Epiglottic Augmentation in the Horse

Epiglottic augmentation with injectable bovine collagen or an autogenous or allogenous auricular cartilage graft was performed in 12 horses with endoscopically and radiographically normal epiglottises. The grafting procedures were easy to perform and did not cause apparent discomfort. Cartilage graft extrusion or resorption may have occurred, but was not seen by endoscopy and lateral laryngeal radiography. Only collagen implants remained evident endoscopically, as smooth round submucosal bulges ventral to the epiglottic cartilage. Two horses with collagen implants, and all horses with cartilage autografts and allografts, were euthanatized at week 16. One horse with a collagen implant was euthanatized at week 4 and one at week 6. The epiglottis appeared thickened in three horses with collagen implants, two horses with autogenous grafts, and three horses with allogenous grafts. Pharyngeal lymphoid tissue was hyperplastic in two horses with autografts and three horses with allografts, but not in horses with collagen implants. Collagen grafts persisted as one or two smooth bulges 8 mm in diameter. Collagen incited a brisk foreign body reaction that was surrounded by a fibrous connective tissue capsule. Epiglottises of the horses with collagen implants were significantly thicker 20 mm from the tip than those of normal horses and horses with allografts. Cartilage graft incorporation was not evident grossly and was seen on microscopic examination in only one autograft. Thickening was caused by submucosal fibrosis.     

Feeding success and survivorship of Arctic cod larvae,Boreogadus saida,in the Northeast Water polynya (Greenland Sea)*

We tested the hypothesis that enhanced feeding success and survivorship of Arctic cod larvae in the Northeast Water polynya (Greenland Sea) are associated with high densities of their zooplankton prey in areas of low sea ice concentration. From late May to early August 1993, first-feeding yolk-sac larvae (6.5–8.5 mm long) were collected primarily along the coast and over shallow banks in the polynya, whereas post yolk-sac larvae (8.5–14 mm) were more uniformly dispersed over the sampling area. Arctic cod larvae < 14 mm fed almost exclusively on copepod nauplii (74% of total prey number) and copepod eggs (16%). Feeding success was defined as the residuals of the regression of the number of prey ingested against larval length. Nauplii density varied from 9600 to 731300 nauplii m^-2(equivalent to 0.16 to 12.2 nauplii 1^-1) but, contrary to our hypothesis, seldom limited the feeding success of Arctic cod larvae. Water temperature was the best predictor of feeding success in larvae of all sizes. First-feeding success and survivorship were low for larvae hatched before mid-July, and improved thereafter as surface temperatures increased in the open waters of the polynya. There was no relationship between feeding success and irradiance or wind. Assuming a fixed spawning season, it is concluded that a larger fraction of the newly hatched larvae would experience adequate temperatures in years when the polynya opens early. Based on this conclusion, we propose that the timing of the opening of polynyas is a critical determinant of year- class strength in Arctic cod, a key species in the Arctic food web.     

Occurrence of an exceptional catch of pelagic juvenile lingcod (Ophiodon elongatus) off Point Reyes, California

An exceptionally large catch of pelagic juvenile lingcod (Ophiodon elongatus) was made just offshore of Point Reyes, California in 1990. Results of 678 midwater trawls from annual surveys from 1983 to 1990 show that pelagic juvenile lingcod have a strong nearshore distribution and were most abundant in the Gulf of the Farallones, with a mean abundance of 5.0 per trawl. Otoliths from fish taken in the large catch were used to estimate parameters of a log-transformed Gompertz growth model, which was then used to calculate hatch dates. Hatching peaked around 24 March and ranged from 10 March to 19 April, implying a January-February spawning season for lingcod off central California. The large catch was associated with an anticyc-lonic eddy which would lead to water being trapped near the coast; convergence of fish associated with the eddy may have been due to either physical or biological effects.     

Chemonucleolysis with Collagenase A Radiographic and Pathologic Study in Dogs

Collagenase, a proteolytic enzyme, was injected intradiscally in nine clinically normal, middle-aged beagles. Calcium chloride diluent solution (control), 100 ABC units of collagenase, and 250 ABC units of collagenase, were injected in randomly selected intervertebral discs (T13-L1 to L5-L6). On day 11, the discs injected with collagenase were narrowed radiographically, but there was no significant change in myelograms. Grossly and histologically, there was dissolution of the intervertebral discs, mainly nucleus pulposus, and protrusion of nucleus material in the vertebral body through bony end-plates in discs injected with collagenase. Collagenase chemonucleolysis may be an alternative to spinal surgery for intervertebral disc protrusion in dogs.