Sorghum Pasta and Noodles: Technological and Nutritional Aspects

Plant Foods for Human Nutrition - Sorghum is a major cereal crop with various agronomic advantages, contains health-promoting compounds and is gluten-free. There is a growing tendency to use...     

Genetic bases of source-,sink-,and yield-related traits revealed by genome-wide association study in Xian rice

The source-sink relationship determines the ultimate grain yield.We investigated the genetic basis of the relationship between source and sink and yield potential in rice.In two environments,we identified quantitative trait loci(QTL)associated with sink capacity(total spikelet number per panicle and thousand-grain weight),source leaf(flag leaf length,flag leaf width and flag leaf area),source-sink relationship(total spikelet number to flag leaf area ratio)and yield-related traits(filled grain number per panicle,panicle number per plant,grain yield per plant,biomass per plant,and harvest index)by genome-wide association analysis using 272 Xian(indica)accessions.The panel showed substantial variation for all traits in the two environments and revealed complex phenotypic correlations.A total of 70 QTL influencing the 11 traits were identified using 469,377 high-quality SNP markers.Five QTL were detected consistently in four chromosomal regions in both environments.Five QTL clusters simultaneously affected source,sink,source–sink relationship,and grain yield traits,probably explaining the genetic basis of significant correlations of grain yield with source and sink traits.We selected 24 candidate genes in the four consistent QTL regions by identifying linkage disequilibrium(LD)blocks associated with significant SNPs and performing haplotype analysis.The genes included one cloned gene(NOG1)and three newly identified QTL(qHI6,qTGW7,and qFLA8).These results provide a theoretical basis for high-yield rice breeding by increasing and balancing source–sink relationships using marker-assisted selection.     

基于Meta分析评价秸秆覆盖对我国北方半干旱区不同生态区域小麦产量的影响

为了明确秸秆覆盖对我国北方半干旱区不同降雨量和积温条件下小麦产量的影响,利用Meta定量分析方法分析秸秆覆盖和不同秸秆覆盖量对我国北方半干旱区不同降雨量和积温条件下小麦产量的影响。以“半干旱区”、“秸秆覆盖”和“小麦产量”为关键词搜集、筛选并整理1970-2019年公开发表的相关文献,建立综合文献数据库。分析秸秆覆盖、不同秸秆覆盖量、不同年均降雨量和不同年均积温与小麦产量的关系。结果表明,秸秆覆盖可以增加北方半干旱区不同降雨量和积温区域的小麦产量,主要通过增加有效穗数使小麦平均增产12.77%;当秸秆覆盖量为6 000~9 000kg/hm ²时,小麦增产率最高,为14.49%（9.01%~20.52%,CI>95%）;当年均降雨量为200~400mm时,小麦增产率最高,为19.36%（9.86%~28.49%,CI>95%）;当年均积温为0℃~3 000℃时,小麦增产率最高,为19.56%（16.36%~22.39%,CI>95%）。此研究结果可为我国北方半干旱区不同降雨量和积温区域采用秸秆覆盖技术栽培小麦提供理论依据。     

Food animals as reservoirs and potential sources of multidrug-resistant diarrheagenic E. coli pathotypes: Focus on intensive pig farming in South Africa

BackgroundDiarrheagenic E. coli (DEC) strains are a major cause of diarrheal diseases in both developed and developing countries. Healthy asymptomatic animals may be reservoirs of zoonotic DEC, which may enter the food chain via the weak points in hygiene practices.AimWe investigated the prevalence of DEC along the pig production continuum from farm-to-fork.MethodsA total of 417 samples were collected from specific points along the pig production system, that is, farm, transport, abattoir and food. E. coli was isolated and enumerated using Colilert. Ten isolates from each Quanti-tray were selected randomly and phenotypically identified using eosin methylene blue agar selective media. Real-time polymerase chain reaction (PCR) was used to confirm the species and to classify them into the various diarrheagenic pathotypes. Antimicrobial susceptibility was determined against a panel of 20 antibiotics using the Kirby-Bauer disk diffusion method and EUCAST guideline.ResultsThe final sample size consisted of 1044 isolates, of which 45.40% (474/1044) were DEC and 73% (762/1044) were multidrug-resistant. Enteroinvasive E. coli (EIEC) was the most predominant DEC at all the sampling sites.ConclusionThe presence of DEC in food animal production environments and food of animal origin could serve as reservoirs for transmitting these bacteria to humans, especially in occupationally exposed workers and via food. Adherence to good hygienic practices along the pig production continuum is essential for mitigating the risk of transmission and infection, and ensuring food safety.     

成功治愈犬瘟热2例

犬瘟热是由犬瘟热病毒(CDV)引起的,是感染肉食兽中的犬科(尤其是幼犬),鼬科及一部分浣熊科动物的高度接触性、致死性传染病。病犬早期表现双相热型、急性鼻卡他,随后以支气管炎、卡他性肺炎、严重胃肠炎和神经症状为特征,少数病例出现鼻端和脚垫的高度角化。笔者在临床工作中,遇到过多例犬瘟热病例但仅有此2例成功治愈,现介绍如下:一、基本情况2006年4月18日晨,某犬场饲养员发现5头犬有咳嗽、厌食、流浓鼻涕等症状。兽医经过诊断,确诊其中一头藏獒(公,3月龄,10千克体重)为犬瘟热。4月19日晨,又有一头血犬(公,14月龄,35千克左右)发病:咳嗽…     

温度胁迫下沙葱萤叶甲小热激蛋白基因GdHsp20.6的克隆及表达分析

为明确小热激蛋白在沙葱萤叶甲Galeruca daurica应对温度胁迫中的作用,采用PCR方法克隆沙葱萤叶甲小热激蛋白基因Hsp20（GdHsp20.6）完整的开放阅读框（open reading frame,ORF）序列,应用在线软件对GdHsp20.6基因进行生物信息学分析,通过原核表达技术诱导表达及纯化其编码蛋白,并通过实时荧光定量PCR（real-time quantitative PCR,RT-qPCR）技术分析不同温度胁迫下GdHsp20.6基因的表达量。结果显示,GdHsp20.6基因ORF序列长度为543 bp,编码180个氨基酸,预测分子量为20.6 kD,无跨膜区和信号肽。GdHsp20.6氨基酸序列有高度保守的α-结构域。GdHsp20.6氨基酸序列与其它鞘翅目昆虫的Hsp20氨基酸序列有较高的一致性,其中与花绒寄甲Dastarcus helophoroides的Hsp20.99氨基酸序列的一致性最高,为63%。GdHsp20.6基因在大肠杆菌Escherichia coli BL21（DE3）细胞系中成功表达,经异丙基-β-D-硫代吡喃半乳糖苷（isopropyl-β-d-thiogalactoside,IPTG）诱导后GdHsp20.6蛋白成功表达,十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（sodium dodecyl sulfate-polyacrylamide gel electrophoresis,SDS-PAGE）和Western-blot分析表明融合蛋白大小与预测大小一致,并纯化获得了纯度较高的目的蛋白GdHsp20.6。低温（-10~5℃）和高温（35~40℃）处理1 h以及处理后25℃恢复30 min均能诱导GdHsp20.6基因表达上调,并且0℃处理30~120 min也能诱导GdHsp20.6基因表达上调。表明GdHsp20.6基因在沙葱萤叶甲应对低温和高温胁迫中可能起着重要作用。