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排序方式: 共有125条查询结果,搜索用时 15 毫秒
121.
de Jesús Pires Carolline Costa Marcones Ferreira Zucchi Maria Imaculada Ferreira-Gomes Regina Lucia Pinheiro José Baldin Viana João Paulo Gomes Bajay Miklos Maximiliano Assunção-Filho José Ribamar de Almeida Lopes Ângela Celis 《Genetic Resources and Crop Evolution》2022,69(3):973-986
Genetic Resources and Crop Evolution - Lima bean (Phaseolus lunatus L.) have high genetic diversity, especially in Brazil, which has been maintained both on farms and in germplasm banks. Knowledge... 相似文献
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Carla Marassi Carlos Almeida Sonia Pinheiro Silvio Vasconcellos Walter Lilenbaum 《Veterinary research communications》2009,33(8):937-943
After one clinical case that evidenced the outbreak, a complete screening by intradermal tuberculin test was performed in
one goat herd in Brazil. The herd was composed by 500 animals and 83 of them (16.6%) showed to be reactive to the comparative
double cervical intradermal test. Four months after the test, all the 83 reactive animals were slaughtered and blood samples
were collected from 45 of them, for serological assays. From those 45, 32 were randomly chosen for necropsy and histopathological
and bacteriological procedures were conducted. Histopathology evidenced at least one characteristic lesion of tuberculosis
in each animal, with typical granulommas where acid-fast bacilli (AFB) could be observed. Bacteriology was positive for Mycobacterium bovis in 22 samples (68.7%), therefore confirming the etiology of the outbreak. Sera of 45 animals plus 20 other from a certified
free tuberculosis farm were tested in an ELISA using the recombinant M.bovis protein MPB70 as capture antigens. From those, 43 were reactive to the test, with high ODs results, considering a cut-off
point established by ROC curve analyzing results (cut-off = 0.8; mean = 0.55; range: 0.157–1.357). These results suggest that
MPB70-ELISA can be considered as a reliable tool to diagnose tuberculosis in goat herds, since this assay was capable to correctly
detect 95.6% of the animals here examined. 相似文献
125.
BC Schimming CAE Baumam PFF Pinheiro R de Matteis RF Domeniconi 《Reproduction in domestic animals》2017,52(4):617-624
Aquaporins (AQPs) are channel proteins that facilitate the transepithelial and bidirectional movement of water. AQP9 is an aquaporin that is expressed in the mammalian epididymis. This water transport contributes to epididymal sperm concentration. This study aimed to examine the morphology of epididymal epithelium in piglets and boars, as well as the expression and immunolocalization of AQP9. The piglets presented an epididymal epithelium in differentiation with principal, basal and apical cells. The cellular population of the epididymal epithelium in boars consisted of principal, basal, apical, clear and narrow cells. The migratory cells known as halo cells were observed in the epididymis of both piglets and boars. AQP9 expression presented differences between piglets and boars. Moderate intensity of AQP9 immunoreaction was observed in the apical border of the epididymal epithelium of the caput and cauda regions in the piglet epididymis. A moderate‐to‐intense reaction for AQP9 was observed in the nuclei of epithelial cells of the three epididymal regions in the boar epididymis. The region of the cauda epididymis showed reactivity for AQP9 also in the apical border of the epithelium. It is believed that the AQP9 is already functional in piglets at only 1 week of age and is more active, playing a pivotal role in the caput and cauda regions of the epididymis. Moreover, the intense AQP9 expression in the apical border of epithelial cells in the cauda region of the boar epididymis suggests a higher performance of AQP9 in this region, where sperm complete their maturation process, stored and concentrated. 相似文献