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41.
One hundred and sixty-nine dogs were diagnosed with atopic dermatitis, and treated with hyposensitization for at least 1 year based on the results of either intradermal skin tests (IDST) or enzyme-linked immunosorbant serum assays (ELISA). Excellent (i.e. hyposensitization alone controlled clinical signs), good (> 50% improvement), moderate (< 50% improvement) and no (clinical signs were unchanged) responses were seen in 19.5, 32.5, 20.1 and 27.8%, respectively. Age of onset, age when treatment was initiated or the duration of clinical signs had no influence on response to hyposensitization. Dogs having concurrent flea allergy dermatitis were statistically more likely to respond better than dogs with concurrent food allergies. Although not statistically significant, there were trends for Golden Retriever and male dogs to respond better than other breeds and female dogs, respectively. Dogs having more than 21 positive reactions in allergy tests and treated with more than 21 allergens had lower response scores, and a longer time course before achieving beneficial response. Lower response scores were seen in dogs having positive reactions to cultivated plants, grasses, trees or insects. There was no difference in response to hyposensitization whether based on IDST or ELISA results. 相似文献
42.
Major outer membrane proteins of Brucella spp.: past,present and future 总被引:16,自引:0,他引:16
The major outer membrane proteins (OMPs) of Brucella spp. were initially identified in the early 1980s and characterised as potential immunogenic and protective antigens. They were classified according to their apparent molecular mass as 36–38 kDa OMPs or group 2 porin proteins and 31–34 and 25–27 kDa OMPs which belong to the group 3 proteins. The genes encoding the group 2 porin proteins were identified in the late 1980s and consist of two genes, omp2a and omp2b, which are closely linked in the Brucella genome, and which share a great degree of identity (>85%). In the 1990s, two genes were identified coding for the group 3 proteins and were named omp25 and omp31. The predicted amino acid sequences of omp25 and omp31 share 34% identity. The recent release of the genome sequence of B. melitensis 16 M has revealed the presence of five additional gene products homologous to Omp25 and Omp31. The use of recombinant protein technology and monoclonal antibodies (MAbs) has shown that the major OMPs appear to be of little relevance as antigens in smooth (S) B. abortus or B. melitensis infections i.e. low or no protective activity in the mouse model of infection and low or no immunogenicity during host infection. However, group 3 proteins, in particular Omp31, appear as immunodominant antigen in the course of rough (R) B. ovis infection in rams and as important protective antigen in the B. ovis mouse model of infection. The major OMP genes display diversity and specific markers have been identified for Brucella species, biovars, and strains, including the recent marine mammal Brucella isolates for which new species names have been proposed. Recently, Omp25 has been shown to be involved in virulence of B. melitensis, B. abortus and B. ovis. Mutants lacking Omp25 are indeed attenuated in animal models of infection, and moreover provide levels of protection similar or better than currently used attenuated vaccine strain B. melitensis Rev.1. Therefore, these mutant strains appear interesting vaccine candidates for the future. The other group 3 proteins identified in the genome merit also further investigation related to the development of new vaccines. 相似文献
43.
Anticancer vaccines. 总被引:1,自引:0,他引:1
Philip J Bergman 《Veterinary Clinics of North America: Small Animal Practice》2007,37(6):1111-9; vi-ii
With the tools of molecular biology and a greater understanding of mechanisms to harness the immune system, effective tumor immunotherapy is becoming a reality. This new class of therapeutics offers a more targeted, and therefore precise, approach to the treatment of cancer. The recent conditional licensure of a xenogeneic DNA vaccine for advanced canine malignant melanoma strongly suggests that immunotherapy can play an extremely important role alongside the classic cancer treatment triad components of surgery, radiation therapy, and chemotherapy. 相似文献
44.
Matthew W. Miller DVM Dipl ACVIM Sonya G. Gordon DVM Dipl ACVIM Ashley B. Saunders DVM Dipl ACVIM Wendy G. Arsenault DVM Dipl ACVIM Kathryn M. Meurs DVM Dipl ACVIM Linda B. Lehmkuhl DVM Dipl ACVIM John D. Bonagura DVM Dipl ACVIM Philip R. Fox DVM Dipl ACVIM Dipl ECVIM 《Journal of Veterinary Cardiology》2006,8(2):109-114
45.
Regional land-cover change affects biodiversity, hydrology, and biogeochemical cycles at local, watershed, and landscape scales.
Developing countries are experiencing rapid land cover change, but assessment is often restricted by limited financial resources,
accessibility, and historical data. The assessment of regional land cover patterns is often the first step in developing conservation
and management plans. This study used remotely sensed land cover and topographic data (Landsat and Shuttle Radar Topography
Mission), supervised classification techniques, and spectral mixture analysis to characterize current landscape patterns and
quantify land cover change from 1985 to 2003 in the Altiplano (2535–4671 m) and Intermediate Valley (Mountain) (1491–4623 m)
physiographic zones in the Southeastern Bolivian Andes. Current land cover was mapped into six classes with an overall accuracy
of 88% using traditional classification techniques and limited field data. The land cover change analysis showed that extensive
deforestation, desertification, and agricultural expansion at a regional scale occurred in the last 20 years (17.3% of the
Mountain Zone and 7.2% of the Altiplano). Spectral mixture analysis (SMA) indicated that communal rangeland degradation has
also occurred, with increases in soil and non-photosynthetic vegetation fractions in most cover classes. SMA also identified
local areas with intensive management activities that are changing differently from the overall region (e.g., localized areas
of increased green vegetation). This indicates that actions of local communities, governments, and environmental managers
can moderate the potentially severe future changes implied by the results of this study. 相似文献
46.
47.
William L. Spangler DVM Phd Philip H. Kass 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1997,11(3):166-171
The apparently high prevalence of splenomegaly in dogs, along with the surgical accessibility of the spleen, results in a relatively large number of splenectomies in dogs in clinical veterinary practice. Splenic nodular lesions are widely considered to be indicative of hemangiosarcoma and thus a disease that is ultimately fatal. This study correlates the results of complete pathologic evaluation and classification of 500 spleens obtained by splenectomy with survival information for each dog. Among the spleens examined, 257 of 500 (51.4%) were classified nonneoplastic and 241 (48.2%) were neoplastic; 2 (0.4%) were unclassified. Miscellaneous non-nodular splenomegaly accounted for 46 of 257 (18%) of the nonneoplastic lesions; nodular splenomegaly accounted for 206 of 257 (79%) of nonneoplastic splenic lesions and was composed of lymphoid hyperplastic nodules and associated hematomas, hyperplastic lymphoid nodules alone, or hematomas with no apparent underlying cause. Nodular neoplastic diseases of the spleen were divided among benign tumors (11.5%) and a variety of primary sarcomas. Hemangiosarcoma made up 51% of splenic malignancies but accounted for less than 25% of the spleens evaluated. Survival of dogs with hematomas associated with nonneoplastic conditions of the spleen was markedly different from that in dogs with hemangiosarcoma-associated hematomas, even though most could not be effectively differentiated on gross inspection. Two month postoperative survival was 83% for dogs with nonneoplastic-related hematomas, whereas only 31% of dogs with hemangiosarcoma, with or without associated hematomas, were alive after 2 months. Twelve-month survival times were 64% and 7%, respectively. An overall postsplenectomy survival rate of 52% was based on the number of dogs surviving for a minimum of 6 months postoperatively. 相似文献
48.
Rocky Mountain spotted fever in dogs and its differentiation from canine ehrlichiosis 总被引:4,自引:0,他引:4
C E Greene W Burgdorfer R Cavagnolo R N Philip M G Peacock 《Journal of the American Veterinary Medical Association》1985,186(5):465-472
Rocky Mountain spotted fever (RMSF) or ehrlichiosis was diagnosed in dogs on the basis of specific immunofluorescent testing for each disease. Comparisons between clinical and laboratory findings were made between the 2 diseases. The incidence of RMSF tended to be more seasonal and it affected younger dogs. Purebred dogs appeared to be more susceptible to both diseases. In general, RMSF had a more rapid and severe course of clinical illness than did ehrlichiosis, but acute ehrlichiosis was difficult to differentiate from RMSF. Both diseases were characterized by fever, depression, lymphadenopathy, and signs of neurologic dysfunction; petechial hemorrhages or other signs of hemorrhagic diathesis were evident only in a small proportion of cases. Anemia, leukopenia, and thrombocytopenia were more common in dogs with ehrlichiosis, whereas those with RMSF more often had leukocytosis and thrombocytopenia. Hypoalbuminemia was found in dogs with both diseases, but those with ehrlichiosis usually had concurrent hyperglobulinemia. High serum alkaline phosphatase activity and serum cholesterol concentration, and low serum calcium concentration were more common in dogs with RMSF than with ehrlichiosis. Rising serum titers or positive direct immunofluorescence for Rickettsia rickettsii in skin biopsy specimens were used to confirm RMSF, whereas a single serum titer for Ehrlichia canis enabled detection of ehrlichiosis. In the absence of neurologic deficits and when dogs were treated with tetracycline, dogs with RMSF made a more rapid and consistent recovery than did dogs with ehrlichiosis. 相似文献
49.
50.
Characteristic site disturbance due to harvesting and extraction machinery traffic on sensitive forest sites with peat soils 总被引:2,自引:0,他引:2
Ciaran Nugent Christopher Kanali Philip M. O. Owende Martin Nieuwenhuis Shane Ward 《Forest Ecology and Management》2003,180(1-3):85-98
The effects of wood harvesting and extraction machinery traffic on sensitive forest sites with peat soils were characterised with the objective of quantifying the threshold levels beyond which significant site impacts (compaction and rutting) would occur. The treatments involved running the machines in selected extraction racks (i.e., 3 m wide machine routes) while conducting normal wood thinning and extraction operations comprising one and two passes by the harvester and the forwarder with full payload, respectively. Soil disturbance thresholds were established by testing the level of significance of the difference in induced soil damage and compaction before and after machine traffic treatments. For volumetric soil water content lying between 10.0 and 14.9%, threshold cone penetration resistance levels for two 600/55–30.5 tyres were found to range from 594 to 640 kPa for deep-raised peat soil with initial strength lying between 524 and 581 kPa. In general, the proportion of the total rut depth data in each rack that exceeded the threshold level of 21.5 cm was about 5%. The threshold value corresponds to sinkage equivalent to 15% of the overall wheel diameter of the harvester, above which machine mobility would be hampered considerably. In addition, after harvester traffic the mean rut depth per unit rack length was 10.2×10−2 cm/m, and it ranged from 0.7 to 24.7×10−2 cm/m. 相似文献