Effects of insecticide acetamiprid on photosystem II (PSII) activity of Synechocystis sp. (FACHB-898)

Effects of insecticide acetamiprid on photosystem II (PSII) activity of Synechocystis sp. were investigated by a variety of in vivo chlorophyll fluorescence tests. Acetamiprid exposure increased the proportion of inactivated PSII reactive centers (PSII_X) and led to loss of active centers (PSII_A). High concentration (1.0 mM) acetamiprid decreased amplitude of the fast phase and increased the slow phase of fluorescence decay during reoxidation. The electron transport after Q_A was hindered by high concentration acetamiprid and more Q_A had to be reoxidized through S₂(Q_AQ_B)⁻ charge recombination. Acetamiprid decreased the density of the active reaction centers, electron transport flux per cross section and the performance of PSII activity but had little effect on dissipated energy flux per reaction center, antenna size and the maximum quantum yield for primary photochemistry (F_v/F_m). The target site of acetamiprid toxicity to the PSII of Synechocystis sp. was electron transfer on the acceptor side.     

在北京和河北局部地区Q型烟粉虱取代了B型烟粉虱

利用烟粉虱[Bemisia tabaci(Gennadius)]B生物型和Q生物型mtDNA COⅠ基因片段的特异性引物及烟粉虱mtDNA COⅠ基因片段通用引物,对2009年采自北京市海淀区、昌平区和河北廊坊市的蔬菜所3个基地的烟粉虱种群的生物型进行了鉴定,同时以本实验室在温室内长期饲养的B型烟粉虱种群作为对照。结果表明,2009年在3个地区所采集的烟粉虱全部为Q型,而在2004年这3个基地发生为害的烟粉虱全部为B型,表明Q型烟粉虱已经取代了B型烟粉虱在这些地区发生为害。     

传染性支气管炎病毒的鉴定

试验采用鸡胚接种和气管环培养相结合的方法,对IBV毒株进行培养鉴定,IBV经鸡胚传一代后再上鸡胚气管环培养可引起气管环纤毛运动停止,应用此法可以快速地进行IBV检测。     

新疆多浪羊淋巴细胞分离和培养

采集多浪羊的外周静脉血液,用人淋巴细胞分离液进行分离,收集分离得到淋巴细胞,同时经过纯化后通过镜检观察细胞形态。将纯化后的淋巴细胞在RPM I1640中置37℃,5%CO2培养箱4,6,8和12 h进行培养并观察淋巴细胞,其存活率分别为93.1%、92.4%、91.5%和90.6%。本试验成功地建立了多浪羊外周血淋巴细胞的分离技术和体外短期培养体系。     

北京地区8种无性系观赏型桧柏的植物学特性及水势特征

本研究对北京地区8个京桧无性系的5年生大田扦插苗的树形、冠径、叶形进行观测和对比分析,并测定了各无性系中植株的年均生长高度、新枝长度、小枝水势和土壤含水量,结果表明:不同无性系的植物学指标存在一定差异,其生境土壤含水量也有所不同;年均生长高度、新枝长度、冠径及小枝水势之间存在着不同的相关性.     

H1N1猪流感病毒血凝素蛋白抗原表位的筛选及其抗原性分析

本研究旨在对H1N1猪流感病毒血凝素蛋白抗原分子的模拟表位进行分离鉴定并分析其抗原性。用抗H1N1猪流感病毒血凝素蛋白小鼠血清IgG对噬菌体随机12肽库进行筛选,3轮亲和筛选后,特异性噬菌体得到了有效富集;对随机挑选的47个噬菌体克隆用ELISA进行鉴定,其中40个为阳性;对40个阳性克隆测序得到3种不同氨基酸序列;用Western blotting对获得的3个不同序列的噬菌体克隆进行抗原性分析,显示这3种噬菌体插入短肽能和H1N1猪流感病毒感染小鼠血清特异性结合。结果表明,本试验获得了3种H1N1猪流感病毒血凝素蛋白模拟抗原表位,它们都具有明显的抗原性,为H1N1猪流感病毒的疫苗研究和诊断奠定了基础。     

溶剂萃取法和固相微萃取法分析比较锦橙汁的香气成分

采用溶剂萃取法和固相微萃取法提取锦橙汁的香气物质。运用气相色谱—质谱对这些物质进行鉴定,其含量用相对峰面积表示。用正戊烷与乙醚的体积比为1∶2,萃取到37种化合物;采用固相微萃取法检测到53种化合物。上述2种方法共检测到8种相同的组分,其中酯类,烯类,醛类,醇类,酮类是主要的香气成分,烯类化合物是橙汁中含量最高的一类物质。     

日中性菊花及其分子育种前景

日中性菊花是指在适合菊花生长的正常温度范围内,在长日照或短日照条件下均能顺利进行花芽分化和花芽发育的菊花品种。本文在日中性菊花概念界定的基础上,对其品种选育、开花生理因素及遗传特性等方面的研究进行综述,阐明了植物日中性的形成机理是与光周期相关基因的异位表达导致下游成花基因FT表达量在不受日长影响下均能达到成花所需的阈值有关,并就日中性菊花的培育和分子育种研究的方向进行展望,为日中性菊花新品种培育和生产应用提供借鉴。     

浅谈如何加强园林绿化工程质量监督管理

园林绿化工程建设质量是项目建设的核心。如何通过程序化、技术化的管理模式,来增强工程质量管理水平,提高工程施工的质量,是加强绿化施工监督管理,规范绿化市场的关键。文章结合海口市绿化工程管理实际经验,从园林绿化工程建设质量管理、工程监理、施工管理、竣工验收等方面探讨加强园林绿化工程质量监督管理问题。     

Serological analysis of Chlamydophila abortus in Australian sheep and implications for the rejection of breeder sheep for export

Objective   To examine the incidence of positive results in a complement fixation test (CFT) and enzyme-linked immunosorbent assay (ELISA) for Chlamydophila abortus in Australian sheep and how this incidence differs with state of origin, age, sex, breed and property. To examine the consequences in relation to rejection of breeder sheep for export.
Design   Collection of blood samples from 891 sheep on 109 properties in southern Australia. All samples had a unique, coded property identification.
Procedure   The samples were tested using the Institut Pourquier Chlamydophila abortus antibody ELISA (rELISA) and a CFT. Residual maximum likelihood analyses of the sample to positive ratio of the corrected optical density for the rELISA and generalised linear mixed model analyses of the CFT outcomes were carried out.
Results   The sample to positive ratio of the corrected optical density values of the rELISA did not differ between sex, age, breed or state of origin, but differed greatly between properties. The CFT outcome did not differ between age, breed or state of origin, but differed greatly between properties and was more often positive with rams than with ewes.
Conclusion   Positive outcomes to C. abortus antibody tests are very common in Australia. Rams have a particularly high incidence of positive results with the CFT. Rejection of sheep and property consignments is likely to be very common with all tests and situations examined except for the CFT (at 1:32 dilution) in ewes.