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991.
珍珠豆型花生品种白沙1016核型分析 总被引:1,自引:0,他引:1
珍珠豆型花生白沙1016是重要的花生种质资源.利用高度保守的重复序列5S和45SrDNA作探针对白沙1016根尖细胞中期染色体进行荧光原位杂交(FISH)分析,发现两探针在白沙1016的12条染色体上能产生杂交信号,其中1对染色体长臂与短臂、2对染色体短臂、3对染色体长臂具有杂交信号.综合DAPI+染色带纹和染色体长度、臂比、面积等特征,可以将白沙1016大部分染色体区分开,为白沙1016的育种利用提供了染色体遗传分析基础. 相似文献
992.
气候变暖对华北冬小麦种植界限及生育期的影响 总被引:5,自引:0,他引:5
为了揭示冬小麦的物候变化态势及其对气候变化的响应程度,以气温的空间变化替代其时间变化,分析了不同升温幅度对华北冬小麦生育期,尤其是对升温较为明显的秋冬季生育期的影响.结果表明,随着气候变暖,冬小麦冬前生育期呈推迟趋势,而越冬之后各生育期呈提早趋势,越冬期缩短.当气温升高1℃时,冬小麦播种时间将推迟4d左右,越冬开始时间由11月底推迟到12月中旬,返青时间由3月初提前到2月中旬,越冬开始至返青的间隔时间将缩短40 d左右;当气温升高1.5℃时,播种时间将推迟10 d左右,越冬时间将由11月底推迟到12月底,个别暖冬年份将推迟至第二年1月初,返青时间提前至2月上中旬,越冬开始至返青的间隔时间将缩短50 d左右;若气温继续上升,越冬开始至返青的间隔时间可能仍会持续地缩短,但缩短的幅度将可能减小. 相似文献
993.
建立复方磺胺间甲氧嘧啶钠可溶性粉中磺胺间甲氧嘧啶钠(以磺胺间甲氧嘧啶计)和甲氧苄啶含量的HPLC检测方法,采用Inertsil C18色谱柱(4.6mm×250mm,5μm),以乙腈:0.017mol/L磷酸液(20:80)(按1‰的比例加入三乙胺)为流动相,流速为1mL/min,检测波长为230nm。在该色谱条件下,两种药物分离很好,磺胺间甲氧嘧啶在24.89~248.9μg/mL之间,甲氧苄啶在5~50μg/mL之间,线性良好(r〉0.99999),添加回收率在97.46%~100.45%之间。该方法快速简便,准确可靠,能够满足实际样品的检测要求。 相似文献
994.
995.
996.
4种牧草苗期耐盐性比较 总被引:1,自引:0,他引:1
用不同NaCl溶液分别对梭罗草(Roegneria thoroldiana)、碱茅(Puccinellia distans)、中华羊茅(Festuca sinensis)和垂穗披碱草(Elymus nutans)进行胁迫处理,研究它们在不同质量分数的NaCl胁迫下植物组织受伤害程度、叶片的叶绿素、可溶性糖、可溶性蛋白质、游离脯氨酸的含量及过氧化物酶、过氧化氢酶活性的变化。结果表明,在盐质量分数为0.2%时,4种供试牧草的抗盐性依次为碱茅>中华羊茅>梭罗草>垂穗披碱草;在质量分数为0.4%~1.0%时,抗盐性依次为碱茅>梭罗草>中华羊茅>垂穗披碱草。 相似文献
997.
998.
? Context
A clear understanding of the genetic control of wood properties is a prerequisite for breeding for higher wood quality in Populus tomentosa Carr. hybrid clones.? Aims
The experiments aimed at unraveling genetic and environmental effects on wood properties among triploid hybrid clones of P. tomentosa.? Methods
We used 5-year-old clonal trials established in Northern China to assess the heritability of wood density and fiber traits. Two hundred seventy trees from nine clones were sampled in five sites.? Results
Site had a very significant effect on all recorded traits. Despite this large site effect, a tight genetic control was detected and clonal repeatability varied between 0.53 and 0.95. Significant genotype?×?environment interactions were detected for most of the traits. Moderate to tight correlation were evidenced among traits but they were not consistent with that in several cases that were site-dependent.? Conclusions
Our results revealed a tight genetic control over several wood properties and therefore breeding programs might be able to improve wood density, fiber length, and coarseness in these hybrids. 相似文献999.
Yulong Zhou Yachao Ren Chunling Fan Hong Shao Zecai Zhang Wenbin Mao Chunbo Wei Hongbo Ni Zhanbo Zhu Xilin Hou Fanze Piao Yudong Cui 《Tropical animal health and production》2013,45(8):1709-1714
A survey of the prevalence rate, pathogenic subspecies, and risk factors of mycotic mastitis in dairy cows from Heilongjiang Province, China, was conducted. Milk samples from 412 cows with chronic mastitis were collected and cultured on 8 % sheep blood agar, MacConkey agar, and Sabouraud agar with chloramphenicol. Counting of the morphologically distinct colonies was performed, as well as the isolation and identification of organisms through phenotypical and physiological criteria. Four hundred seventy-eight aerobic microorganisms were isolated. Yeasts and yeast-like fungi 35.6 % (170/478) and bacteria 64.4 % (308/478) were isolated. The fungal isolates were identified as Candida (79.4 %), Trichosporon (5.9 %), Aspergillus (7.1 %), Cryptococcus (2.4 %), and Rhodotorula (4.1 %). More than ten species of yeast were isolated including Candida krusei 50/135 (37 %), Candida rugosa 16/135 (11.9 %), and Candida lusitaniae 15/135 (11.1 %). A higher positivity (18.5 and 56.3 %) (P ≤0.05) was observed in cows from environmental temperatures of 0–15 and 15–35 °C than those at <0 °C and in cows affected by the disease for >45 and 30–45 days compared with cows suffering 10–30 days. Meanwhile, a statistically significant difference (44.9 vs. 31.4 %) (P ≤0.05) was observed under extensive raising systems vs. intensive raising systems. It appears that Candida is a major pathogen of mycotic mastitis of dairy cows. Extensive raising system, high environmental temperature (15–35 °C), and the duration of the disease (>30 days) were important risk factors of the incidence of mycotic mastitis. Here, we provide a theoretical foundation for research into preventing and treating mycotic mastitis of dairy cows in China. 相似文献
1000.
Fei Zhou Liancheng Cui Zhiqiang Huang Shuang Tong Meng Yu Rongchang Liu Zhangyong Ning 《Veterinary research communications》2013,37(4):285-291
In chicken, ST3 beta-galactoside alpha-2, 3-sialyltransferase III (ST3GAL III) is one of the key enzymes participating in the biosynthesis of avian influenza virus receptors. Knowledge about ST3Gal III could increase our understanding of its function in the occurrence and development of avian influenza. To date, no detailed data have been published about the expression pattern and histological distribution of ST3Gal III in chicken. This paper presents data on the nucleotide sequence, mRNA expression pattern and histological distribution of ST3Gal III protein in yellow chicken for the first time. The results show that the nucleotide homology of ST3Gal III among yellow chicken and turkey, chicken, cattle, humans, swine, mouse, rat and chimpanzee was 98 %, 92 %, 75 %, 59 %, 70 %, 76 % and 75 %. The relative expression level of ST3Gal III in the heart, kidney and brain of yellow chicken was significantly higher than in other tissues examined for mRNA level (P?<?0.05). The histological distribution of ST3Gal III in the heart, liver, spleen, lung, thymus and bursa of Fabricius was determined by immunohistochemical staining using rabbit anti-chicken ST3Gal III IgG prepared by us. Interestingly, the epithelial reticular cells in the immune organs proved to be positive, which may suggest that these cells are important immune cells playing a role in influenza virus infection. The results of this study provide basic data for further research on the functions of ST3Gal III in chicken. 相似文献