Varying Nitrogen Concentrations to Optimize Basic Seed Potato Minitubers Production in a Three-Phase Hydroponic System

In hydroponics, nutrient management is the limiting factor to obtaining optimal production, and nitrogen (N) is the key component to consider when optimizing nutrient management in these types of systems. The objective of this study is to evaluate different combinations of N fertilizer concentrations in order to optimize the yield of basic seed potato minitubers in a three-phase hydroponic system. Treatments consisted of five combinations of N concentrations, applied before and after 21 days after plant transplant as follows, respectively: 1) 13 and 13, 2) 13 and 0, 3) 13 and 7.8, 4) 13 and 16, and 5) 13 and 26 mmol L^?1. Propagation was performed by transplanting 3–4 cm potato plantlets cv. Agata from sprouts. There were significant effects of N treatments on all measured variables (root, leaf, stem, and plant dry weight and minituber number and weight). To obtain the maximum minituber number yield, 9.51 minitubers/plant, corresponding to 67 minitubers/m², post 21-day adjusted N concentration was 18.4 mmol/L. Treatment 4 promoted higher basic seed potato minituber yield in a three-phase hydroponic system.     

Economic Optimum Nitrogen Fertilization Rates and Nitrogen Fertilization Rate Effects on Tuber Characteristics of Potato Cultivars

The objective of the research was to determine the economic optimum nitrogen (N) fertilization rates and to determine the effects of N fertilization rates on tuber characteristics and fresh mass loss after storage under cold and ambient conditions of four potato cultivars, Ágata, Asterix, Atlantic, and Monalisa. The experiments were, simultaneously, conducted in the same area and similar experimental conditions during the fall/winter period in Viçosa, Minas Gerais State, Brazil. In each experiment, five N fertilization rates (0, 50, 100, 200, 300 kg ha^?1) were evaluated in a randomized complete block design. For all cultivars, increasing N fertilization rate increased total and marketable yields and tuber dry matter yield up to a maximum following a quadratic model. Increasing N fertilization rate linearly increased the tuber protein concentration of Atlantic and Monalisa and had no effect on tuber pH. N fertilization rate effects on tuber mass, tuber titratable acidity, and fresh mass loss during storage were cultivar-dependent. N use efficiency (tuber yield divided by N fertilization rate) decreased with increase in N fertilization rate. The economic optimum N fertilization rates ranged from 147 to 201 kg ha^?1 depending upon cultivar and relative prices of N and potato tubers. Depending on the cultivar, under favorable price conditions (low N price and high tuber price), the economic optimum N fertilization rates to be applied by potato growers were 92–95% of the estimated N fertilization rate for obtaining the maximum potato yield, whereas under unfavorable conditions (high N price and low potato tuber price) the economic optimum N fertilization rates to be applied should be decreased to 86–92% of the rates for maximum yield.     

ORIGINAL ARTICLE: Effects of ambient temperature and soybean meal supplementation on intake and digestion of two sheep breeds differing in mature size

The aim of this study was to compare the intake and digestive physiology of mature ewes of two breeds ? Ile‐de‐France (mature weight: 75–80 kg) and Churra‐da‐Terra‐Quente (CTQ; mature weight: 45–50 kg) ? and evaluate the effects of ambient temperature and protein supplementation in the comparison. The temperature (25 °C vs. 11 °C) and soybean meal supplementation (150 g/kg of ingested hay on dry matter basis vs. unsupplemented control) were evaluated in 48 adult ewes of two breeds fed hay ad libitum and at a restricted level of intake. The intake, digestibility, rumen pH and NH₃–N, rumen outflow rates, faeces particle size and thyroid hormones levels were measured. These hormones can be related with gastrointestinal motility, thus explaining rumen outflow rate patterns. Dry matter intake per kg of body weight was higher in CTQ ewes (p < 0.05). This breed also exhibited lower organic matter and neutral detergent fibre digestibility (p < 0.001) and higher solid (p < 0.001) and liquid (p < 0.01) rumen outflow rates irrespective of intake level, supplementation or temperature. Rumen pH remained above 6.6 in all treatments. NH₃–N rumen content was similar (p > 0.05) when breeds were fed only hay. There was no breed effect (p > 0.05) on faeces particle size. Triiodothyronine was not affected (p > 0.05) by breed and thyroxine was higher (p < 0.10) in the CTQ breed but only at the lower temperatures (breed × temperature, p < 0.05). Ile‐de‐France sheep showed a lack of adaptation to lower temperatures. This study suggests that the native CTQ breed fulfils its metabolic needs by having a higher intake and inherits faster flow through the gastrointestinal tract, as a result, its digestive ability is diminished.     

A two-enzyme constituted mixture to improve the degradation of Arthrospira platensis microalga cell wall for monogastric diets

The main goal of this study was to test a rational combination of pre-selected carbohydrate-active enzymes (CAZymes) and sulphatases, individually or in combination, in order to evaluate its capacity to disrupt Arthrospira platensis cell wall, allowing the release of its valuable nutritional bioactive compounds. By the end, a two-enzyme constituted mixture (Mix), composed by a lysozyme and a α-amylase, was incubated with A. platensis suspension. The microalga cell wall disruption was evaluated through the amount of reducing sugars released from the cell wall complemented with the oligosaccharide profile by HPLC. An increase of the amount of reducing sugars up to 2.42 g/L in microalgae treated with the Mix relative to no treatment (p < .05), as well as a 7-fold increase of oligosaccharides amount (p < .001), were obtained. With resort of fluorescence microscopy, a 36% reduction of fluorescence intensity (p < .001) was observed using Calcofluor White staining. In the supernatant, the Mix caused a 1.34-fold increase in protein content (p = .018) relative to the control. Similarly, n-6 polyunsaturated fatty acids (PUFA) (p = .007), in particular 18:2n-6 (p = .016), monounsaturated fatty acids (MUFA) (p = .049) and chlorophyll a (p = .025) contents were higher in the supernatant of microalgae treated with the enzyme mixture in relation to the control. Taken together, these results point towards the disclosure of a novel two-enzyme mixture able to partial degrade A. platensis cell wall, improving its nutrients bioavailability for monogastric diets with the cost-effective advantage use of microalgae in animal feed industry.     

X-ray imaging of shock waves generated by high-pressure fuel sprays

Synchrotron x-radiography and a fast x-ray detector were used to record the time evolution of the transient fuel sprays from a high-pressure injector. A succession of 5.1-microsecond radiographs captured the propagation of the spray-induced shock waves in a gaseous medium and revealed the complex nature of the spray hydrodynamics. The monochromatic x-radiographs also allow quantitative analysis of the shock waves that has been difficult if not impossible with optical imaging. Under injection conditions similar to those found in operating engines, the fuel jets can exceed supersonic speeds and result in gaseous shock waves.     

Linking fish ecomorphotypes to food categories and local-scale habitat uses along a Brazilian coastal stream

Fish ecomorphological analyses often deal with several traits simultaneously, making it challenging to interpret general patterns, and have not addressed the longitudinal component of streams in morphology-habitat relationships. We identified ecomorphotypes of fishes and correlated their morphological variation with food, structural/hydrological variables, and abundances to detect their links with diet and local-scale habitat categories. Eighteen morphometric traits were obtained from specimens collected by electrofishing in a coastal Atlantic stream in Brazil. To test for morphology-habitat associations, headwaters, middle, and mouth reaches were divided into shorter sampling sites, with the middle reach being classified into riffle, run, and pool mesohabitats. Multivariate analyses highlighted the morphological variations and associated the form categories of 18 fish species with food and habitat categories. It resulted in four combined trophic and habitat ecomorphotypes: (1) benthic AB/lithophilic, composed of smaller to longer loricariids, detritivores, of fast-water, and pebbly habitats; (2) benthic C/lithophilic of very fast-waters, algal and detritus scrapers, with longer intestines than the other benthic species, wider suctorial mouths, inhabitants of headwaters, and riffles habitats; (3) nektobenthic/lithophilic with streamlined bodies, invertebrate feeders, and dwellers of faster-water, cobbly habitats; and (4) nektonic/limnophilic composed mainly by characins, which had strong correlations with terrestrial insect consumption, and lentic/pool, sandy habitats. The morphology–environment correlations linked these ecomorphotypes to the broad habitat gradient arrayed in longitudinal and local scales. These results permit inferring the larger form patterns expected for local fish assemblages and emphasise the fish trait categorisation as a possible surrogate to reveal broader ecomorphological associations.