Synthesis of N-substituted dimethylmaleimides and their antifungal activities against Sclerotinia sclerotiorum

Compounds with maleimide, both natural and synthesized, have good biological activities, especially the antifungal activity. In order to investigate the antifungal activity of dimethylmaleimides, 17 N-substituted dimethylmaleimides were prepared from the reactions of 2,3-dimethyl maleic anhydride and amines using a facile synthetic method in this paper. These compounds were evaluated for antifungal activities against Sclerotinia sclerotiorum by the mycelium growth rate method. They exhibited minimum inhibitory concentrations (MICs) ranging from 0.01–50.0 μg/mL, with N-(2-benzimidazole)-3,4-dimethylmaleimide being the most active one with an MIC of 0.01 μg/mL. The structure and activity relationship on these compounds indicated that the hydrophobicity of the N-substituents is associated with their antifungal activity. Compared to current antifungals, most of N-substituted dimethylmaleimides have a perfect activity for S. sclerotiorum control and low toxicity.     

Survey of mycotic mastitis in dairy cows from Heilongjiang Province, China

A survey of the prevalence rate, pathogenic subspecies, and risk factors of mycotic mastitis in dairy cows from Heilongjiang Province, China, was conducted. Milk samples from 412 cows with chronic mastitis were collected and cultured on 8 % sheep blood agar, MacConkey agar, and Sabouraud agar with chloramphenicol. Counting of the morphologically distinct colonies was performed, as well as the isolation and identification of organisms through phenotypical and physiological criteria. Four hundred seventy-eight aerobic microorganisms were isolated. Yeasts and yeast-like fungi 35.6 % (170/478) and bacteria 64.4 % (308/478) were isolated. The fungal isolates were identified as Candida (79.4 %), Trichosporon (5.9 %), Aspergillus (7.1 %), Cryptococcus (2.4 %), and Rhodotorula (4.1 %). More than ten species of yeast were isolated including Candida krusei 50/135 (37 %), Candida rugosa 16/135 (11.9 %), and Candida lusitaniae 15/135 (11.1 %). A higher positivity (18.5 and 56.3 %) (P ≤0.05) was observed in cows from environmental temperatures of 0–15 and 15–35 °C than those at <0 °C and in cows affected by the disease for >45 and 30–45 days compared with cows suffering 10–30 days. Meanwhile, a statistically significant difference (44.9 vs. 31.4 %) (P ≤0.05) was observed under extensive raising systems vs. intensive raising systems. It appears that Candida is a major pathogen of mycotic mastitis of dairy cows. Extensive raising system, high environmental temperature (15–35 °C), and the duration of the disease (>30 days) were important risk factors of the incidence of mycotic mastitis. Here, we provide a theoretical foundation for research into preventing and treating mycotic mastitis of dairy cows in China.     

意蜂蜂王封盖子发育起点温度和有效积温的研究

本文在29℃、31℃、33℃、35℃和37℃,相对湿度40%～60%下,对意大利蜜蜂蜂王封盖子的发育历期、发育起点温度和有效积温进行研究。实验结果显示:①蜂王封盖子的发育期随温度的升高而缩短;②发育起点温度为22.05℃,有效积温为2367.324h℃;③蜂王封盖子的发育期(h)的预测式为N=2367.324/(T-22.05)。     

宁夏动物卫生监督执法工作情况调研报告

为解决基层动物卫生监督执法滞后,基础设施薄弱等问题,全面客观地掌握我区动物卫生监督管理工作现状,笔者对全区动物卫生监督机构情况、畜禽定点屠宰情况、动物及动物产品交易市场情况、动物防疫条件审核发证情况、肉类经营、加工和仓储情况、肉类消费场所检疫监督情况、动物产品（非肉类）加工企业的检疫监督情况、规模饲养场、养殖小区（户）动物卫生监督执法情况等进行了调研,现将调查情况报告如下。     

Assessment and validation of CLIGEN-simulated rainfall data for Northern Taiwan

By using CLIGEN and long-term meteorological data, consecutive daily rainfall data can be simulated. At present, the applicability of CLIGEN to regions other than the United States remains to be tested. In this study, CLIGEN was first applied in a subtropical monsoon climate region. Rainfall data from 1961 to 1990 at three weather stations in northern Taiwan were collected, and rainfall parameters and patterns were validated. The results showed no significant difference in the mean and distribution patterns between observed and simulated values for rainfall parameters including precipitation for each month, wet days for each month, precipitation on wet days for each month and standard deviation of daily precipitation for each month. Regarding rainfall pattern, after re-calibration of storm pattern coefficients, the accuracy of the simulated storm duration was greatly enhanced. However, the simulated daily maximum 5- and 30-min peak intensities were overestimated, and there is room for improvement.     

Effect evaluation of shotcrete vegetation mulching technique applied to steep concrete-face slopes on a highway of Taiwan

The Shotcrete Vegetation Mulching Technique (SVMT) was introduced to revegetate existing steep concrete-face slopes. By mixing cement and greening additives with hydraulic mulching materials in a particular proportion and directly spraying the mixture on steep concrete-face slopes, SVMT was proven to be superior to several traditional methods. SVMT can make a slope surface resistant to erosion within a short time (the uniaxial compressive strength of the slope was higher than 294.3 kN/m² on the 14th day), create an environment suitable for plant growth (the vegetation coverage rate on the 90th day was higher than 90%) and foster a plant community capable of natural succession (competition and succession among Bermudagrass, turf-type tall fescue, hairy beggarticks, chamber bitter, oriental false hawksbeard, American burnweed, Pacific Island silvergrass, and inchplant were observed), effectively improving wildlife habitat and increasing biodiversity (small arthropods were found on the site, including spiders, grasshoppers, ants, ladybirds, etc.). Furthermore, the ecological engineering effects of SVMT on the experiment site were evaluated according to Mitsch and Jørgensen’s 19 Ecological Design Principles. It was found that the site treated with SVMT conformed to principles 2, 3, 8, 10, 11, and 16.     

The reasonable scale of water reuse system in irrigation area: a case study of Chitong irrigation district in Taiwan

The aim of this study is to assess the potential of the irrigation return-flow in a water reuse system, for the supply of other local water users. Both field survey and water-budget analysis were conducted, and the Chitong irrigation district in Taiwan was selected as the case study area. The results indicate that through the regulation of a pond with the effective capacity of 20,000 m³, a stable supply of 10,000 CMD of reuse water can be generated if the return-flow from the irrigation area of 200 hectares, which is about the size of a rotation plot, is intercepted. However, as the irrigation and effective rainfall are low from December to March, which are considered high risk for water supply, the irrigation return-flow decreases accordingly, and a series of responding measures are also suggested.