施氮对水稻茎秆抗倒伏能力的影响及其形态和力学机理

【目的】研究施氮对水稻茎秆抗倒伏能力的影响及其与茎秆形态性状和力学性状的关系及其作用机理,为水稻抗倒高产栽培调控提供依据。【方法】以常规籼稻银晶软占为材料,设置4个氮水平,进行大田试验,研究施氮对水稻茎秆基部节间形态和力学性状的影响及其与倒伏指数的关系。【结果】施氮影响水稻茎秆的形态和力学性状。随着施氮量的增加,株高增加,重心上移,基部节间长度增加,节间充实度下降,抗折力和弹性模量减小,茎秆倒伏指数增加,抗倒伏能力下降。倒伏指数与株高、重心高度及基部节间长度呈正相关,而与基部节间充实度、抗折力及弹性模量呈负相关,且相关系数大多达到显著或极显著水平。倒伏指数与单位面积有效穗数和每穗总粒数呈显著或极显著正相关,而与结实率和千粒重呈负相关趋势。【结论】株高、重心高度、基部节间长度和基部节间充实度等形态性状,以及弯曲力矩、抗折力等力学性状,是影响水稻茎秆抗倒伏能力的主要因素。     

BNS和BNS366小麦雄性不育与内源激素的关系

【目的】研究BNS和BNS366小麦雄性不育与小孢子发生过程中内源激素含量变化的关系,为激素调控温光敏雄性不育小麦花粉育性提供理论依据。【方法】选用BNS和BNS366为试验材料,分别以矮抗58和郑麦366(BNS366的近等基因系)为对照,进行正常秋季播种(2018年10月10日)和晚播(2018年12月2日)试验,用I2-KI法测定花粉育性,用国内法和国际法测定自交结实率,采用间接酶联免疫法测定雌雄蕊原基分化期-三核期叶片、雌雄蕊原基分化期-四分体期幼穗和单核中位期-三核期花药中6种内源激素的含量。【结果】在正常秋季播种条件下,BNS和BNS366花粉可育率、国内法自交结实率和国际法自交结实率均为零,达到全不育水平,在晚播条件下,BNS的这三个指标分别为34.74%、43.12%和48.48%,达到低不育水平,BNS366则分别为92.63%、55.37%和67.94%,达到正常可育水平,播种期间差异均达到极显著水平;矮抗58和郑麦366在2种播期条件下,则为82.56%—94.00%、73.90%—82.31%和96.54%—139.26%,均为正常可育,播种期间差异不显著;BN...     

辣椒新品种‘苏椒 17 号’

 ‘苏椒17 号’是以母本‘06X354’与父本‘06X28’配制的早熟辣椒一代杂种。果实长灯笼 形,青果绿色,成熟果红色,果长10 ~ 11 cm,平均单果质量44.2 g,产量47 263.9 kg · hm-2,质脆微辣, 耐贮运,耐低温,高抗炭疽病,中抗病毒病,适合在江淮、黄淮流域保护地栽培。     

不同花生品种侧枝发育动态与产量性状研究

花生一级侧枝发育具有不均衡性。第一、二对侧枝出现早，伸长快，其他一级侧枝出现晚，伸长慢。86036－26－1第一、二对侧枝发育相对较早，而其他一级侧枝发育较晚；海花1号和豫花1号第一、二对发育相对较晚，而其他一级发育较快；豫花7号和鲁花9号居中。海花1号一级侧枝及第一对侧枝的二级侧枝数最多，86036－26－1的一级侧枝数及第一对侧枝的二级侧枝数量少。在花针期之前第一对侧枝的伸长与主茎基本同步；花针期之后，第一对侧枝伸长速度超过主茎。86036－26－1的一级侧枝长及株高居首位。86036－26－1第一、二对侧枝饱果率，产量居第一，豫花7号居第二。海花1号虽然荚果数较多，但第一、二对侧枝孢果率较低，产量也较低；鲁花9号居中。     

Comparison Analysis of Immune Related Gene of Orf Virus in Sheep and Camels

The purpose of this study was to investigate the variation of Orf virus (ORFV) immune related genes after infection with different species.The ORFV genomes of sheep and camel were extracted and named ORFV-Y and ORFV-LT,respectively.Based on ORFV genome sequence published in GenBank (accession No.:KF234407.1),three pairs of specific primers were designed and synthesized to amplify the B2L,F1L and VIR gene fragments of ORFV-Y and ORFV-LT,respectively,and the amplified fragments were cloned into pMD19-T vector,transformed into E.coli DH5α competent cells.The recombinant plasmid was identified,and positive clones were selected for sequencing,DNAStar software was used to analyze the homology,amino acid sequence and phylogenetic tree of 13 ORFV genome sequences published on NCBI.The results showed that the nucleotide homology of B2L,F1L and VIR genes were 92.8% to 99.2%,95.7% to 99.5% and 77.6% to 100%,respectively.After comparing the amino acid sequence between the two genomes and the reference sequence,it was found that there were obvious differences in the immune related genes between the two genomes,and F1L gene had some rules to follow.The phylogenetic analysis of B2L,F1L and VIR genes showed that ORFV-Y was closely related to the Chinese Fujian goat strain,while ORFV-LT was far from the reference strains,and was a separate branch.The results showed that ORFV had obvious difference in immune related genes between sheep and camels,it provided a reference basis for further research on the changes of ORFV gene sequences in different species and the development of vaccines for different species in the future.     

EGCG抗癌分子相关机理研究进展

表没食子儿茶素表没食子酸酯(EGCG)具有很好的抗癌活性,但其抗癌的分子机理较复杂,可通过多条途径抑制癌细胞生长或诱导癌细胞凋亡,其中较为关键的分子机理与EGCG的抗血管形成、阻滞癌细胞生长周期、抑制部分转录因子的表达以及调节细胞相关信号转导机制等有着密切的联系。综述了近年来国内外EDCG抗癌的分子机制研究进展。     

机动车废气分析仪信号处理系统的智能化设计

提出了机动车废气分析仪的智能化组成及其实现信号处理的全过程。详细阐述了信号处理的软硬件的实现。实验结果表明：本系统克服了传统不分光红外线分析仪固有的缺陷。进一步保证了测量数据的正确性和准确性。     

松毛虫赤眼蜂结合自走式高秆作物喷雾机防治玉米螟的效果

为探索防治二代玉米螟幼虫的新技术,在田间释放松毛虫赤眼蜂后,应用自走式高秆作物喷雾机喷药防治玉米螟.结果表明,此综合技术防治玉米螟的平均防治效果达64.22％,挽回玉米产量损失率11.20％,而仅用赤眼蜂防治一代玉米螟的平均防治效果为56.95％,挽回玉米产量损失率9.25％.因此,自走式高秆作物喷雾机喷药对玉米螟二代幼虫有控制效果.松毛虫赤眼蜂结合自走式高秆作物喷雾机喷药的综合防治技术能够减少田间玉米螟一代和二代幼虫数量,在玉米螟多代发生区可根据玉米螟发生情况和粮食生产的需要进行选用.     

The effects of 17β-estradiol on cardiomyocyte hypertrophy induced by endothelin

AIM:To investigate the effect of 17β-estradiol (E₂) on myocardial hypertrophy induced by endothelin-1(ET-1) and the related mechanism. METHODS:Myocardial cells from neonate rats were cultured in vitro and myocardial hypertrophy model was established with ET-1.The effects of 17β-estradiol on myocardial hypertrophy were observed. The role of ERK1/2 in the effects of 17β-estradiol was also detected. RESULTS:Compared with control group,ET-1 increased cell protein content,cell surface area and ［³H］^-Leucine ［³H］^-Leu) incorporation. Pretreatment with E₂ for 24 h could inhibit the increase in cell protein content,cell surface area and ［³H］^-Leu incorporation induced by ET-1.ET-1 significantly stimulated ERK1/2 activity,which was prevented by pretreatment with E₂.Tamoxifen,estradiol receptor antagonist,partially inhibited the effect of E₂. The ability of ET-1 to stimulate ［³H］^-Leu incorporation was significantly blocked by PD98059,which could enhance the inhibitory effect of E₂ on the increase of ［³H］^-Leu incorporation in cardiomyocytes induced by ET-1.CONCLUSION:E₂ can inhibit cardiomyocyte hypertrophy induced by ET-1. This effect is mediated by estrogen receptor. ERK1/2 signal pathway is closely correlated with the inhibitory effect of E₂ on cardiomyocyte hypertrophy induced by ET-1.     

出材量(率)调查方法的试验研究

通过2003年在小陇山林业实验局沙坝种子园人工商品林抚育采伐试点中的具体试验研究，较好地解决了在人工商品林抚育采伐中不伐树的情况下制定精度较高的出材量(率)量表用于估算出材量（率）这一难题，可供今后我省制定类似林业数表参考。