawdusts bearing polyacrylic acid chains

New materials containing carboxylate groups have been synthesized by grafting polyacrylic acid onto sawdust. These new adsorbents were subjected to continuous extraction of different metal ions using packed columns to determine their maximum binding capacities. They exhibit binding capacities 15–40 times higher than unmodified sawdusts for removal of Cu²⁺, Ni²⁺, and Cd²⁺ chosen as representative heavy metal ions. Cation desorption and adsorbent regeneration could be effected by elution with a diluted HCl solution; water as an eluent has no effect on metal desorption.     

Kalmia removal increases nutrient supply and growth of black spruce seedlings: An effect fertilizer cannot emulate

In Canada's eastern boreal forest, the stagnant growth of black spruce (Picea mariana (Mill.) BSP) seedlings is often observed in the presence of ericaceous shrubs such as Kalmia angustifolia L. Many mechanisms, including allelopathic interference, reduced soil N mineralization, soil enzymes inhibition, and direct resource competition have been proposed to explain poor spruce growth in the presence of Kalmia. However, the relative importance of direct competition versus indirect interference remains unclear. Our objective was thus to adequately isolate the “Kalmia effect” from other growth-limiting factors and to determine if removal of Kalmia also resulted in fundamental changes in the biochemical properties of the forest floor. By sampling plots established in 2000, we evaluated how Kalmia eradication and spot fertilization influenced soil nutrient availability, N mineralization rates, microbial basal respiration and biomass, as well as planted black spruce seedling growth, dimensions, and foliar nutrient concentrations 6 years later. We measured higher extractable-P, mineralizable-N, seedling dimensions and growth rates, as well as lower extractable-K, total-K, basal respiration and microbial biomass, in plots without Kalmia than in those where Kalmia had been maintained from 2000 to 2006. Our results thus confirmed that Kalmia eradication over 6 years not only improved the growth and nutrition of black spruce seedlings, but also resulted in fundamental changes in the biochemical properties of the forest floor. We demonstrated that along with direct competition for resources, Kalmia interferes indirectly with black spruce by modifying nutrient cycling and energy fluxes in soil. Higher indices of available C in plots with Kalmia corroborates that Kalmia tannins or rhizodeposition may reduce N mineralization by stimulating microbial immobilization, a relation that however needs to be confirmed with longer term laboratory incubations. Our results indicated that although it had a positive influence on seedling growth, the fertilization effect was confined to the first few years following treatment application, and failed to influence soil processes as did Kalmia eradication. Further monitoring will indicate if the increased litterfall in fertilized plots will eventually initiate a second wave of fertilizer-induced changes to soil processes, as observed in other ecosystems.     

Variation of wood density and hydraulic properties of Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) clones related to a heat and drought wave in France

Drought response of three Douglas-fir clones (slow, intermediate and fast growing) inhabiting two different climatic regimes in France was examined. We used the hydraulic conductivity and the percent loss of conductivity due to embolism from stems and branches as well as wood microdensity measurements to determine the role of genetics in the control of embolism in this species. Conductivity and tree-ring’s microdensity variables (ring width: RW, mean ring density: MRD, minimum ring density: MID and maximum ring density: MAD) were compared in growth rings in all three clones during a typical year (2002) vs. an extremely dry year (2003). A new method was developed in order to assess axial hydraulic specific conductivity (K_s) within tree rings. The results show that branches are more resistant to embolism than stems, and that there are significant differences in embolism resistance among the clones between the two sites. K_s varied between years, sites and clones but the site exerted the most significant effect. Lartimache (more humid site during 2003) trees showed substantially higher K_s than those inhabiting Chassenoix (strongly affected by the 2003 heat and drought wave site). Wood analysis showed a significant year effect for all ring variables and a significant clone effect for all ring variables except for MAD, while the site effect was significant for MRD and MAD. The existence of a genetic control for the study traits indicates that Douglas-fir populations introduced in France may have a selection potential to face extreme climate events like the 2003 heat and drought wave.     

A commentary on current approaches to forest population genetics

Some of the most frequently chosen approaches to forest population genetics as reflected in the present volume are discussed, and some topics possibly deserving more attention are addressed. Among the first are studies of self-fertilization as a characteristic of forest tree mating systems, gene markers as indicators of adaptive or phylogenetic differentiation, and methodological aspects of the measurement of genetic differentiation. The latter mainly concern generalizable methods of analysis and the involvement of gene markers in the determination of mating systems, detection of mechanisms of frequency dependent selection, and the incorporation of genetic profiles (characteristics of frequency distributions of genetic types) into population genetic analysis.     

Larval traits of the Caribbean amphidromous goby Sicydium punctatum (Gobioidei: Sicydiinae) in Guadeloupe

Amphidromous fish are the biggest contributor to the diversity of fish communities in river systems of Caribbean islands. Among them, Sicydium punctatum Perugia, 1896, which is endemic to the West Indies, represents the vast majority of fish in these rivers. The otolith microstructure and the biometry of S. punctatum postlarvae from Guadeloupe were investigated to explore the dispersal modalities of this species through an appreciation of the growth pattern, the pelagic larval duration (PLD) and the size‐at‐recruitment. The study was made on one cohort of 83 recruited postlarvae, fished at the Capesterre River's mouth on 2 November 2011. The mean (±SD) size‐at‐recruitment of the postlarvae was 24.6 ± 1.3 mm (range of 20.5–28.1 mm, n = 83). We found a mean (±SD) PLD of 72.2 ± 10.5 days (range of 54–101.5 days, n = 67). The growth rate estimated from the otolith increments showed a globally decreasing pattern during the marine larval phase. Growth rates at the beginning of the larval stage were significantly different between hatching periods, suggesting a relation between the hatching period and the growth rate in S. punctatum. This study adds on to the general understanding of the life cycle of S. punctatum in Guadeloupe that will help implement strategies to manage amphidromous fish populations in the Caribbean region.     

Environmental correlates of body size distribution in Cyprinidae (Actinopterygians) depend on phylogenetic scale

The pattern of increasing species body size with increasing latitude has been noticed in different groups of animals. Here, we used seven key environmental factors and independent contrasts to assess body size latitudinal clines in Cyprinidae at two phylogenetic levels (inter‐ and intragenera), which were defined using a genus‐level supertree. Model selection procedures revealed that environmental factors shaping body size variation in Cyprinidae differed according to the phylogenetic scale considered. At the higher phylogenetic level, we found that both temperature (negative effect) and habitat availability (positive effect of drainage basin surface area) constituted mechanistic explanations of large‐scale body size distribution. No temperature‐related body size cline was observed at the intragenus level. Instead, competitive interaction (negative effect of species richness), habitat availability (positive effect of drainage basin surface area), migration ability and available energy (positive effects of glacial coverage and actual evapotranspiration) constitute alternative explanations at this lower phylogenetic scale. We conclude that (i) at the intergenus level, cyprinids do show a tendency to be smaller at high temperatures and larger at low temperatures, (ii) this tendency no longer exists at the intragenus level, (iii) latitude per se is a weak predictor of body size clines whatever the taxonomic level analysed, (iv) generalising geographical body size patterns may be rendered difficult by the superimposition of a series of mechanisms across different taxonomic scales, and (v) habitat size, here acting positively at both taxonomic scales, may play a major role in shaping riverine species body size clines.     

A physical map of the 1-gigabase bread wheat chromosome 3B

As the staple food for 35% of the world's population, wheat is one of the most important crop species. To date, sequence-based tools to accelerate wheat improvement are lacking. As part of the international effort to sequence the 17-billion-base-pair hexaploid bread wheat genome (2n = 6x = 42 chromosomes), we constructed a bacterial artificial chromosome (BAC)-based integrated physical map of the largest chromosome, 3B, that alone is 995 megabases. A chromosome-specific BAC library was used to assemble 82% of the chromosome into 1036 contigs that were anchored with 1443 molecular markers, providing a major resource for genetic and genomic studies. This physical map establishes a template for the remaining wheat chromosomes and demonstrates the feasibility of constructing physical maps in large, complex, polyploid genomes with a chromosome-based approach.