全文获取类型
收费全文 | 4182篇 |
免费 | 205篇 |
国内免费 | 13篇 |
专业分类
林业 | 267篇 |
农学 | 149篇 |
基础科学 | 33篇 |
807篇 | |
综合类 | 661篇 |
农作物 | 244篇 |
水产渔业 | 388篇 |
畜牧兽医 | 1404篇 |
园艺 | 136篇 |
植物保护 | 311篇 |
出版年
2023年 | 35篇 |
2022年 | 26篇 |
2021年 | 90篇 |
2020年 | 93篇 |
2019年 | 101篇 |
2018年 | 88篇 |
2017年 | 87篇 |
2016年 | 93篇 |
2015年 | 68篇 |
2014年 | 102篇 |
2013年 | 186篇 |
2012年 | 241篇 |
2011年 | 284篇 |
2010年 | 185篇 |
2009年 | 146篇 |
2008年 | 234篇 |
2007年 | 256篇 |
2006年 | 228篇 |
2005年 | 236篇 |
2004年 | 227篇 |
2003年 | 216篇 |
2002年 | 199篇 |
2001年 | 58篇 |
2000年 | 60篇 |
1999年 | 54篇 |
1998年 | 38篇 |
1997年 | 30篇 |
1996年 | 40篇 |
1995年 | 31篇 |
1994年 | 33篇 |
1993年 | 45篇 |
1992年 | 33篇 |
1991年 | 39篇 |
1990年 | 32篇 |
1989年 | 45篇 |
1988年 | 31篇 |
1987年 | 22篇 |
1986年 | 30篇 |
1985年 | 27篇 |
1984年 | 34篇 |
1983年 | 21篇 |
1982年 | 13篇 |
1981年 | 16篇 |
1979年 | 16篇 |
1978年 | 13篇 |
1977年 | 11篇 |
1976年 | 12篇 |
1975年 | 17篇 |
1974年 | 17篇 |
1973年 | 16篇 |
排序方式: 共有4400条查询结果,搜索用时 15 毫秒
21.
Antibodies to Coxiella burnetii have been found in the Danish dairy cattle population with high levels of herd and within herd seroprevalences. However, the prevalence of antibodies to C. burnetii in Danish beef cattle remains unknown. The objectives of this study were to (1) estimate the prevalence and (2) identify risk factors associated with C. burnetii seropositivity in Danish beef and dairy cattle based on sampling at slaughter. 相似文献
22.
Steed PM Tansey MG Zalevsky J Zhukovsky EA Desjarlais JR Szymkowski DE Abbott C Carmichael D Chan C Cherry L Cheung P Chirino AJ Chung HH Doberstein SK Eivazi A Filikov AV Gao SX Hubert RS Hwang M Hyun L Kashi S Kim A Kim E Kung J Martinez SP Muchhal US Nguyen DH O'Brien C O'Keefe D Singer K Vafa O Vielmetter J Yoder SC Dahiyat BI 《Science (New York, N.Y.)》2003,301(5641):1895-1898
Tumor necrosis factor (TNF) is a key regulator of inflammatory responses and has been implicated in many pathological conditions. We used structure-based design to engineer variant TNF proteins that rapidly form heterotrimers with native TNF to give complexes that neither bind to nor stimulate signaling through TNF receptors. Thus, TNF is inactivated by sequestration. Dominant-negative TNFs represent a possible approach to anti-inflammatory biotherapeutics, and experiments in animal models show that the strategy can attenuate TNF-mediated pathology. Similar rational design could be used to engineer inhibitors of additional TNF superfamily cytokines as well as other multimeric ligands. 相似文献
23.
24.
25.
Chen Y Corriden R Inoue Y Yip L Hashiguchi N Zinkernagel A Nizet V Insel PA Junger WG 《Science (New York, N.Y.)》2006,314(5806):1792-1795
Cells must amplify external signals to orient and migrate in chemotactic gradient fields. We find that human neutrophils release adenosine triphosphate (ATP) from the leading edge of the cell surface to amplify chemotactic signals and direct cell orientation by feedback through P2Y2 nucleotide receptors. Neutrophils rapidly hydrolyze released ATP to adenosine that then acts via A3-type adenosine receptors, which are recruited to the leading edge, to promote cell migration. Thus, ATP release and autocrine feedback through P2Y2 and A3 receptors provide signal amplification, controlling gradient sensing and migration of neutrophils. 相似文献
26.
Peter M. Kiffney Ben Cram Paul L. Faulds Karl Burton Michele Koehler Thomas P. Quinn 《Ecology of Freshwater Fish》2018,27(4):1037-1053
To examine the role of longitudinal connectivity on the spatial and temporal dynamics of mountain whitefish (Prosopium williamsoni), we quantified movement and population dynamics following installation of the Landsburg Dam fishway, Cedar River, WA, USA. Mountain whitefish is widely distributed, poorly studied and not the focus of restoration. Before the fishway, mountain whitefish were not observed above the dam. Here, we focus on snorkel counts collected at reach and mesohabitat (e.g. pools) scales over 11 summers on the 20‐km above‐dam segment following restoration. A camera within the ladder provided number, size and movement timing, thereby informing on behaviour and recolonisation. Segment‐scale abundance increased following fish passage reaching an asymptote in 7 years, and mountain whitefish were detected throughout the main stem in 10 years. Annual movement through the ladder increased over time and was positively correlated with instream abundance and discharge, but negatively correlated with water temperature. About 60% of fish movements occurred in spring and early summer, potentially for foraging opportunities. Reach‐scale abundance peaked between 7 and 10 km from the dam; deep, cool (~10.6 to 11.6°C) conditions characterised these reaches. At the mesohabitat scale, mountain whitefish detection increased with depth and velocity after accounting for distance from the dam. Our results show how restoring longitudinal connectivity allowed this nontarget species to colonise newly available habitat. Their response supports the critical roles of longitudinal connectivity and environmental conditions, that manifest at different spatial scales, in dictating how freshwater fish respond to habitat disturbance. 相似文献
27.
Rebecca C. Bott Elizabeth A. Greene Katherine Koch Krishona L. Martinson Paul D. Siciliano Carey Williams Nathalie L. Trottier Amy Burk Ann Swinker 《Journal of Equine Veterinary Science》2013
Horses' physical and digestive well-being is often enhanced when allowed to graze on pastures. Furthermore, a well-managed pasture can contribute to economic viability. Grazing can however have deleterious effects on the environment if not properly managed. Although equine grazing, defecating, and ground trampling behavior is unique from that of other livestock species, pasture management practices are often based on those derived from cattle grazing. This review summarizes the current knowledge of impacts of equine grazing on pasture quality and environment and identifies gaps where further information is needed to formulate and recommend sustainable grazing methods specific to equine. 相似文献
28.
29.
从扁藻超微结构变化研究CuZnSeCd离子毒性效应 总被引:7,自引:1,他引:7
使用透射电镜研究了在半抑制效应的Cu、Zn、Se、Cd无机离子的毒性作用下,扁藻细胞超微结构的变化。证明不同离子及不同离子浓度对扁藻的毒性效应不同。从细胞器的变化来看,扁藻的叶绿体和细胞核对上述4种离子都较为敏感。其中Cd离子对叶绿体和细胞核的毒性效应表现最为明显:而Cu离子对上述两种细胞器的毒性作用相对较弱;Se离子对叶绿体,Zn离子对细胞核的破坏作用相对较小,Se离子对细胞核,Zn离子对叶绿体 相似文献
30.
Paul Chittaro Jens Hegg Abby Fuhrman Devin Robichaux Rothboury Doung Brian Beckman Chris Fisher Jeff Vervoort Brian Kennedy 《Ecology of Freshwater Fish》2023,32(1):107-119
Effective species management often requires understanding patterns of movement and habitat use. A common approach in identifying where individuals reside relies upon chemical tracers from the environment that are incorporated into an individual's tissues. For fish, isotopes in their otoliths, specifically the portion of their otolith formed during their larval stage, have been used to identify the natal origin. Complicating this work, however, is the fact that during this life stage, there is a shift in the source of isotopes deposited onto the growing otolith from maternally to environmentally derived. The objective of this study was to identify the portion of the otolith representing this transition to environmentally derived isotopes so as to accurately investigate questions of natal origin for a threatened population of fall Chinook salmon (Oncorhynchus tshawytscha). We exposed developing larvae to four treatments that differed in terms of their water strontium isotope ratio (87Sr/86Sr) and used change-point analysis of otolith 87Sr/86Sr and strontium to calcium ratio (Sr/Ca) to identify the otolith radius corresponding to the transition to environmentally derived isotopes. Our results indicated this transition occurred, on average, at 132 μm (87Sr/86Sr; ±50 μm standard deviation) and 127 μm (Sr/Ca; ±29 μm) from the otolith core, which corresponded to the developmental time between hatching and exogenous feeding. A substantial proportion of our otoliths (i.e., 61%) did not show convergence between otolith and water 87Sr/86Sr by the end of the 113-day experiment, which was likely due to the dietary contribution of marine-based feed. Therefore, we were unable to recommend an otolith radius to target for the purposes of reconstructing natal origin apart from being beyond approximately 130 μm. 相似文献