Breed associations for canine exocrine pancreatic insufficiency

BACKGROUND: Knowledge of breed associations is valuable to clinicians and researchers investigating diseases with a genetic basis. HYPOTHESIS: Among symptomatic dogs tested for exocrine pancreatic insufficiency (EPI) by canine trypsin-like immunoreactivity (cTLI) assay, EPI is common in certain breeds and rare in others. Some breeds may be overrepresented or underrepresented in the population of dogs with EPI. Pathogenesis of EPI may be different among breeds. ANIMALS: Client-owned dogs with clinical signs, tested for EPI by radioimmunoassay of serum cTLI, were used. METHODS: In this retrospective study, results of 13,069 cTLI assays were reviewed. RESULTS: An association with EPI was found in Chows, Cavalier King Charles Spaniels (CKCS), Rough-Coated Collies (RCC), and German Shepherd Dogs (GSD) (all P < .001). Chows (median, 16 months) were younger at diagnosis than CKCS (median, 72 months, P < .001), but not significantly different from GSD (median, 36 months, P = .10) or RCC (median, 36 months, P = .16). GSD (P < .001) and RCC (P = .015) were younger at diagnosis than CKCS. Boxers (P < .001), Golden Retrievers (P < .001), Labrador Retrievers (P < .001), Rottweilers (P = .022), and Weimaraners (P = .002) were underrepresented in the population with EPI. CONCLUSIONS AND CLINICAL IMPLICATIONS: An association with EPI in Chows has not previously been reported. In breeds with early-onset EPI, immune-mediated mechanisms are possible or the disease may be congenital. When EPI manifests later, as in CKCS, pathogenesis is likely different (eg, secondary to chronic pancreatitis). Underrepresentation of certain breeds among dogs with EPI has not previously been recognized and may imply the existence of breed-specific mechanisms that protect pancreatic tissue from injury.     

Cytokine mRNA profiles in pigs exposed prenatally and postnatally to Schistosoma japonicum

The pig is a natural host for Schistosoma japonicum and a useful animal model of human infection. The aim of the present study was to assess the differences between the cytokine profiles in prenatally or postnatally S. japonicum exposed pigs. Seven prenatally exposed pigs, 7 postnatally exposed pigs and 4 uninfected control pigs were compared 27 weeks post exposure. Variables included worm burdens, tissue egg counts, liver pathology and mRNA levels of IL-2, IL-4, IL-10, IL-12, TNF-alpha, TGF-beta1 and IFN-gamma in the liver and the caecum, assessed by RT-PCR. Infection intensity and level of septal fibrosis were significantly higher in the postnatal group compared to the prenatal group (P < 0.05). A significant increase of IL-4 (P < 0.01), IL-10 (P < 0.01), IL-12 (P < 0.01) and TNF-alpha (P < 0.05) mRNA level was also observed in the caecum of prenatally infected animals compared to the control group (P < 0.01). The prenatal group showed higher levels of TGF-beta1 in the liver compared with the postnatally infected group (P< 0.05) and the control group (P< 0.01). This suppressive immune response correlated with previously reported low hepatic pathogenesis in prenatally exposed pigs.     

Loser cows in Danish dairy herds: definition, prevalence and consequences

During the last few years, many Danish dairy farmers have expressed increasing concerns regarding a group of cows, which we have chosen to term ‘loser cows’. Until now, a loser cow has not been described scientifically. We defined a loser cow on the basis of a clinical examination of the cow. A total of 15,151 clinical examinations were made on 6,451 individual cows from 39 randomly selected, large Danish dairy herds with loose-housing systems using a clinical protocol. Scores for the clinical signs lameness, body condition, hock lesions, other cutaneous lesions, vaginal discharge, condition of hair coat and general condition were converted into a loser cow score. Cows with a loser cow score of 8 or more were classified as loser cows. The overall prevalence of loser cows was 2.15%, 4.50% and 2.98% during the first, second and third round of herd visits, respectively.

The associations between the loser cow state and milk production, mortality, morbidity, culling and workload for the farmer were evaluated using data from herd visits and from the Danish Cattle Database and a number of different statistical techniques. It was concluded that the loser cow state has significant negative consequences for both the farmer and the cow. On average, loser cows yielded 0.61 to 2.24 kg energy corrected milk less per day than non-loser cows depending on parity. Hazard ratio for death or euthanasia was 5.69 for loser cows compared to non-loser cows. Incidence rate ratio for disease treatments was 0.69 for non-loser cows compared to loser cows. Loser cows were often culled in an ‘unfavourable’ way and generally caused extra workload for the farmer.

A simplified version of the loser cow score was evaluated and is recommended for future research and use in practice.     

Acute oral toxicity of zinc phosphide: an assessment for wild house mice (Mus musculus)

Irregular plagues of house mice, Mus musculus, incur major economic impacts on agricultural production in Australia. The efficacy of zinc phosphide (ZnP), the only registered broadacre control agent for mice, is reported as increasingly variable. Have mice become less sensitive over time or are they taking a sub-lethal dose and developing aversion? In this laboratory study, the sensitivity of mice (wild caught; outbred laboratory strain) was assessed using oral gavage of a range of ZnP concentrations. The estimated LD₅₀ values (72–79 mg ZnP/kg body weight) were similar for each mouse group but are significantly higher than previously reported. The willingness of mice to consume ZnP-coated grains was determined. ZnP-coated grains (50 g ZnP/kg grain) presented in the absence of alternative food were consumed and 94% of wild mice died. Mice provided with alternative food and ZnP-coated wheat grains (either 25 or 50 g ZnP/kg grain) consumed toxic and non-toxic grains, and mortality was lower (33–55%). If a sublethal amount of ZnP-coated grain was consumed, aversion occurred, mostly when alternative food was present. The sensitivity of wild house mice to ZnP in Australia is significantly lower than previously assumed. Under laboratory conditions, ZnP-coated grains coated with a new higher dose (50 g ZnP/kg grain) were readily consumed. Consumption of toxic grain occurred when alternative food was available but was decreased. Our unambiguous findings for house mice indicate a re-assessment of the ZnP loading for baits used for control of many rodents around the world may be warranted.     

Inverse Modeling to Quantify Irrigation System Characteristics and Operational Management

Remotely sensed (RS) data is a major source to obtain spatialdata required for hydrological models. The challenge for thefuture is to obtain besides the more direct observable data(landcover, leaf area index, digital elevation model andevapotranspiration), non-visible data such as soilcharacteristics, groundwater depth and irrigation practices.In this study we have explore the option of using inversemodeling to obtain these non-RS-visible data. For a commandarea in Haryana, India, we applied for the 2000–2001 rabiseason a RS-GIS-combined inverse modeling approach to derivenon-RS-visible data required in the regional application ofhydrological models. A Genetic Algorithm loaded stochasticphysically based soil-water-atmosphere-plant model (SWAP) wasdeveloped for the inverse problem and used in the study. Theresults showed good agreement with the inventoried data suchas soil hydraulic properties, sowing dates, groundwaterdepths, irrigation practices and water quality. The deriveddata could be used to predict the state of the system at anytime in the cropping season, which can be used to evaluateoperational management strategies.     

Aquaculture Techniques for Crappie,Pomoxis spp., Culture

Extensive crappie, Pomoxis spp., culture has been practiced for decades, however, knowledge of crappie aquaculture methods is limited. The following review synthesizes existing research on crappie aquaculture and identifies knowledge gaps where further research is needed. Topics such as life history, tank culture, feeding, reproduction and spawning, larval rearing, transport and harvest, triploidy, hybridization, and out‐of‐season spawning were reviewed. The outcome is a better understanding of hindrances preventing crappie aquaculture development in the past, particularly tank culture and induced spawning techniques, and specific research objectives with potential to enhance recreational and commercial production.     

Interspecific differences in early life‐history traits in a species complex of stream‐resident galaxiids

Fish differentially provision resources to eggs and larvae to optimise survival during the critical early life‐history period. Resource allocation is limited by a trade‐off between egg size and fecundity, and the optimal strategy varies with habitat type. This study examines the consequences of egg size differences for the early life‐histories of four closely related galaxiid species which occur in contrasting habitat types on the South Island, New Zealand. Headwater species had substantially larger water‐hardened eggs, longer incubation times, and newly hatched larvae were on average up to 41% longer than lower catchment species. Significant interspecific differences in gape diameter, eye diameter and myomere depth were also observed. Swimming ability was positively associated with larval length at hatch. Interspecific differences in length and swimming ability were generally maintained throughout the larval period, despite larvae being reared under relatively benign conditions where many other studies suggest initial differences should disappear. These results demonstrate the consequences of differential maternal provisioning to the egg for larval traits. The larger larvae of headwater species are likely to be more resistant to starvation and have improved foraging ability compared to lower catchment species; traits which are likely to confer them survival advantages in the low productivity, food‐scarce environments they occupy. The smaller larvae of lower catchment species are likely to be a consequence of females investing in fecundity in these relatively resource‐rich streams.