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61.
Katharina Bratz Roland Bücker Greta Gölz Silke S. Zakrzewski Pawel Janczyk Karsten Nöckler Thomas Alter 《Veterinary microbiology》2013
Campylobacter (C.) is one of the most common food-borne pathogen causing bacterial enteric infections in humans. Consumption of meat and meat products that have been contaminated with Campylobacter are the major source of infection. Pigs are a natural reservoir of Campylobacter spp. with C. coli as the dominant species. Even though some studies focussed on transmission of C. coli in pig herds and the excretion in faeces, little is known about the colonisation and excretion dynamics of C. coli in a complex gut microbiota present in weaned piglets and the translocation to different tissues. 相似文献
62.
Fijalkowski K Czernomysy-Furowicz D Irwin JA Nawrotek P Pobucewicz A 《Research in veterinary science》2012,93(1):82-87
The aim of the research was to test whether exogenic virulence factors secreted by Staphylococcus aureus isolates are involved in mechanisms that allow the bacteria to modulate and evade phagocytosis by bovine polymorphonuclear neutrophils. The research was based on the comparison of the effects of supernatants, prepared from cultures of 30 S. aureus isolates, on the functional properties of bovine neutrophils in vitro. S. aureus isolates were collected from milk samples from cows with clinical mastitis. Supernatants, which were used to treat leukocytes, were prepared from 18 h S. aureus cultures. Exogenic virulence factors secreted by S. aureus isolates significantly influenced the phagocytosis parameters evaluated. Depending on their leukotoxic or superantigenic properties, supernatants could affect the ingestion process, and also showed an influence on the digestion efficiency and phagocytosis carried out by bovine polymorphonuclear neutrophils in vitro. 相似文献
63.
Phosphonic analogues of morphactins. Part V: Peptides containing 9-aminofluoren-9-ylphosphine oxides
Pawel Kafarski Barbara Lejczak Roman Gancarz Elbieta Jaskulska Przemyslaw Mastalerz Jan Sylwester Wieczorek Iwona Zbyryt 《Pest management science》1985,16(3):239-243
Di- and tripeptides containing P-terminal 9-aminofluoren-9-ylphosphine oxides were synthesised and evaluated for herbicidal activity using Spirodela oligorriza (Kurz.) Hegelm. The glycylglycyl and threonyl peptides showed high activity as measured by the reduction of dry matter. 相似文献
64.
Piotr Wieczorek Barbara Lejczak Malgorzata Kaczanowska Pawel Kafarski 《Pest management science》1990,30(1):43-57
Phosphono peptides exhibited plant-growth-regulating activity when tested on Lepidium sativum and Cucumis sativus. The studies have provided evidence that the mechanism of action of these compounds may involve the uptake of the phosphono peptides into the plant, intracellular hydrolysis of the peptide bond and release of the P-terminal aminoalkylphosphonic acid. This acid or its metabolite is then probably responsible for the observed physiological effect. 相似文献
65.
Acute BVDV-2 infection in beef calves delays humoral responses to a non-infectious antigen challenge
UCVM Class of Robert McCorkell Shawn R. Horsman Katherine Wynne-Edwards Greg Muench Sylvia van Drunen Littel-van den Hurk Regula Waeckerlin Michael Eschbaumer Rkia Dardari Mark Chaiyakul Pawel Gajda Markus Czub Frank van der Meer 《The Canadian veterinary journal. La revue veterinaire canadienne》2015,56(10):1075-1083
Immunosuppressive effects of an intranasal challenge with non-cytopathic bovine viral diarrhea virus (BVDV) 2a (strain 1373) were assessed through acquired and innate immune system responses to ovalbumin (OVA). Concurrent BVDV infection was hypothesized to delay and reduce the humoral response to ovalbumin (administered on days 3 and 15 post-inoculation). Infected animals followed the expected clinical course. BVDV titers, and anti-BVDV antibodies confirmed the course of infection and were not affected by the administration of OVA. Both the T-helper (CD4+) and B-cell (CD20+) compartments were significantly (P < 0.05) reduced in infected animals, while the gamma-delta T-cell population (Workshop cluster 1+, WC1+) decreased slightly in numbers. Infection with BVDV delayed the increase in OVA IgG by approximately 3 d from day 12 through day 21 post-inoculation. Between days 25 and 37 post-inoculation following BVDV infection the IgM concentration in the BVDV− group decreased while the OVA IgM titer still was rising in the BVDV+ animals. Thus, active BVDV infection delays IgM and IgG responses to a novel, non-infectious antigen. 相似文献
66.
Effect of feeding various levels of poultry by‐product meal on the blood parameters,filet composition and structure of female tenches (Tinca tinca)
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Remigiusz Panicz Joanna Żochowska‐Kujawska Jacek Sadowski Małgorzata Sobczak 《Aquaculture Research》2017,48(10):5373-5384
Homogeneous background (age, sex, genetic lineage, culture conditions) was created to clearly demonstrate the impact of the tested dietary treatments. No feeds optimized for the rearing of the tench (Tinca tinca L. 1758) are available. Feeds are formulated to increase the growth rate or eliminate skeletal deformations. With the increasing prices of the basic components, fish meal (FM) and fish oil, poultry by‐product meal (PBM) can be used. The aim of the study was to evaluate the effects of substitution of FM with PBM on the tench blood parameters, body composition and structure of skeletal muscles. Cage‐reared female tenches (325 ± 18 g) were fed for 86 days with five types of feeds with 0% (control), 25.7%, 48.6%, 71.4% and 100% substitution of FM with PBM. No significant differences between the formulations were reported for weight, total length, fillet weight, visceral, liver, gonado‐somatic, proximate composition and biochemical blood parameters. However, significant differences were found in the fillet profiles of fatty acids—an increase in the PBM content correlated with an increase in saturated and monounsaturated fatty acids and a decrease in n‐3 PUFA and, generally, n‐6 PUFA. Significant differences were also observed in, for example, the content of intramuscular fatty tissue and the level of organ lipidosis between the control variant (0% PBM) and that with 100% substitution. A sensory assessment indicated a higher gustatory value of the fillets in the case of feeds with 48.6% and 71.4% substitution. 相似文献
67.
S M Bergmann J Sadowski M Kiełpiński M Bartłomiejczyk D Fichtner R Riebe M Lenk J Kempter 《Journal of fish diseases》2010,33(3):267-272
Hybrids of koi, Cyprinus carpio × crucian carp, Carassius carassius and koi × goldfish, Carassius auratus, proved to be susceptible to koi herpesvirus (KHV, syn. CyHV‐3) and developed KHV disease (KHVD). While hybrids of koi × goldfish were partly resistant to mortality following infection by immersion, most koi × crucian carp hybrids died after bath infection. KHV DNA was detected in dead fish but also in all surviving animals by different polymerase chain reactions (PCRs). According to these results, hybrid crossbreeding does not seem to prevent severe losses associated with KHV in terms of inducing KHVD. The present study showed severe losses after a waterborne KHV infection of between 35% and 100% in koi × goldfish and koi × crucian carp hybrids as well as in SPF carp. 相似文献
68.
Ten microsatellite loci were tested on Mendelian segregation in the bester – hybrid of beluga, Huso huso L. and sterlet, Acipenser ruthenus L. in the fourth generation. All studied loci showed disomic inheritance and Mendelian segregation of their alleles. The molecular approach by microsatellite DNA analysis represents a reliable tool for interspecific characterization of sturgeons and their hybrids (different alleles of several loci are present in sturgeon fishes, and interspecific hybrids posesses both maternal and paternal specific alleles). This is the first report of the Mendelian segregation testing in interspecific hybrid of acipenserid fishes using microsatellite DNA markers. 相似文献
69.
Piotr Kupczyk Marta Rykala Pawel Serek Aleksandra Pawlak Bartosz Slowikowski Marcin Holysz Grzegorz Chodaczek Jan P. Madej Piotr Ziolkowski Artur Niedzwiedz 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2022,36(4):1508
BackgroundThe endocannabinoid system (ECS) is composed of cannabinoid receptors type 1 (CBR1) and type 2 (CBR2), cannabinoid‐based ligands (endogenous chemically synthesized phytocannabinoids), and endogenous enzymes controlling their concentrations. Cannabinoid receptors (CBRs) have been identified in invertebrates and in almost all vertebrate species in the central and peripheral nervous system as well as in immune cells, where they control neuroimmune homeostasis. In humans, rodents, dogs, and cats, CBRs expression has been confirmed in the skin, and their expression and tissue distribution become disordered in pathological conditions. Cannabinoid receptors may be a possible therapeutic target in skin diseases.ObjectivesTo characterize the distribution and cellular expression of CBRs in the skin of horses under normal conditions.AnimalsFifteen healthy horses.MethodsUsing full‐thickness skin punch biopsy samples, skin‐derived primary epidermal keratinocytes and dermal‐derived cells, we performed analysis of Cnr1 and Cnr2 genes using real‐time PCR and CBR1 and CBR2 protein expression by confocal microscopy and Western blotting.ResultsNormal equine skin, including equine epidermal keratinocytes and dermal fibroblast‐like cells, all exhibited constant gene and protein expression of CBRs.Conclusions and Clinical ImportanceOur results represent a starting point for developing and translating new veterinary medicine‐based pharmacotherapies using ECS as a possible target. 相似文献
70.
Chromosome preparations and assay of the microsatellite locus Afu‐68 were used to determine ploidy in Siberian sturgeon (Acipenser baeri Brandt) and F1 hybrids of Siberian sturgeon and Russian sturgeon (Acipenser gueldenstaedti Brandt). The chromosome number and microsatellite locus Afu‐68 were compared and these analyses were used for identification of ‘haploid’, ‘diploid’ and ‘triploid’ progeny of the studied cross of A. baeri× (A. baeri×A. gueldenstaedti). 相似文献