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21.
OBJECTIVES: To measure serum polymyxin B concentration after single and repeated IV infusions in horses. ANIMALS: 5 healthy horses. PROCEDURES: In study 1, 1 mg (6,000 U) of polymyxin B/kg was given IV and blood samples were collected for 24 hours. In study 2, 1 mg of polymyxin B/kg was given IV every 8 hours for 5 treatments and blood samples were collected until 24 hours after the last dose. Polymyxin B concentration was measured as the ability to suppress nitrite production by murine macrophages stimulated with lipopolysaccharide and interferon-alpha. Urine was collected prior to the first drug infusion and 24 hours after the fifth drug infusion for determination of urinary gamma-glutamyl transferase (GGT)-to-creatinine ratios. RESULTS: In study 1, mean +/- SEM maximal serum polymyxin B concentration was 2.93 +/- 0.38 microg/mL. Polymyxin B was undetectable 18 hours after infusion. In study 2, maximal polymyxin B concentrations after the first and fifth doses were 2.98 +/- 0.81 microg/mL and 1.91 +/- 0.50 microg/mL, respectively. Mean trough concentration for all doses was 0.22 +/- 0.01 microg/mL. A significant effect of repeated administration on peak and trough serum concentration was not detected. Urine GGT-to-creatinine ratios were not affected by polymyxin B administration. CONCLUSIONS AND CLINICAL RELEVANCE: Polymyxin B given as multiple infusions to healthy horses by use of this protocol did not accumulate in the vascular compartment and appeared safe. Results support repeated IV use of 1 mg of polymyxin B/kg at 8-hour intervals as treatment for endotoxemia. 相似文献
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Holdsworth PA Vercruysse J Rehbein S Peter RJ De Bruin C Letonja T Green P;World Association for the Advancement of Veterinary Parasitology 《Veterinary parasitology》2006,136(1):3-13
These guidelines have been prepared to assist in the planning, conduct and interpretation of studies for the assessment of the efficacy of ectoparasiticides (excluding repellents) against the biting and nuisance dipteran flies of ruminants. Information is provided on the selection of animals, dose determination and dose confirmation studies, field studies, record keeping and result interpretation. These guidelines advocate the use of pen facilities for dose determination and dose confirmation studies. These guidelines also are intended to assist investigators on how to conduct specific studies, to provide specific information for registration authorities involved in the decision-making process, to assist in the approval and registration of new ectoparasiticides, and to facilitate the worldwide adoption of standard procedures. 相似文献
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Comparison has been made between Food Green No. 4 and Food Blue No. 3 as tracer dyes for indirect detection of the drugs in milk after intramammary application of a number of commonly used preparations containing antibiotics and chemotherapeutic agents.The tracer dyes had no effect on the stability of the preparations containing penicillin, penicillin ester, and sulphonamide (I, II, III, V and VI) ; after six months’ storage at room temperature, 75 to 100 per cent of the drugs could be recovered. The Oxytetracycline content in preparation IV recovered by 70 to 100 per cent after two months. There were great differences in the irritating effect of the various preparations, as determined by the cell counts of the milk, but the addition of tracer dye did not alter this effect. A direct relationship was demonstrated between concentrations of, on the one hand, tracer dye and, on the other hand, penicillin (preparations I, II, and III), Oxytetracycline (preparation IV) and sulphonamide (preparations V and VI) in milk from ten treated cows.In all quarter samples tracer dye could be demonstrated for just as long as the drugs, and often one or two milkings longer. Thus the experiments confirm the great certainty with which triphenylmethane dyes can reveal indirectly the presence of very small residues of antibiotics and sulphonamides in the milk after intramammary infusion. 相似文献
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A rainfall simulator study of total nutrient movement due to overland flow on forest skid trails and general harvesting areas (GHA) in the native eucalypt forests of southeastern Australia, confirmed that the disturbed and compacted areas were the dominant sources of sediment-bound pollutants. Low runoff production and low sediment availability on the rough surfaces of the GHA reduced total nutrient yields. On the more recently disturbed and burnt sites, the solution pathway may have been more dominant due to runoff coming in contact with surface ash and charcoal. We also recorded significant reductions in the total nutrient yields as runoff from the skid trail was delivered to the adjacent hillslope via a cross bank (water bar). This was due primarily to coarse-grained sediment deposition at the base of the banks. Nutrient concentrations in the eroded sediment at the cross bank outlets, however, remained 9 times higher than the in-situ surface soils, due to the preferential delivery of fine aggregate material to the adjacent hillslope. Further reductions in total nutrient yields occurred as overland flow passed through the GHA, illustrating the effectiveness of vegetative filtering in reducing runoff volumes and sediment fluxes. Temporal trends in the data highlighted significant reductions in total nutrient movement on both areas of disturbance as sites regenerated over a period of ~ 5 years. Water pollution abatement practices in these environments should aim to locate skid trails to maximise the redistribution of runoff and sediment from highly disturbed areas to more vegetated parts of the hillslope, and by appropriate cross bank spacing, reduce the volume of overland flow carrying sediment and nutrients. 相似文献
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Johannes Trini Hans Peter Maurer Sigrid Weissmann Tobias Würschum 《Plant Breeding》2020,139(5):906-915
Accurate hybrid prediction and knowledge about the relative contribution of general (GCA) and specific combining ability (SCA) are of utmost importance for efficient hybrid breeding. We therefore evaluated 91 triticale single-cross hybrids in field trials at seven environments for plant height, heading time, fresh biomass, dry matter content and dry biomass. Fresh and dry biomass showed the highest proportion (23%) of variance due to SCA. Prediction accuracies based on GCA were slightly higher than based on mid-parent values. Utilizing parental kinship information yielded the highest prediction accuracies when both parental lines have been tested in other hybrid combinations, but still moderate-to-low prediction accuracies for two untested parents. Thus, hybrid prediction for biomass traits in triticale is currently promising based on mid-parent values as emphasized by our simulation study, but can be expected to shift to GCA-based prediction with an increasing importance of GCA due to selection in hybrid breeding. Moreover, the performance of potential hybrids between newly developed lines can be predicted with moderate accuracy using genomic relationship information. 相似文献
29.
Functional CpG methylation system in a social insect 总被引:1,自引:0,他引:1
Wang Y Jorda M Jones PL Maleszka R Ling X Robertson HM Mizzen CA Peinado MA Robinson GE 《Science (New York, N.Y.)》2006,314(5799):645-647
DNA methylation systems are well characterized in vertebrates, but methylation in Drosophila melanogaster and other invertebrates remains controversial. Using the recently sequenced honey bee genome, we present a bioinformatic, molecular, and biochemical characterization of a functional DNA methylation system in an insect. We report on catalytically active orthologs of the vertebrate DNA methyltransferases Dnmt1 and Dnmt3a and b, two isoforms that contain a methyl-DNA binding domain, genomic 5-methyl-deoxycytosine, and CpG-methylated genes. The honey bee provides an opportunity to study the roles of methylation in social contexts. 相似文献
30.
Peter R. Brown Rohan Nelson Brent Jacobs Jacquie Tracey Peter DeVoil 《Agricultural Systems》2010,103(8):562-568
We describe the development of a low cost, repeatable self-assessment process enabling community-based natural resource management (CBNRM) groups to set priorities for building their capacity to adopt sustainable farming practices and adapt to global change. Regional measures of adaptive capacity derived from rural livelihoods analysis were populated with secondary data and used to communicate the multiple dimensions of adaptive capacity to groups of landowners. This conceptual framework was then used to derive locally relevant measures of adaptive capacity via focus groups drawn from pre-existing networks of land managers. The key issue discussed at the workshop was what constrained or enabled private land managers to effectively manage natural resources. This self-assessment process was designed to support dialogue between CBNRM groups, industry and governments to prioritise collective action for building adaptive capacity. The approach was piloted with CBNRM groups across New South Wales, Australia. 相似文献