芬苯达唑干混悬剂对绵羊裸头科绦虫的驱除效力试验

应用芬苯达唑干混悬剂,分别按5,10,20mg/kg体重剂量驱除绵羊裸头科绦虫,并设吡喹酮片剂20mg/kg体重剂量药物对照组和阳性对照组。粪检结果:芬苯达唑干混悬剂5,10,20mg/kg剂量对裸头科绦虫的粪便虫卵(节片)转阴率分别为70.0%、100.0%和100.0%。用药后14d剖检结果:芬苯达唑干混悬剂5mg/kg体重剂量对莫尼茨绦虫、无卵黄腺绦虫的驱虫率分别为70.7%、63.0%;10、20mg/kg体重剂量的驱虫率均达100.0%。结果表明10mg/kg和20mg/kg体重芬苯达唑干混悬剂试验剂量对绵羊裸头科2属绦虫均有效,其中10mg/kg以上剂量驱除绵羊裸头科绦虫高效安全。     

Novel Splice Variants of Bovine Interleukin-1 Receptor-Associated Kinase 2 （IRAK2）

Interleukin-1 receptor-associated kinases （IRAKs） are important signaling elements of the toll-like receptors family, which play a role in innate immune responses by coordinating host defense mechanisms. Presently different isoforms of human and murine IRAK2 molecules are cloned, but there is no report on the sequences and structure of bovine IRAK2 gene. In this study, we cloned the bovine IRAK2 gene by RT-PCR and RACE and discovered that there exist two alternative splicing of bovine IRAK2 genes, IRAK2a and 1RAK2b （GenBank accession no. EU528620 and EU528621）. IRAK2a gene is 2 148 bp coding 622 aa, which contains a death domain （aa 14-94） and a kinase domain （aa 205-440）, but 1RAK2b lacks 147 bp of exon 3 corresponding to 1RAK2a, and codes 386 aa which contains only partly kinase domain.     

柴达木绒山羊流产病调查及防治措施

 
柴达木绒山羊发生的母羊流产病影响了农牧民饲养绒山羊的积极性,一定程度上制约了柴达木绒山羊产业的发展。论文结合柴达木绒山羊流产病基本情况,分析造成柴达木绒山羊流产病的因素,并提出防治措施,供山羊养殖生产者参考。     

西藏垫状点地梅总生物碱提取工艺研究

[目的]研究垫状点地梅生物碱热回流提取的较优方法.[方法]采用热回流提取、单因素对比试验、正交试验和紫外-可见光分光光度分析,提出了垫状点地梅生物总碱的合理提取方案.[结果]乙醇体积分数75％、回流温度75℃、料液比（m/v） 1∶15、回流时间3h为最佳提取条件,此时提取垫状点地梅生物总碱提取率将达1.257％.[结论]该试验方法简便、科学可靠,可对垫状点地梅生物碱工业提取起到一定的参考价值.