全文获取类型
收费全文 | 184篇 |
免费 | 22篇 |
专业分类
林业 | 11篇 |
农学 | 6篇 |
36篇 | |
综合类 | 4篇 |
农作物 | 17篇 |
水产渔业 | 16篇 |
畜牧兽医 | 93篇 |
园艺 | 4篇 |
植物保护 | 19篇 |
出版年
2024年 | 1篇 |
2023年 | 5篇 |
2022年 | 5篇 |
2021年 | 9篇 |
2020年 | 12篇 |
2019年 | 13篇 |
2018年 | 9篇 |
2017年 | 15篇 |
2016年 | 14篇 |
2015年 | 10篇 |
2014年 | 8篇 |
2013年 | 17篇 |
2012年 | 14篇 |
2011年 | 10篇 |
2010年 | 3篇 |
2009年 | 7篇 |
2008年 | 10篇 |
2007年 | 3篇 |
2006年 | 8篇 |
2005年 | 5篇 |
2004年 | 2篇 |
2003年 | 5篇 |
2002年 | 5篇 |
2001年 | 3篇 |
2000年 | 7篇 |
1999年 | 2篇 |
1997年 | 2篇 |
1992年 | 1篇 |
1991年 | 1篇 |
排序方式: 共有206条查询结果,搜索用时 15 毫秒
41.
Shannon A. Kenny Matthew R. Cook Jennifer A. Lenz Karl C. Maritato Katherine A. Skorupski Brandan G. Wustefeld-Janssens MacKenzie A. Pellin Catrina J. Silveira Stan Veytsman Laura E. Selmic Brian D. Husbands 《Veterinary and comparative oncology》2023,21(4):587-594
Renal carcinomas (RC) are uncommonly encountered in feline medicine. Limited information regarding clinical presentation and postoperative outcomes is available. The purpose of this multi-institutional, retrospective study was to describe the presenting features and clinical outcomes of cats with RC undergoing nephrectomy. Thirty-six client-owned cats were included. Medical records from participating institutions were searched to identify cats that had a histopathologic diagnosis of RC and underwent nephrectomy from January 2001 to October 2021. The most common presenting complaints were weight loss (36.1%) and hyporexia (30.6%). Based on preoperative imaging and intraoperative findings, eight cats had suspected metastasis at the time of surgery (22.2%). Twenty-eight cats survived to discharge (77.8%). Median progression free interval (PFI) could not be determined, as only six cats developed suspected recurrence (16.7%) and seven cats developed suspected metastasis (19.4%). The all-cause median survival time (MST) was 203 days (95% confidence interval [CI]: 84, 1379 days). When cases that died prior to discharge were excluded, MST increased to 1217 days (95% CI: 127, 1641 days). One-year, two-year, and three-year survival rates were all 40.4%. Neither renal tumour histologic subtype nor the presence of preoperative azotemia, anaemia, erythrocytosis, haematuria, or suspected metastasis at diagnosis were found to influence survival. For cats surviving to discharge, prolonged survival times were possible. Further studies are necessary to elucidate other potential prognostic factors, the utility of postoperative adjuvant treatment, and to identify cats at-risk of mortality in the perioperative period. 相似文献
42.
O A Rocha A F Bicalho J N Silveira E S Lopes I F Heneine 《DTW. Deutsche tier?rztliche Wochenschrift》1992,99(4):143-145
Bothrops jararaca venom was toxoided by stepwise iodination with cold iodine, and doses up to 30 LD50 were non-lethal by i.p. route (BICALHO et al., 1990). Groups of mice injected chronically with the native, or the iodinated venom, have been subjected to histological examination. In the native group, in the spleen, around the white pulp, an acellular, amorphous eosinophilic substance, metachromatic to Methyl Violet, PAS positive, and dichroic to Congo Red under polarized light, was present. Strong congestion in the liver, kidneys and lungs was found. The salivary glands were replenished with an amorphous substance in the serosal acini. The groups injected with the iodinated venom only show discrete alterations, more akin to the control group. The anavenin was immunogenic. Antibody generation in mice and rabbits was detected by ELISA. In mice, active protection against challenge with native venom was presented. The iodinated venom generated a rabbit antiserum with strong lines in gel immunoprecipitation against the lethal venom. A minimum neutralization titer of 2.3 mg ml-1 against the native venom was attained in the first cycle (28 days) of immunization. After 3 cycles (100 days), the protection rose to 5.1 mg ml-1. 相似文献
43.
Eliana Guedes Stehling Tatiana Amabile Campos Marcelo Brocchi Vasco Ariston de Carvalho Azevedo Wanderley Dias da Silveira 《Journal of veterinary science (Suw?n-si, Korea)》2008,9(1):75-83
An Escherichia coli strain (SEPT13) isolated from the liver of a hen presenting clinical signs of septicaemia had a LD50 of 4.0 × 105 CFU/ml in one-day-old chickens, expressed Ia, Ib, E1, E3, K and B colicins and aerobactin. The strain was ampicillin and streptomycin resistant, and found to have fimA, csgA and tsh DNA related sequences; it could adhere to and invade HEp-2 and tracheal epithelial cells, expressed fimbriae (observed by electron microscopy), and had five plasmids of 2.7, 4.7, 43, 56, and 88 MDa. Transposon mutagenesis of strain SEPT13, with transposon TnphoA, resulted in a mutant strain named ST16 that had a LD50 of 1.2 × 1012 CFU/ml. All other biological characteristics of strain ST16 were the same as those detected for strain SEPT13 except for the migration of an 88 MDa plasmid to the 93 MDa position indicating the insertion of the transposon into the 88 MDa plasmid. The 93 MDa plasmid of strain ST16 was transferred, by electroporation assay, to non-pathogenic receptor strains (E. coli strains K12 MS101 and HB101), resulting in transformant strains A and B, respectively. These strains exhibited adhesion properties to in vitro cultivated HEp-2 cells but did not have the capacity for invasion. The adherence occurred despite the absence of fimbriae; this finding suggests that the 88 MDa plasmid has afimbrial adhesin genes. 相似文献
44.
Fbio S. Mendona Ricardo Z. Vaz Fabiano N. Vaz Willian S. Leal Isabella D. B. Silveira Joo Restle Arione A. Boligon Fernando F. Cardoso 《Animal Science Journal》2019,90(2):288-296
This study aimed to assess preslaughter factors responsible for the occurrence of bruises to front, rib, loin, hip, and round sites of cattle carcasses. Data from the slaughter of 148 loads of cattle were assessed, totaling 4,611 carcasses. Evaluated bruising factor variables were animal gender, journey distance (km), vehicle type (m²), journey time (hours), load density (kg/m2), handling and facilities conditions (optimal, acceptable, and unacceptable), and unloading waiting time (min). Gender had the strongest influence on carcass bruising, being the first variable to enter in the majority of models related to the carcass sites. Female carcasses displayed higher bruising in all sites. Densities above 401 kg/m² caused more bruising. With the exception of the loin, unacceptable, and acceptable conditions assigned to farm facilities and handling resulted in higher average bruising. Articulated vehicles with a greater load capacity were more likely to cause bruising. The effects of variables causing bruises act differently in the various carcass sites. In this sense, the results of this study suggested possible management practices for specific problems during the preslaughter period to minimize losses by bruising to different carcass sites. 相似文献
45.
Denise S. Okano Jurandy M. Penitente‐Filho Victor E. Gomez Len Paula P. Maitan Camila O. Silveira Bruna Waddington Edgar A. Díaz‐Miranda Eduardo P. da Costa Simone E. F. Guimares Jos D. Guimares 《Reproduction in domestic animals》2019,54(3):604-612
This study aimed to assess characteristics of bovine cryopreserved sperm and evaluate its relation to field fertility in fixed‐time artificial insemination (FTAI). Semen samples of 16 bulls were used to inseminate 811 Nellore cows, and four of these bulls were also used to inseminate 101 Nellore heifers. Samples of the same ejaculate used for FTAI from each bull were analysed in the laboratory after thawing. Sperm motility and vigour were subjectively assessed by light microscope, and integrity of the plasma and acrosome membranes, and H2O2 production were evaluated by flow cytometer. Relation among sperm characteristics and pregnancy rate of cows and heifers were evaluated by univariate and multivariate logistic regression. Subjective sperm motility and vigour did not affect the probability of pregnancy in cows or heifers. In univariate analysis for pregnancy in cows, sperm traits related to acrosome injury positively affected probability of pregnancy mainly when associated with plasma membrane integrity; H2O2 production seems to be less important than plasma membrane integrity in affecting probability of pregnancy. In multivariate analysis, sperm traits related to injured acrosome positively affected probability of cow and heifer pregnancies while intact acrosome was negatively related to cow pregnancy. Intact plasma membrane and high H2O2 production were positively related to cow pregnancy but negatively related to heifer pregnancy. Results suggest that a capacitation‐like status of the acrosome may benefit probability of pregnancy in cows. 相似文献
46.
Fábio Raphael Pascoti Bruhn Guilherme Loureiro Werneck David Soeiro Barbosa Daniel Cardoso Portela Câmara Taynãna César Simões Lia Puppim Buzanovsky Anna Gabryela Sousa Duarte Saulo Nascimento de Melo Diogo Tavares Cardoso Lucas Edel Donato Ana Nilce Silveira Maia-Elkhoury Vinícius Silva Belo 《Zoonoses and public health》2024,71(2):144-156
47.
Cicera Raquel Fernandes Rodrigues Evandro Nascimento Silva Sérgio Luiz Ferreira‐Silva Eduardo Luiz Voigt Ricardo Almeida Viégas Joaquim Albenisio Gomes Silveira 《植物养料与土壤学杂志》2013,176(2):157-164
This study assessed the relationships between external K+ supply and K+ : Na+ ratios associated with Na+ toxicity in Jatropha curcas. Plants were exposed to increasing external K+ concentrations (6.25, 12.5, 25, 37.5, and 50 mM), combined with 50 mM NaCl in a nutrient solution. Photosynthesis progressively increased as the external K+ : Na+ ratios increased up to 0.75. The increase of photosynthesis and plant dry matter correlated positively with K+ : Na+ in xylem and leaves. The transport rates of K+ and Na+ from roots to xylem and leaves were inversely correlated. These ions presented an antagonistic pattern of accumulation in all organs. Maximum rates of photosynthesis and plant growth occurred with leaf K+ : Na+ ratios that ranged from 1.0 to 2.0, indicating that this parameter in leaves might be a good indicator for a favorable K+ homeostasis under salinity conditions. The higher K+ affinity and selectivity compared with Na+ in all organs associated with higher xylem flux and transport to shoots are essential for maintaining adequate K+ : Na+ ratios at the whole‐plant level. These characteristics, combined with adequate K+ concentrations, allow J. curcas to sustain high rates of photosynthesis and growth even under toxic NaCl levels. 相似文献
48.
Maria Izabel Gomes Silva Maria Anglica Gomes Silva Manuel Rufino de Aquino Neto Brinell Arcanjo Moura Helenira Loureno de Sousa Everton Paulo Homem de Lavor Patrícia Freire de Vasconcelos Danielle Silveira Macêdo Damio Pergentino de Sousa Silvnia Maria Mendes Vasconcelos Francisca Cla Floreno de Sousa 《Fitoterapia》2009,80(8):506-513
The present study investigated the effects of isopulegol, a monoterpene alcohol, in PTZ-induced convulsions and verified possible involved mechanisms. Saline, isopulegol or diazepam were intraperitonealy injected 30 min before PTZ. The latency for development of convulsions and mortality, as well as the mortality protection percentage was recorded. For investigating the involvement of GABAergic system, flumazenil was utilized. The activity of antioxidant enzyme catalase as well as the levels of reduced glutathione and lipid peroxidation were measured in brain hippocampus. Similarly to diazepam, isopulegol significantly prolonged the latency for convulsions and mortality of mice. All animals were protected against mortality at higher dose of isopulegol. Flumazenil pretreatment decreased the prolongation of seizure latency induced by both diazepam and isopulegol, although it was not able to reverse the latency and protection percent for mortality. Isopulegol also significantly prevented PTZ-induced increase in lipid peroxidation, preserved catalase activity in normal levels, and prevented the PTZ-induced loss of GSH in hippocampus of mice. These results suggest that the anticonvulsant and bioprotective effects of isopulegol against PTZ-induced convulsions are possibly related to positive modulation of benzodiazepine-sensitive GABAA receptors and to antioxidant properties. 相似文献
49.
Gary W. Webb PhD PAS Codi L. Burris MS Sarah E. Harmon MS Rachel H. Baker MS 《Journal of Equine Veterinary Science》2011,31(4):166-173
Three experiments were conducted to determine whether replacement of chicken egg yolk, as a component of freezing extenders, with egg yolk from other avian species would improve the post-thaw motility and percentage of intact acrosomes of stallion spermatozoa. In the first experiment, substitution of chicken egg yolk with chukar egg yolk, as a component of the lactose-ethylenediaminetetraacetic acid extender, improved (P ≤ .05) the post-thaw motility of stallion spermatozoa. These results were not replicated in (IMV Technologies, Maple Grove, MN, USA) a more expansive study comparing 2%, 4%, 6%, or 8% egg yolk combined with INRA 96 when a “slow freeze” method was used, or the same substitution at levels ranging from 13% to 22% when egg yolk was combined with lactose-ethylenediaminetetraacetic acid for diluents used for a “fast freeze” method of cryopreservation. In the third study, egg yolks from regular and high omega-3 chicken eggs as well as from turkey, chukar, and mallard duck eggs were analyzed for lipid content and fatty acid profile. The yolk from the turkey eggs was higher (1,300 mg/100 g) and that from mallard ducks was lower (560 mg/100 g) in cholesterol as compared with the two types of chicken eggs and chukar egg yolk (range, 1,046-1,094 mg/100 g). In addition, the high omega-3 eggs did test higher for fatty acids (4.51 g/100 g) than other types of eggs (range, 0.28-0.73 g/100 g). Substitution of chicken egg yolk with turkey, but not duck, egg yolk resulted in higher post-thaw total motility (P ≤ .05) for spermatozoa obtained from two of the three stallions used in the third experiment. 相似文献
50.
Risely Ferraz De Almeida Camila Haddad Silveira Raquel P. Mota Mara Moitinho Everton Martins Arruda Eduardo De Sá Mendonça Newton La Scala Beno Wendling 《Journal of Soils and Sediments》2016,16(10):2354-2364