Bacterial colonisation in the gut of Phlebotomus duboseqi (Diptera: Psychodidae): transtadial passage and the role of female diet

Bacteria isolated from the gut of different developmental stages of Philebotomus duboseqi Neveu-Lcmaire, 1906 belonged almost all to aerobic or facultatively anaerobic gram-negative rods. In females, the highest bacterial counts were observed two days after bloodfeeding; seven days after bloodfeeding the bacterial counts returned to pre-feeding levels. Most isolates were identified phenotypically as Ochrobactrum sp. The distinctiveness and homogeneity of the phenotypic and genotypic characteristics of Ochrobactrum isolates indicated that they belonged to a single strain (designated AK). This strain was acquired by larvae from food and passaged transtadially: it was isolated from the guts of fourth-instar larvae shortly before pupation, from pupae as well from newly emerged females. Most other bacteria found in females were acquired from the sugar solution fed to adults. To determine if the midgut lectin activity may serve as antibacterial agent females were membrane-fed on blood with addition of inhibitory carbohydrates. No significant differences in bacterial infections were found between experimental and control groups and we suppose that the lectin activity has no effect on gram-negative bacteria present in sandfly gut.     

Cross-sectional imaging assisted selection for heart performance in pigs

A two-way selection procedure was performed on a Landrace pig line. First the cardiac output (CO) values (6.89 +/- 1.27 L/min) were estimated by ECG-gated magnetic resonance imaging (MRI) to quantify the heart performance of the boars (n = 30), then total body skeletal muscle volume (MVTB; 48.7 +/- 5.17 dm3) was determined applying spiral computerised tomography (CT). The relative cardiac output value (RCO) was developed, expressing the relationship between CO and MVTB. Four superior [RCO value below 6.7 dm3 / (L x min(-1))] and one minus variant [(8.97 dm3 / (L x min(-1))] boars were taken into breeding. The best fifteen percentage of the first generation males (n = 30) was characterised according to their RCO values. Significant difference was found between the developed performance measure of the progenies of minus and plus variant boars [8.2 +/- 0.65 and 5.63 +/- 0.70 dm3 / (L x min(-1)), n = 7 and 23], respectively. Comparing the existing five progeny groups, all of the 'HP+' groups differed significantly from the 'HP-' one, where the largest heart performance contrast found was 3.2 dm3 / (L x min(-1)). Based upon the results, the selection is being continued on a larger population.     

Flight patterns of Russian wheat aphid,Diuraphis noxia (Kurdj.) during its expansion to Central Europe (Hom., Aphididae)

Flight patterns of migrant alate Russian wheat aphids (=RWA),Diuraphis noxia (Kurdj.) during its expansion from the south-east to Central Europe were determined. Samples were taken by Rothamsted-type suction air traps in Hungary (one location, 1990–1997) and in the Czech Republic (5 locations, 1994–1998). The results document a progressive expansion of RWA, and population variation in the individual years and periods of the season. A comparison of the evidence on RWA in the individual years since its detection in Hungary and in the Czech Republic supports the presumption on the occurrence of an expansion route from the south-east (Turkey) to Central Europe. This expansion is classified as an adventive route of the over-all expansion that has covered the whole West-Mediterranean (South Europe, North Africa).     

Suction air traps vs. crops in monitoring Russian wheat aphid, Diuraphis noxia (Kurdj.) in Central Europe (Hom., Aphididae)

The Russian wheat aphid (=RWA), Diuraphis noxia (Kurdj.) is an exotic expansive aphid on the small grains determined in the Czech republic since 1993 (1995). Determination of RWA flight patterns by suction air traps (= SATs) between 1994-1998 indicated some banker, less important, or indicated RWA distribution areas. A comparison of the RWA monitoring by SATs and by crop samples in areas more or less distant from individual SAT locations manifested a high silmilarity of RWA evidence in its overall distribution and area-influenced population numbers. Owing to geographic area-dependent features of its life-cycle (holocycly) and host plant preferences, RWA seems to be most injurious to spring barley. In spite of the role of SATs in aphid forecasting, RWA samples are recommended to be taken also from the crops at the early ripening stage as the respective SAT evidence reflects only the emigration of the alate aphids originating from the decreasing populations. In general, RWA detection by SATs was either simultaneously or successively found to reflect the RWA presence in the more or less distant crops. The 30km perimeter of a SAT is agreed to be representative for mon-toring RWA populations in a sampled area.     

Direct detection of the asteroidal YORP effect

The Yarkovsky-O'Keefe-Radzievskii-Paddack (YORP) effect is believed to alter the spin states of small bodies in the solar system. However, evidence for the effect has so far been indirect. We report precise optical photometric observations of a small near-Earth asteroid, (54509) 2000 PH5, acquired over 4 years. We found that the asteroid has been continuously increasing its rotation rate omega over this period by domega/dt = 2.0 (+/-0.2) x 10(-4) degrees per day squared. We simulated the asteroid's close Earth approaches from 2001 to 2005, showing that gravitational torques cannot explain the observed spin rate increase. Dynamical simulations suggest that 2000 PH5 may reach a rotation period of approximately 20 seconds toward the end of its expected lifetime.     

Barley yield response to irrigation and potential profits in the Texas High Plains

Summary Irrigated winter barley (Hordeum vulgare L.) can be a profitable alternative to some low profit major crops in the Texas High Plains. A six-year evaluation of yield response related to total spring irrigation water, applied by surface methods (furrow), and seasonal precipitation resulted in a multivariate function explaining 74% of the yield variation. Predicted yields varied from a low of 3.69 Mg ha^–1 to a maximum 6.18 Mg ha^–1 with 0 and 389 mm, respectively, based on average monthly precipitation quantities. Precipitation is skewed to less than average in th semi-arid Texas High Plains. Using modal precipitation amounts of 40% of average precipitation, yield estimates were reduced to 2.29 Mg ha^–1 with zero spring irrigation and to 5.63 Mg ha^–1 at the peak with 450 mm. A second multivariate yield response function related to alternative timings of single and multiple spring irrigations explained 76% of the variation in yields. Among all combinations of 1, 2, 3, and 4 spring irrigations, irrigation water-use efficiency was estimated to be highest with one application at the boot stage of development. All other single and combinations of multiple irrigations resulted in lower water-use efficiencies. A comparison of enterprise budgets of four irrigation timing alternatives and levels of application indicated highest profit over variable costs, $ 287 ha^–1, was attained by applying a total of 307 mm in three spring applications at the boot, head, and milk stages. A lower level of 217 mm applied at boot and milk stages was $ 12 ha^–1 less profitable and a higher level of 425 mm was $ 24 ha^–1 less profitable. When fixed costs of irrigation facilities, land, and machinery were considered, returns to management and risk were highest, $ 101 ha^–1, with 217 mm. Using 40% of average precipitation, profits were reduced $ 65 ha^–1 with 217 mm and $ 69 ha^–1 with 307 mm spring irrigation levels.Respectively, agricultural economist, research scientist, and research associate, Texas Agr. Exp. Station, Amarillo, Texas; Emeritus Extension agronomist, Texas Agr. Ext. Service, Amarillo, Texas; agricultural engineer, Texas Agr. Exp. Station, Amarillo, Texas     

Two complementary recessive genes conferring resistance to Cucurbit Aphid Borne Yellows Luteovirus in an Indian melon line (cucumis melo L.)

The genetic control of resistance to cucurbit aphid-borne yellows luteovirus (CABYV) in Cucumis melo L. was studied in three progenies obtained from the cross between a resistant Indian line, PI 124112, and a susceptible cultivar of Charentais type, Védrantais. An F2 progeny and recombinant inbred lines were screened respectively in 1993 and 1995 under natural infection conditions in open field at Montfavet, France whereas the back cross (PI 124112 × F1) was inoculated by viruliferous Myzus persicae in controlled conditions. ELISA was used for assessing degree of virus multiplication in the plants resistance. In the three trials performed, segregation analysis were consistent with the hypothesis that the resistance to CABYV in PI 124112 is conferred by two independent complementary recessive genes, for which the symbols cab-1 and cab-2T are proposed. This revised version was published online in July 2006 with corrections to the Cover Date.     

Manganese Biogeochemistry in a Central Czech Republic Catchment

Mn biogeochemistry was studied from 1994 to 2003 in a small forested catchment in the central Czech Republic using the watershed mass balance approach together with measurements of internal stores and fluxes. Mn inputs in bulk deposition were relatively constant during a period of sharply decreasing acidic deposition, suggesting that the Mn source was terrestrial, and not from fossil fuel combustion. Mn inputs in bulk deposition and Mn supplied by weathering each averaged 13 mg m^?2 year^?1 (26 mg m^?2 year^?1 total input), whereas Mn export in streamwater and groundwater averaged 43 mg m^?2 year^?1. Thus an additional Mn source is needed to account for 17 mg m^?2 year^?1. Internal fluxes and pools of Mn were significantly greater than annual inputs and outputs. Throughfall Mn flux was 70 mg m^?2 year^?1, litterfall Mn flux was 103 mg m^?2 year^?1, and Mn net uptake by vegetation was 62 mg m^?2 year^?1. Large pools of labile or potentially labile Mn were present in biomass and surficial soil horizons. Small leakages from these large pools likely supply the additional Mn needed to close the watershed mass balance. This leakage may reflect an adjustment of the ecosystem to recent changes in atmospheric acidity.