全文获取类型
收费全文 | 4241篇 |
免费 | 217篇 |
国内免费 | 13篇 |
专业分类
林业 | 270篇 |
农学 | 152篇 |
基础科学 | 33篇 |
806篇 | |
综合类 | 671篇 |
农作物 | 242篇 |
水产渔业 | 390篇 |
畜牧兽医 | 1455篇 |
园艺 | 139篇 |
植物保护 | 313篇 |
出版年
2023年 | 35篇 |
2022年 | 24篇 |
2021年 | 90篇 |
2020年 | 94篇 |
2019年 | 102篇 |
2018年 | 88篇 |
2017年 | 88篇 |
2016年 | 95篇 |
2015年 | 66篇 |
2014年 | 108篇 |
2013年 | 189篇 |
2012年 | 252篇 |
2011年 | 284篇 |
2010年 | 184篇 |
2009年 | 148篇 |
2008年 | 238篇 |
2007年 | 255篇 |
2006年 | 229篇 |
2005年 | 237篇 |
2004年 | 230篇 |
2003年 | 216篇 |
2002年 | 204篇 |
2001年 | 59篇 |
2000年 | 59篇 |
1999年 | 55篇 |
1998年 | 36篇 |
1997年 | 30篇 |
1996年 | 42篇 |
1995年 | 32篇 |
1994年 | 33篇 |
1993年 | 45篇 |
1992年 | 37篇 |
1991年 | 43篇 |
1990年 | 36篇 |
1989年 | 41篇 |
1988年 | 34篇 |
1987年 | 26篇 |
1986年 | 33篇 |
1985年 | 30篇 |
1984年 | 35篇 |
1983年 | 23篇 |
1982年 | 13篇 |
1981年 | 16篇 |
1979年 | 17篇 |
1978年 | 13篇 |
1977年 | 13篇 |
1976年 | 13篇 |
1975年 | 17篇 |
1974年 | 17篇 |
1973年 | 18篇 |
排序方式: 共有4471条查询结果,搜索用时 15 毫秒
71.
Paul Dijkstra Ayaka Ishizu Stephen C. Hart Egbert Schwartz Bruce A. Hungate 《Soil biology & biochemistry》2006,38(11):3257-3266
Stable isotope analysis is a powerful tool in the study of soil organic matter formation. It is often observed that more decomposed soil organic matter is 13C, and especially 15N-enriched relative to fresh litter and recent organic matter. We investigated whether this shift in isotope composition relates to the isotope composition of the microbial biomass, an important source for soil organic matter. We developed a new approach to determine the natural abundance C and N isotope composition of the microbial biomass across a broad range of soil types, vegetation, and climates. We found consistently that the soil microbial biomass was 15N-enriched relative to the total (3.2 ‰) and extractable N pools (3.7 ‰), and 13C-enriched relative to the extractable C pool (2.5 ‰). The microbial biomass was also 13C-enriched relative to total C for soils that exhibited a C3-plant signature (1.6 ‰), but 13C-depleted for soils with a C4 signature (−1.1 ‰). The latter was probably associated with an increase of annual C3 forbs in C4 grasslands after an extreme drought. These findings are in agreement with the proposed contribution of microbial products to the stabilized soil organic matter and may help explain the shift in isotope composition during soil organic matter formation. 相似文献
72.
以白三叶(Trifolium repens L.)的两个品种Irrigation和Huia的子叶为转化体,用根癌农杆菌(Agrobacterium tumefaciens)介导法将外源的苜蓿花叶病毒外壳蛋白基因转入白三叶,经过筛选、分化和再生,得到了具有卡拉霉素抗性的再生植株。对这些植株进行PCR、Southern和Northern分析,结果表明,外源目的基因已经整合到白三叶基因组中并且得到了表达。对Northern分析呈阳性的植株进行了抗病性检测,证明表达苜蓿花叶病毒外壳蛋白基因的植株病症减轻,发病率、病情指数及病毒积累量都明显低于对照,有的甚至不表现症状,达到了免疫的程度。 相似文献
73.
Wynand J. Goosen Lanie Kleynhans Tanya J. Kerr Paul D. van Helden Peter Buss Robin M. Warren Michele A. Miller 《Journal of veterinary diagnostic investigation》2022,34(1):61
In South Africa, mycobacterial culture is regarded as the gold standard for the detection of Mycobacterium tuberculosis complex (MTBC) infection in wildlife even though it is regarded as “imperfect.” We compared a novel decontamination and mycobacterial culture technique (TiKa) to the conventional mycobacterium growth indicator tube (MGIT) system using known amounts of bacilli and clinical samples from MTBC-infected African buffaloes (Syncerus caffer), white rhinoceros (Ceratotherium simum), and African elephants (Loxodonta africana). Use of the TiKa-KiC decontamination agent on samples spiked with 10,000 to 10 colony forming units (cfu) of M. bovis (SB0121) and M. tuberculosis (H37Rv) had no effect on isolate recovery in culture. In contrast, decontamination with MGIT MycoPrep resulted in no growth of M. bovis samples at concentrations < 1,000 cfu and M. tuberculosis samples < 100 cfu. Subsequently, we used the TiKa system with stored clinical samples (various lymphatic tissues) collected from wildlife and paucibacillary bronchoalveolar lavage fluid, trunk washes, and endotracheal tube washes from 3 species with known MTBC infections. Overall, MTBC recovery by culture was improved significantly (p < 0.01) by using TiKa compared to conventional MGIT, with 54 of 57 positive specimens versus 25 of 57 positive specimens, respectively. The TiKa mycobacterial growth system appears to significantly enhance the recovery of MTBC members from tissue and paucibacillary respiratory samples collected from African buffaloes, African elephants, and white rhinoceros. Moreover, the TiKa system may improve success of MTBC culture from various sample types previously deemed unculturable from other species. 相似文献
74.
75.
76.
77.
Zhai Changyuan Long John Taylor Randal Weckler Paul Wang Ning 《Precision Agriculture》2020,21(3):589-602
Precision Agriculture - With recent developments allowing increased planter speeds, improving spatial uniformity of crop emergence will continue to be an important focus of research. Vertical... 相似文献
78.
Vimal Nair Min Cheol Kim James A. Golen Arnold L. Rheingold Gabriel A. Castro Paul R. Jensen William Fenical 《Marine drugs》2020,18(11)
A new cytotoxic thiodepsipeptide, verrucosamide (1), was isolated along with the known, related cyclic peptide thiocoraline, from the extract of a marine-derived actinomycete, a Verrucosispora sp., our strain CNX-026. The new peptide, which is composed of two rare seven-membered 1,4-thiazepane rings, was elucidated by a combination of spectral methods and the absolute configuration was determined by a single X-ray diffraction study. Verrucosamide (1) showed moderate cytotoxicity and selectivity in the NCI 60 cell line bioassay. The most susceptible cell lines were MDA-MB-468 breast carcinoma with an LD50 of 1.26 µM, and COLO 205 colon adenocarcinoma with an LD50 of 1.4 µM. Also isolated along with verrucosamide were three small 3-hydroxy(alkoxy)-quinaldic acid derivatives that appear to be products of the same biosynthetic pathway. 相似文献
79.
Foltête Jean-Christophe Savary Paul Clauzel Céline Bourgeois Marc Girardet Xavier Sahraoui Yohan Vuidel Gilles Garnier Stéphane 《Landscape Ecology》2020,35(5):1053-1053
Landscape Ecology - In the original publication of the article, the sixth author name has been misspelt. The correct name is given in this Correction. The original article has been corrected. 相似文献
80.
Brian N. Hogg Keith Stokes Min B. Rayamajhi John Geiger Paul D. Pratt 《Weed Research》2020,60(3):212-220
Schinus terebinthifolia is a dioecious tree native to South America that has become an invasive weed in Florida, southern California, southern Arizona, Texas and Hawaii and has been naturalised in over 20 countries. Biological control is considered a viable long-term control option for S. terebinthifolia because release from natural enemies appears to be at least partly responsible for its success in Florida. We examined leaf phenology of S. terebinthifolia over a period of 15 months at five sites in central and southern Florida to provide information that may help in predicting the impacts of potential biocontrol agents for this weed. We documented leaf lifespan, the seasonality of leaf development and abscission and the survivorship of leaves that emerged during either spring, summer or autumn. Average leaf lifespan was >4.5 months at all sites, and leaf phenology followed the seasons closely. Although S. terebinthifolia possesses leaves throughout the year, leaf production was greatest from April to September, and most leaves were abscised in February and March. Spring- and summer-emerging leaves were also longer-lived than leaves produced during autumn. These results suggest that leaves of S. terebinthifolia would be most vulnerable to herbivory during the spring and summer months when newly growing leaf tissue is most plentiful. Biocontrol agents capable of damaging these tissues during spring/summer might be an effective means of controlling this invasive weed. 相似文献