Nutritional evaluation and productivity of supplemented sheep grazing in semiarid rangeland of northeastern Brazil

Tropical Animal Health and Production - Sheep production systems in Brazilian caatinga rangelands require supplementation adapted to changes in floristic and chemical composition as dry seasons...     

Effects of Feeding Levels on Growth Response,Body Composition,and Energy Expenditure in Blackspot Seabream,Pagellus bogaraveo,Juveniles

Although reported as a slow growing species, to date information regarding nutritional dynamics of blackspot seabream, Pagellus bogaraveo, is limited, especially during ongrowing phase. This study determined the effects of different feeding levels on growth response, body composition, and energy expenditure of blackspot seabream juveniles (initial weight = 64 g). Fish were allocated in nine indoor tanks (15 fish per tank) and fed a basal diet twice daily at increasing levels (2.5, 3.3, and 4.1 g feed/kg^0.8/d) for 138 d. Growth response decreased with decreasing feeding levels, but only at 2.5 g feed/kg^0.8/d (P < 0.001). Differences in growth rates were positively correlated with body lipid content (r² = 0.90). Net protein utilization decreased with each increase in feeding level. The energy requirement for zero growth (maintenance) and the estimated energy loss on starvation were calculated as 22.6 and 17.9 kJ/kg^0.8/d, respectively. The energy cost for protein and lipid gain was calculated as 3.8 and 1.4 kJ/kJ energy deposited, respectively. This study suggested that blackspot seabream may grow slower than the other close‐related species because of its higher demand of dietary protein energy in favor of lipid deposition.     

Lippia alba (Verbenaceae) hydrolate as sedative of tambaqui (Colossoma macropomum) juveniles in simulated transport conditions

Lippia alba hydrolate (LAH) is produced as a secondary product in essential oil extraction. The objective of this study was to evaluate the use of LAH in a simulation of the transport of tambaqui (Colossoma macropomum) juveniles. A series of 17 hr simulations were performed using load densities of 30, 60 and 90 juveniles per L and four LAH concentrations of 0.4%, 1%, 2% and 5%, with a control of just water. Fish survival, temperature, dissolved oxygen, conductivity, pH, ammonia excretion, alkalinity water hardness and net Na⁺, K⁺, Cl^‐ fluxes, were all evaluated before and after the transport simulations. Mucous cell densities in the gills were also determined microscopically, post simulation. The results showed that addition of 5% LAH achieved better survival during the simulation than the water control. Dissolved oxygen was significantly lower with an increased load density. Also, conductivity, NH₄ excretion and net ionic fluxes (K⁺ and Cl^?) were lower with 5% LAH, although mainly where higher load densities were tested. Mucous cell density was lower in LAH concentrations of 2% and 5%, again with higher load densities. In view of these findings, it is suggested that 5% LAH is an effective sedative for use in the transport of tambaqui, mainly with high load densities. This work is the first study of LAH and highlights its potential applications in aquaculture management.     

Fish anesthesia: effects of the essential oils of Hesperozygis ringens and Lippia alba on the biochemistry and physiology of silver catfish (Rhamdia quelen)

The anesthetic activities of the essential oils (EOs) of Hesperozygis ringens (EOHR) and Lippia alba (EOLA) and their effects in silver catfish (Rhamdia quelen) after anesthesia and recovery were investigated. Fish (32.19 ± 1.24 g) were submitted to one of the following treatments for each EO: basal group, control, or anesthesia (150, 300, or 450 μL L^?1 EO). After that the anesthesia was induced or simulated and the biometric measurements were completed, fish were transferred to anesthetic-free aquaria to allow for recovery. Fish were sampled at 0, 15, 30, 60, and 240 min after recovery. At time 0 of recovery, the ventilatory rate was lower in the groups anesthetized with either EO. In comparison with the basal group, control fish showed an increase in plasma glucose, aspartate aminotransferase (AST), and Na⁺ levels and a reduction in Na⁺/K⁺-ATPase activity at 0 min of recovery. Plasma levels of ammonia and Na⁺ were lower in the fish anesthetized with EOLA (450 μL L^?1) and EOHR (all concentrations), respectively, than in the control fish. Additionally, lactate, AST, alanine aminotransferase, K⁺ plasma levels, and gill Na⁺/K⁺-ATPase and H⁺-ATPase activities were higher in the fish anesthetized with either EOHR or EOLA than in the control fish. The EOs promoted slight changes in silver catfish that enabled both an adaptive response and the recovery of most of the measured parameters after 240 min regardless of concentration or EO that was used. These findings support the use of EOHR and EOLA as anesthetics for fish.     

Ammonia,Nitrous Oxide,Carbon Dioxide and Methane Emissions from Commercial Broiler Houses in Mediterranean Portugal

Limited data are available on ammonia (NH₃), nitrous oxide (N₂O), carbon dioxide (CO₂) and methane (CH₄) emissions from poultry housing in Mediterranean countries. The aim of the present study was to assess the NH₃, N₂O, CO₂ and CH₄ emission rates from commercial breeding hen and broiler houses under Mediterranean climate conditions. Research was conducted at one commercial breeding hen house and in two commercial broiler houses located in central Portugal. The environmental conditions, gas concentrations and ventilation rates were measured in the cold (8.0?±?2.1 °C) and hot (20.7?±?1.9 °C) season for the breeding hen house, whereas for the two broiler houses, measurements were made during one fattening cycle in the fall (17.3?±?1.7 °C) season. Results showed that the annual average emission rates for breeding hen and broiler houses were 0.52?±?0.27 and 0.06?±?0.01 for NH₃, 0.030?±?0.042 and 0.006?±?0.001 for N₂O, 169.6?±?56.2 and 58.0?±?15.1 for CO₂ and 0.092?±?0.131 and 0.0113?±?0.0002 g day^?1 bird^?1 for CH₄, respectively. The N₂O emission rates observed in breeding hen houses may have been overestimated, being higher than previously reported for Mediterranean countries.     

Estimation of natural outcrossing rate and genetic diversity in Lima bean (<Emphasis Type="Italic">Phaseolus lunatus</Emphasis> L. var. <Emphasis Type="Italic">lunatus</Emphasis>) from Brazil using SSR markers: implications for conservation and breeding

Lima bean (Phaseolus lunatus L.) is an important food source in Brazil, especially in the northeast region, where its production and consumption are high. The goals of the present study were to estimate natural outcrossing rates and genetic diversity levels of Lima bean from Brazil, using ten microsatellite loci to obtain information for their conservation and breeding. Fourteen accessions were selected from an experiment in field with open-pollinated and with the presence of pollinating insects. Twelve seeds of each of the 14 selected accessions were grown in screenhouse for tissue harvest and DNA extraction. The multilocus model was used to determine the reproductive system. The outcrossing rate was 38.1 % (t_m = 0.381; t_s = 0.078), and the results indicated a mixed mating system with a predominance of selfing (1 ? t_m = 61.9 %). The biparental inbreeding rate was high (t _m  ? t _s  = 0.303) and the multilocus correlated paternity was quite high (r _p(m) = 0.889), indicating that the progeny was mostly composed of full sibs. The average effective number of pollen donors per maternal plant (N _ep ) was low (1.12), and the fixation index for maternal genotypes (F _m ) was 0.945, indicating that most genitors resulted from inbreeding. The studied families presented considerable genetic variability: A = 6.10;  %P = 30; H _e  = 0.60 and H _o  = 0.077. Total diversity was high (H _T = 0.596), and a portion was distributed within families (H _S = 0.058). In addition, diversity was higher between families (D _ST = 0.538), and genetic differentiation was high (G _ST = 0.902). The results presented here can be used in the implementation of Lima bean conservation and breeding programs in Brazil.     

Economic viability of Phaseolus vulgaris (BRS Estilo) production in irrigated system in a function of application of leaf boron

Foliar fertilization may be a viable strategy to boron supply in irrigated cropping systems with common beans (Phaseolus vulgaris), since it prevent B leaching. The aim of this work was to evaluate the economic viability and physiological parameters of the common beans production in irrigated cropping systems using sources and increasing foliar boron doses. A field experiment was carried out using an experimental block design in a factorial scheme 2?×?5?×?3, with two sources of B (boric acid and borax) and five doses: 0 (control), 2, 4, 6 e 8?kg?ha^?1, with three repetitions. Foliar B applications were performed at 40 days after seeds germination, in pre-flowering stage. Physiological process (transpiration, stomatal conductance, CO₂ internal concentration, net photosynthesis, and relative chlorophyll index), B level in leaves and grain yield were measured. These data were used to determine the economic viability of B fertilization in common beans. Both boric acid and borax increased B levels in common beans leaves. Borax affected some physiological process reducing stomatal conductance and increasing net photosynthesis. Using borax, the highest net photosynthesis was observed at a rate of 4?kg?ha^?1, while the boric acid increased net photosynthesis linearly after increasing B doses application. An enhance of 311?kg?ha^?1 in the grain yield was observed using borax related to the control (without B application); however, grain yield decreased linearly after application of increasing B doses, as boric acid. Comparing the economic viability of sources and doses of B, the highest profitability is obtained using borax at a rate of 4?kg?ha^?1, which promoting a differential profit of US$534.44 per hectare compared to common beans cropping without B.